ER-076349
Based on 1 Customer Validation
ER-076349 (Eribulin intermediate) is an inhibitor of tubulin polymerization, induces G2-M cell cycle arrest, and disrupts mitotic spindles. ER-076349 inhibits cancer cell growth, and inhibits tumor growth in several human tumor xenografts. ER-076349 is an analog of Halichondrin B.
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- Reinheit: 98.44%
- CAS. Nr.: 253128-15-3
- Formel: C40H58O12
- Molecular Weight:730.88
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Speicherung:
-80°C, protect from light, stored under nitrogen
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| DLD-1 | IC50 |
0.54 nM
Compound: 1
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Antiproliferative activity against human DLD1 cells assessed as growth inhibition
Antiproliferative activity against human DLD1 cells assessed as growth inhibition
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[PMID: 21324686] |
| HCT-15 | IC50 |
0.22 nM
Compound: 1
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Antiproliferative activity against human HCT15 cells assessed as growth inhibition
Antiproliferative activity against human HCT15 cells assessed as growth inhibition
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[PMID: 21324686] |
| MES-SA | IC50 |
0.03 nM
Compound: 1
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Antiproliferative activity against human MES-SA cells assessed as growth inhibition
Antiproliferative activity against human MES-SA cells assessed as growth inhibition
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[PMID: 21324686] |
ER-076349 inhibits human cancer cell growth with IC50s of 0.59 nM (MDA-MB-435), 2.4 nM (COLO 205), 7.3 nM (DLD-1), 3.6 nM (DU 145), 1.8 nM (LNCaP), 3.2 nM (LOX ), 2.6 nM (HL-60), 4.0 nM (U937) respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 253128-15-3
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Appearance Solid
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Molecular Weight 730.88
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Formel C40H58O12
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Color White to off-white
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SMILES
C=C1C[C@@](CC[C@@]2(C[C@@]3([H])O4)O[C@]([C@](O[C@](C5)([H])CC6)([H])[C@@]6([H])O7)([H])[C@@]4([H])[C@]7([H])[C@@]3([H])O2)([H])O[C@@]1([H])CC[C@](C[C@@H](C)C8=C)([H])O[C@]8([H])C[C@@](O[C@H](C[C@H](O)CO)[C@@H]9OC)([H])[C@]9([H])CC5=O
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Synonyms
Eribulin intermediate
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Versand
Shipping with dry ice.
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Speicherung
-80°C, protect from light, stored under nitrogen
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (136.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.42 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.42 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (279 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3682 mL | 6.8411 mL | 13.6821 mL | 34.2053 mL |
| 5 mM | 0.2736 mL | 1.3682 mL | 2.7364 mL | 6.8411 mL | |
| 10 mM | 0.1368 mL | 0.6841 mL | 1.3682 mL | 3.4205 mL | |
| 15 mM | 0.0912 mL | 0.4561 mL | 0.9121 mL | 2.2804 mL | |
| 20 mM | 0.0684 mL | 0.3421 mL | 0.6841 mL | 1.7103 mL | |
| 25 mM | 0.0547 mL | 0.2736 mL | 0.5473 mL | 1.3682 mL | |
| 30 mM | 0.0456 mL | 0.2280 mL | 0.4561 mL | 1.1402 mL | |
| 40 mM | 0.0342 mL | 0.1710 mL | 0.3421 mL | 0.8551 mL | |
| 50 mM | 0.0274 mL | 0.1368 mL | 0.2736 mL | 0.6841 mL | |
| 60 mM | 0.0228 mL | 0.1140 mL | 0.2280 mL | 0.5701 mL | |
| 80 mM | 0.0171 mL | 0.0855 mL | 0.1710 mL | 0.4276 mL | |
| 100 mM | 0.0137 mL | 0.0684 mL | 0.1368 mL | 0.3421 mL |
ER-076349 Related Classifications
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