Art. -Nr.: HY-103466

FM4-64

22 Cited Publications

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Based on 22 publication(s) in Google Scholar

FM4-64 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM4-64 is widely used in endocytic and exospic membrane structure markers.

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Reinheit: 99.94%
CAS. Nr.: 162112-35-8
Formel: C₃₀H₄₅Br₂N₃
Molecular Weight:607.51

Speicherung:

-20°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

FM4-64 Chemical Structure

CAS. Nr.: 162112-35-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Publications (22)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Bioz

Biologische Aktivität

Beschreibung

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of FM working solution
1.1 Preparation of the stock solution
Dissolve FM in DMSO to obtain 5 mM of FM.
1.2 Preparation of FM working solution
Dilute the stock solution in HBSS to obtain 5-20 μM of FM working solution.
Note: Please adjust the concentration of FM working solution according to the actual situation.
2. Cell staining (Suspension cells)
2.1. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×10⁶/mL
2.2. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
2.3. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4. Wash twice with PBS, 5 minutes each time.
2.5. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
3. Cell staining (Adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2. Remove the coverslip from the medium and aspirate excess medium.
3.3. Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
3.4. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

730

Excitation (Ex)

510

Chemical Information

CAS. Nr. 162112-35-8
Appearance Solid
Molecular Weight 607.51
Formel C₃₀H₄₅Br₂N₃
Color Dark gray to black
SMILES

CC[N+](CCC[N+]1=CC=C(/C=C/C=C/C=C/C2=CC=C(N(CC)CC)C=C2)C=C1)(CC)CC.[Br-].[Br-]
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (22)

All Cell Imaging/Staining

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Journal Impact Factor

Nat Genet

The ZmWAKL-ZmWIK-ZmBLK1-ZmRBOH4 module provides quantitative resistance to gray leaf spot in maize. [Abstract]

2024 Feb;56(2):315-326. PMID: 38238629
Natl Sci Rev

A new type of cell related to organ movement for selfing in plants. [Abstract]

2023 Aug 10;10(9):nwad208. PMID: 37601240
Mol Cell

DUF4297 and HerA form abortosome to mediate bacterial immunity against phage infection. [Abstract]

2025 Mar 20;85(6):1176-1188.e5. PMID: 40010342
ACS Nano

Cryo-Shocked Platelet Coupled with ROS-Responsive Nanomedicine for Targeted Treatment of Thromboembolic Disease. [Abstract]

2023 Apr 11;17(7):6519-6533. PMID: 36971410
Nat Commun

Helical ultrastructure of the L-ENA spore aggregation factor of a Bacillus paranthracis foodborne outbreak strain. [Abstract]

2024 Aug 29;15(1):7514. PMID: 39209852
J Extracell Vesicles

Unveiling a Novel Mechanism of Enhanced Secretion, Cargo Loading, and Accelerated Dynamics of Bacterial Extracellular Vesicles Following Antibiotic Exposure. [Abstract]

2025 Jul;14(7):e70131. PMID: 40704571

FM4-64 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Jul;14(7):e70131. [Abstract]

FM4-64 (1 µM; 30 min). Confocal microscopy images of MG1655 stained with SYTOX (nucleic acid dye, green) and FM4‐64 (outer membrane dye, red) revealed that the SYTOX/FM4‐64 ratio increased under ENR treatment, indicating elevated membrane permeability.

FM4-64 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Jul;14(7):e70131. [Abstract]

FM4-64 (1 µM; 30 min). Fluorescence microscopy indicated no significant change in peptidoglycan integrity in ENR-treated ΔrecA strains compared to untreated controls.

FM4-64 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Jul;14(7):e70131. [Abstract]

FM4-64 (1 µM; 30 min). Fluorescence microscopy analysis using the SYTOX and FM4‐64 revealed increased cytoplasmic staining in both MG1655 and endolysin‐deficient strains (Δessd-1ΔrrrdΔrzod) under ENR stress, indicating membrane damage.
Autophagy

MoSec13 combined with MoGcn5b modulates MoAtg8 acetylation and regulates autophagy in Magnaporthe oryzae. [Abstract]

2025 May 9:1-18. PMID: 40320672
Adv Sci (Weinh)

A Trypsin-Like Serine Protease ZmNAL1a Fine-Tunes Maize Floral Transition and Flowering Time. [Abstract]

2026 Feb;13(10):e14635. PMID: 41486753
Adv Sci (Weinh)

Otilonium Bromide Exhibits Potent Antifungal Effects by Blocking Ergosterol Plasma Membrane Localization and Triggering Cytotoxic Autophagy in Candida Albicans. [Abstract]

2024 Jul 12:e2406473. PMID: 38995235

FM4-64 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 12:e2406473. [Abstract]

FM4-64 (1 µg/mL; 30 min). Confocal micrographs of vacuole staining (FM4‐64) and localization of Atg8 proteins in the GFP‐Atg8 mutant cells following the indicated treatment. DIC is Differential Interference Contrast; FM4‐64 was visualized as red fluorescent; Atg 8 proteins were visualized as green fluorescent.
Theranostics

Mitochondria-encoded peptide MOTS-c participates in plasma membrane repair by facilitating the translocation of TRIM72 to membrane. [Abstract]

2024 Aug 19;14(13):5001-5021. PMID: 39267782
Microbiome

Screening and identification of antimicrobial peptides from the gut microbiome of cockroach Blattella germanica. [Abstract]

2024 Dec 21;12(1):272. PMID: 39709489

FM4-64 purchased from MedChemExpress. Usage Cited in: Microbiome. 2024 Dec 21;12(1):272. [Abstract]

FM4-64 (20 μg/mL; 1 min). Representative images obtained from confocal laser scanning microscopy to analyze the behavior of AMP1. B. subtilis cells were treated with coumarin 343-labeled AMP1 at a concentration of 100 μM for various durations before observation. During observation, the B. subtilis cells membrane was stained with FM4-64.
Environ Sci Technol

Multiple Roles of Extracellular Vesicles in Promoting Microbial-Driven Manganese Reduction. [Abstract]

2025 Nov 4;59(43):23275-23288. PMID: 41115830
Plant Biotechnol J

Plasmodesmata-associated Flotillin positively regulates broad-spectrum virus cell-to-cell trafficking. [Abstract]

2024 May;22(5):1387-1401. PMID: 38130080
Plant Physiol

Long small RNA76113 targets CYCLIC NUCLEOTIDE-GATED ION CHANNEL 5 to repress disease resistance in rice. [Abstract]

2024 Feb 29;194(3):1889-1905. PMID: 37949839
Plant Physiol Biochem

The chitinase gene ZmChi7 confers salt stress tolerance by modulating the ROS homeostasis in maize. [Abstract]

2025 Dec 5:230:110906. PMID: 41365124
Plant J

Cucumber (Cucumis sativus L.) translationally controlled tumor protein interacts with CsRab11A and promotes activation of target of rapamycin in response to Podosphaera xanthii. [Abstract]

2024 Jul;119(1):332-347. PMID: 38700955
Virulence

Antibacterial efficacy and mechanism of the novel antimicrobial peptide lachnospirin-1 against Acinetobacter baumannii. [Abstract]

2026 Dec 31;17(1):2646808. PMID: 41838520
Virulence

The Hog1-Nmd5 signaling pathway regulates asexual development, lipid metabolism, stress response, trap morphogenesis, and secondary metabolism of Arthrobotrys oligospora. [Abstract]

2025 Dec;16(1):2468294. PMID: 39973133
Commun Biol

The structural and functional analysis of mycobacteria cysteine desulfurase-loaded encapsulin. [Abstract]

2024 Dec 19;7(1):1656. PMID: 39702509
Vet Microbiol

Sub-inhibitory gentamicin promotes extracellular vesicles biogenesis and blaNDM dissemination in carbapenem-resistant Escherichia coli via mrdA/mrdB pathway. [Abstract]

2025 Sep 2:310:110704. PMID: 40907215
Res Sq

2025 Sep 16.
biorxiv

2024 May 30.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (82.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6461 mL

8.2303 mL

16.4606 mL

5 mM

0.3292 mL

1.6461 mL

3.2921 mL

10 mM

0.1646 mL

0.8230 mL

1.6461 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. J E Gale, et al. FM1-43 dye behaves as a permeant blocker of the hair-cell mechanotransducer channel. J Neurosci. 2001 Sep 15;21(18):7013-25. [Content Brief]

[2]. Zal T, et al. Spectral shift of fluorescent dye FM4-64 reveals distinct microenvironment of nuclear envelope in living cells. Traffic. 2006;7(12):1607-1613. [Content Brief]

[3]. Stenovec M, et al. Distinct labelling of fusion events in rat lactotrophs by FM 1-43 and FM 4-64 is associated with conformational differences. Acta Physiol (Oxf). 2007;191(1):35-42. [Content Brief]

[4]. Bolte S, et al, Satiat-Jeunemaitre B. FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells. J Microsc. 2004;214(Pt 2):159-173. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6461 mL	8.2303 mL	16.4606 mL	41.1516 mL
	5 mM	0.3292 mL	1.6461 mL	3.2921 mL	8.2303 mL
	10 mM	0.1646 mL	0.8230 mL	1.6461 mL	4.1152 mL
	15 mM	0.1097 mL	0.5487 mL	1.0974 mL	2.7434 mL
	20 mM	0.0823 mL	0.4115 mL	0.8230 mL	2.0576 mL
	25 mM	0.0658 mL	0.3292 mL	0.6584 mL	1.6461 mL
	30 mM	0.0549 mL	0.2743 mL	0.5487 mL	1.3717 mL
	40 mM	0.0412 mL	0.2058 mL	0.4115 mL	1.0288 mL
	50 mM	0.0329 mL	0.1646 mL	0.3292 mL	0.8230 mL
	60 mM	0.0274 mL	0.1372 mL	0.2743 mL	0.6859 mL
	80 mM	0.0206 mL	0.1029 mL	0.2058 mL	0.5144 mL

Keywords

FM4-64

22 Cited Publications

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FM4-64

Publications Citing Use of MedChemExpress (MCE) FM4-64

Biologische Aktivität

Chemical Information

Publications (22)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

FM4-64 Related Classifications

Art. -Nr.: HY-103466