Art. -Nr.: HY-119696

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MTIC

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MTIC, the active metabolite of Temozolomide (TMZ), is a DNA alkylating agent. MTIC has antitumor activity .

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Reinheit: 98.83%
CAS. Nr.: 3413-72-7
Formel: C₅H₈N₆O
Molecular Weight:168.16

Speicherung:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

MTIC Chemical Structure

CAS. Nr.: 3413-72-7

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

MTIC, the active metabolite of Temozolomide (TMZ), is a DNA alkylating agent. MTIC has antitumor activity ^[1]^[2]^[3]^[4].

Cellular Effect

Cell Line	Type	Value	Description	References
GM892A cell line	IC₅₀	5 μM Compound: 4	In vitro cytotoxicity against human lymphoblastoid cell (GM892 A) In vitro cytotoxicity against human lymphoblastoid cell (GM892 A)	[PMID: 7739008]
Raji	IC₅₀	101 μM Compound: 4	In vitro cytotoxicity against Raji cell line In vitro cytotoxicity against Raji cell line	[PMID: 7739008]
SNB-19	GI₅₀	466 μM Compound: 3; MTIC	Growth inhibition of MGMT-transfected human SNB19 cells after 7 days by MTT assay Growth inhibition of MGMT-transfected human SNB19 cells after 7 days by MTT assay	10.1039/C6MD00384B
SNB-19	GI₅₀	472 μM Compound: 2; MTIC	Growth inhibition of MGMT-transfected human SNB19 cells expressing MGMT after 7 days by MTT assay Growth inhibition of MGMT-transfected human SNB19 cells expressing MGMT after 7 days by MTT assay	[PMID: 30108945]
SNB-19	GI₅₀	48.5 μM Compound: 2; MTIC	Growth inhibition of vehicle-transfected human SNB19 cells after 7 days by MTT assay Growth inhibition of vehicle-transfected human SNB19 cells after 7 days by MTT assay	[PMID: 30108945]
SNB-19	GI₅₀	48.5 μM Compound: 3; MTIC	Growth inhibition of empty vector transfected human SNB19 cells after 7 days by MTT assay Growth inhibition of empty vector transfected human SNB19 cells after 7 days by MTT assay	10.1039/C6MD00384B
U-373MG ATCC	GI₅₀	398 μM Compound: 2; MTIC	Growth inhibition of MGMT-transfected human U373 cells expressing MGMT after 7 days by MTT assay Growth inhibition of MGMT-transfected human U373 cells expressing MGMT after 7 days by MTT assay	[PMID: 30108945]
U-373MG ATCC	GI₅₀	398 μM Compound: 3; MTIC	Growth inhibition of MGMT-transfected human U373 cells after 7 days by MTT assay Growth inhibition of MGMT-transfected human U373 cells after 7 days by MTT assay	10.1039/C6MD00384B
U-373MG ATCC	GI₅₀	93.7 μM Compound: 2; MTIC	Growth inhibition of vehicle-transfected human U373 cells after 7 days by MTT assay Growth inhibition of vehicle-transfected human U373 cells after 7 days by MTT assay	[PMID: 30108945]
U-373MG ATCC	GI₅₀	93.7 μM Compound: 3; MTIC	Growth inhibition of empty vector transfected human U373 cells after 7 days by MTT assay Growth inhibition of empty vector transfected human U373 cells after 7 days by MTT assay	10.1039/C6MD00384B

In Vivo

MTIC (cumulative dose=890 mg/rat/14 wk) can induce a high incidence of breast adenofibroma and a low incidence of uterine leiomyosarcoma^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 3413-72-7
Appearance Solid
Molecular Weight 168.16
Formel C₅H₈N₆O
Color Off-white to pink
SMILES

O=C(C1=C(/N=N/NC)N=CN1)N
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 25 mg/mL (148.67 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.9467 mL

29.7336 mL

59.4672 mL

5 mM

1.1893 mL

5.9467 mL

11.8934 mL

10 mM

0.5947 mL

2.9734 mL

5.9467 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (14.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (14.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (14.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Ramalho MJ, et al. Biophysical interaction of temozolomide and its active metabolite with biomembrane models: The relevance of drug-membrane interaction for Glioblastoma Multiforme therapy. Eur J Pharm Biopharm. 2019;136:156-163. [Content Brief]

[2]. Nagasawa HT, et al. The mechanism of alkylation of DNA by 5-(3-methyl-1-triazeno)imidazole-4-carboxamide (MIC), a metabolite of DIC (NSC-45388). Non-involvement of diazomethane. Chem Biol Interact. 1974 Jun;8(6):403-13. [Content Brief]

[3]. Tsang LL, et al. Comparison of the cytotoxicity in vitro of temozolomide and dacarbazine, prodrugs of 3-methyl-(triazen-1-yl)imidazole-4-carboxamide. Cancer Chemother Pharmacol. 1991;27(5):342-6. [Content Brief]

[4]. Beal DD, et al. Carcinogenicity of the antineoplastic agent, 5-(3,3-dimethyl-1-triazeno)-imidazole-4-carboxamide, and its metabolites in rats. J Natl Cancer Inst. 1975 Apr;54(4):951-7. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.9467 mL	29.7336 mL	59.4672 mL	148.6679 mL
	5 mM	1.1893 mL	5.9467 mL	11.8934 mL	29.7336 mL
	10 mM	0.5947 mL	2.9734 mL	5.9467 mL	14.8668 mL
	15 mM	0.3964 mL	1.9822 mL	3.9645 mL	9.9112 mL
	20 mM	0.2973 mL	1.4867 mL	2.9734 mL	7.4334 mL
	25 mM	0.2379 mL	1.1893 mL	2.3787 mL	5.9467 mL
	30 mM	0.1982 mL	0.9911 mL	1.9822 mL	4.9556 mL
	40 mM	0.1487 mL	0.7433 mL	1.4867 mL	3.7167 mL
	50 mM	0.1189 mL	0.5947 mL	1.1893 mL	2.9734 mL
	60 mM	0.0991 mL	0.4956 mL	0.9911 mL	2.4778 mL
	80 mM	0.0743 mL	0.3717 mL	0.7433 mL	1.8583 mL
	100 mM	0.0595 mL	0.2973 mL	0.5947 mL	1.4867 mL

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MTIC

Temozolomide

Staurosporine

Phosphoramide mustard cyclohexanamine

IBMX

Olaparib

Ruxolitinib

Dihydrorhodamine 123

Prednisolone

Doxorubicin hydrochloride

Dexamethasone

Y-27632 dihydrochloride

Palbociclib

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

MTIC

Temozolomide

Staurosporine

Phosphoramide mustard cyclohexanamine

IBMX

Olaparib

Ruxolitinib

Dihydrorhodamine 123

Prednisolone

Doxorubicin hydrochloride

Dexamethasone

Y-27632 dihydrochloride

Palbociclib

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

MTIC Related Classifications

HY-119696 Related Classifications

Bioz