Art. -Nr.: HY-131042

NNMTi

12 Cited Publications

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Based on 12 publication(s) in Google Scholar

NNMTi is a potent nicotinamide N-methyltransferase (NNMT) inhibitor (IC₅₀=1.2 μM) and selectively binds to the NNMT substrate-binding site residues. NNMTi promotes myoblast differentiation in vitro and enhances fusion and regenerative capacity of muscle stem cells (muSCs) in aged mice.

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Reinheit: 99.70%
CAS. Nr.: 42464-96-0
Formel: C₁₀H₁₁IN₂
Molecular Weight:286.11

Speicherung:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

NNMTi Chemical Structure

CAS. Nr.: 42464-96-0

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Publications (12)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

NNMTi is a potent nicotinamide N-methyltransferase (NNMT) inhibitor (IC₅₀=1.2 μM) and selectively binds to the NNMT substrate-binding site residues^[2]. NNMTi promotes myoblast differentiation in vitro and enhances fusion and regenerative capacity of muscle stem cells (muSCs) in aged mice^[1].

Alle Amine N-methyltransferase Isoform-spezifische Produkte anzeigen :

PNMT
NNMT

In Vitro

NNMTi (10-30 μM; 96 hours) produces a concentration-related increase in myoblast differentiation on C2C12 myoblast differentiation. 30 μM NNMTi results in 18% MHC-positive myotube nuclei, representing a 45% increase in the extent of myoblast differentiation compared to untreated differentiating myoblasts (12% MHC-positive myotube nuclei)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

NNMTi (subcutaneous (SC) injection; 5 mg/kg and 10 mg/kg; 2 weeks (1 week pre-injury and 1 week post-injury)) has an effect on muscle regeneration after injury, it results in 60% and 75% higher incidence of proliferating/active muSCs at 5 mg/kg and 10 mg/kg, respectively. The relative numbers of fibers with an EdU⁺ myonucleus increased 40% and 48% with NNMTi treatment at 5 mg/kg and 10 mg/kg, respectively. The odds ratio of fused myonuclei for control are 0.58 and 0.53 times the odds at the low and high NNMTi dose, respectively^[2].
NNMTi (subcutaneous injection; 10 mg/kg; 1 week) produces no systemic toxicity in mice, the levels of the glucose, cholesterol, plasma proteins, and electrolytes between control and NNMTi-treated samples show no difference in mice. 1-week post-injury daily repeat-dosing of NNMTi is well tolerated with no untoward systemic toxicity or behavioral implications in aged mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 42464-96-0
Appearance Solid
Molecular Weight 286.11
Formel C₁₀H₁₁IN₂
Color Brown to reddish brown
SMILES

C[N+]1=C2C=CC=C(N)C2=CC=C1.[I-]
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture

* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Publications (12)

All In Vivo Efficacy StudyIHCWBRT-PCR

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Journal Impact Factor

Mol Cancer

NNMT promotes acquired EGFR-TKI resistance by forming EGR1 and lactate-mediated double positive feedback loops in non-small cell lung cancer. [Abstract]

2025 Mar 15;24(1):79. PMID: 40089784

NNMTi purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 Mar 15;24(1):79. [Abstract]

NNMTi (10 mg/kg; i.p.; once daily) treatment effectivelyreversed EGR1 or ALDH3A1 overexpression-promoted tumor growth of BALB/c nude mice.

NNMTi purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 Mar 15;24(1):79. [Abstract]

NNMTi (10 mg/kg; i.p.; once daily) reversed the expression of Ki67 induced by the overexpression of EGR1 or ALDH3A1 in BALB/c nude mice.

NNMTi purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 Mar 15;24(1):79. [Abstract]

WB assays were performed following administration of NNMTi (10-40 mg/kg) to osimertinib-resistant cells (PC9/OR and HCC827/OR).
Cell Metab

Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer. [Abstract]

2024 Jun 18:S1550-4131(24)00189-X. PMID: 38897198

NNMTi purchased from MedChemExpress. Usage Cited in: Cell Metab. 2024 Jun 18:S1550-4131(24)00189-X. [Abstract]

NNMTi (10 μM; 48 h) reversed the upregulation of EMT markers (Vimentin and N-cadherin) in MFC induced by coculture with NNMT-overexpressing CAFs.

NNMTi purchased from MedChemExpress. Usage Cited in: Cell Metab. 2024 Jun 18:S1550-4131(24)00189-X. [Abstract]

Expression of tumor-promoting markers (Vimentin, Vegfa, II10, and Tgfb1) in mouse subcutaneous tumor-isolated CAFs (m-CAFs) or Nnmt-overexpressing (oxNnmt) m-CAFs treated with NNMT inhibitor NNMTi (10 mM, 48 h) was detected by RT-qPCR.
Gut

NNMT enriches for AQP5+ cancer stem cells to drive malignant progression in early gastric cardia adenocarcinoma. [Abstract]

2023 Dec 7;73(1):63-77. PMID: 36977555
Genes Dis

2026 May 27.
Cell Commun Signal

NAMPT and NNMT released via extracellular vesicles and as soluble mediators are distinguished traits of BRAF inhibitor resistance of melanoma cells impacting on the tumor microenvironment. [Abstract]

2025 Jul 21;23(1):348. PMID: 40691620
Oncogene

Enhancing glioma immunotherapy by disrupting RBP-J-mediated NNMT signaling in tumor microenvironment. [Abstract]

2026 Aug;45(29):2959-2977. PMID: 42310095
Aging Cell

Identification of nicotinamide N-methyltransferase as a promising therapeutic target for sarcopenia. [Abstract]

2024 Jun 5:e14236. PMID: 38838088
Cell Death Discov

Insufficient NNMT promotes autophagy and disrupts progesterone signaling in endometrial stromal cells in recurrent implantation failure by modulating the H3K9me3-ALDH1A3 pathway. [Abstract]

2025 Oct 7;11(1):450. PMID: 41057307
Int Immunopharmacol

Nicotinamide N-methyltransferase mediates redox regulation and colonic epithelial barrier impairment by SIRT1/PPAR-γ/NLRP6/NRF2 axis in inflammatory bowel disease. [Abstract]

2026 Jan 1;168(Pt 2):115882. PMID: 41275826
Toxicology

NNMT is involved in deoxynivalenol-induced hepatocyte toxicity via promoting ferroptosis. [Abstract]

2025 May:513:154084. PMID: 39983889
J Diabetes Complications

Nicotinamide n-methyltransferase inhibitor synergizes with sodium-glucose cotransporter 2 inhibitor to protect renal tubular epithelium in experimental models of type 2 diabetes mellitus. [Abstract]

2025 Jan 18;39(2):108952. PMID: 39848127
Physiol Rep

Nicotinamide N-methyltransferase inhibition improves limb function in experimental peripheral artery disease. [Abstract]

2025 Oct;13(20):e70615. PMID: 41108586

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 20.83 mg/mL (72.80 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 2.27 mg/mL (7.93 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.4952 mL

17.4758 mL

34.9516 mL

5 mM

0.6990 mL

3.4952 mL

6.9903 mL

10 mM

0.3495 mL

1.7476 mL

3.4952 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (7.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (7.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Harshini Neelakantan, et al. Small molecule nicotinamide N-methyltransferase inhibitor activates senescent muscle stem cells and improves regenerative capacity of aged skeletal muscle. Biochem Pharmacol. 2019 May;163:481-492. [Content Brief]

[2]. Harshini Neelakantan, et al. Structure-Activity Relationship for Small Molecule Inhibitors of Nicotinamide N-Methyltransferase. J Med Chem [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	3.4952 mL	17.4758 mL	34.9516 mL	87.3790 mL
H₂O / DMSO	5 mM	0.6990 mL	3.4952 mL	6.9903 mL	17.4758 mL
DMSO	10 mM	0.3495 mL	1.7476 mL	3.4952 mL	8.7379 mL
	15 mM	0.2330 mL	1.1651 mL	2.3301 mL	5.8253 mL
	20 mM	0.1748 mL	0.8738 mL	1.7476 mL	4.3689 mL
	25 mM	0.1398 mL	0.6990 mL	1.3981 mL	3.4952 mL
	30 mM	0.1165 mL	0.5825 mL	1.1651 mL	2.9126 mL
	40 mM	0.0874 mL	0.4369 mL	0.8738 mL	2.1845 mL
	50 mM	0.0699 mL	0.3495 mL	0.6990 mL	1.7476 mL
	60 mM	0.0583 mL	0.2913 mL	0.5825 mL	1.4563 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

NNMTi

12 Cited Publications

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NNMTi

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Targeted Diversity Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) NNMTi

Alle Amine N-methyltransferase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (12)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

NNMTi

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Targeted Diversity Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) NNMTi

Alle Amine N-methyltransferase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (12)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

NNMTi Related Classifications

HY-131042 Related Classifications