Art. -Nr.: HY-125427

SKOG102

Name: SKOG102
Brand: MedChemExpress
SKU: HY-125427
Price: 525.00 USD
Availability: InStock
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SKOG102 is a potent OLIG2 inhibitor that is directly engaging OLIG2 and interferes with the ability of OLIG2 to bind DNA. SKOG102 can be used for the research of glioblastoma (GBM).

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.09%
CAS. Nr.: 21062-28-2
Formel: C₁₉H₂₅Cl₂N₇
Molecular Weight:422.35

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

SKOG102 Chemical Structure

CAS. Nr.: 21062-28-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

SKOG102 is a potent OLIG2 inhibitor that is directly engaging OLIG2 and interferes with the ability of OLIG2 to bind DNA. SKOG102 can be used for the research of glioblastoma (GBM)^[1].

IC₅₀ & Target

OLIG2^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
PC-12	IC₅₀	1.1 μM Compound: 18, TKO-10-20	Inhibition of nicotinic cholinergic receptor in rat PC12 cells assessed as inhibition of nicotine-stimulated [3H]-norepinephrine release after 30 mins by liquid scintillation counting analysis Inhibition of nicotinic cholinergic receptor in rat PC12 cells assessed as inhibition of nicotine-stimulated [3H]-norepinephrine release after 30 mins by liquid scintillation counting analysis	[PMID: 23916146]

In Vitro

SKOG102 (0.1-5 μM; 72 h) inhibits human GBM4 and GBM8 cells grown as neurospheres, with IC₅₀s of 10.66 and 1.536 μM, respectively^[1].
SKOG102 (0.5-5 μM; 12 h) increases p21 RNA levels and decreases OMG RNA levels in GBM4 cells^[1].
SKOG102 (5 μM; 20 h) suppresses OLIG2-DNA binding, and decreases the serine phosphorylated OLIG2 and total OLIG2 protein levels in GBM8 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SKOG102 (10-20 mg/kg; i.p.) inhibits GBM4 tumor growth in mice^[1].
SKOG102 (5 mg/kg; i.p.) accumulates in the tumor even over a relatively brief time period and the plasma and brain concentrations are almost identical at 4 hours after injection in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

NSG mice were inoculated in each flank with GBM4 cells^[1]
Dosage:

10, 15, and 20 mg/kg; dissolved in solutol:PEG400:water (20%:40%:40%)
Administration:

Intraperitoneal injection; first 2 weeks (from day 33 to 46) 10 mg/kg, third week (from day 47 to 54) 15 mg/kg, fourth week (from 55 to 66) first 3 days 20 mg/kg, 2 days break, then alternate days from day 60 to 66
Result:

Significantly attenuated tumor growth.

Chemical Information

CAS. Nr. 21062-28-2
Appearance Solid
Molecular Weight 422.35
Formel C₁₉H₂₅Cl₂N₇
Color White to off-white
SMILES

N=C(NC1=NC(C)=CC(NC2CN(CC)CCC2)=N1)NC3=CC=C(Cl)C(Cl)=C3
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (236.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3677 mL

11.8385 mL

23.6770 mL

5 mM

0.4735 mL

2.3677 mL

4.7354 mL

10 mM

0.2368 mL

1.1839 mL

2.3677 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 6.25 mg/mL (14.80 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Tsigelny IF, et, al. Multiple spatially related pharmacophores define small molecule inhibitors of OLIG2 in glioblastoma. Oncotarget. 2017 Apr 4;8(14):22370-22384. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3677 mL	11.8385 mL	23.6770 mL	59.1926 mL
	5 mM	0.4735 mL	2.3677 mL	4.7354 mL	11.8385 mL
	10 mM	0.2368 mL	1.1839 mL	2.3677 mL	5.9193 mL
	15 mM	0.1578 mL	0.7892 mL	1.5785 mL	3.9462 mL
	20 mM	0.1184 mL	0.5919 mL	1.1839 mL	2.9596 mL
	25 mM	0.0947 mL	0.4735 mL	0.9471 mL	2.3677 mL
	30 mM	0.0789 mL	0.3946 mL	0.7892 mL	1.9731 mL
	40 mM	0.0592 mL	0.2960 mL	0.5919 mL	1.4798 mL
	50 mM	0.0474 mL	0.2368 mL	0.4735 mL	1.1839 mL
	60 mM	0.0395 mL	0.1973 mL	0.3946 mL	0.9865 mL
	80 mM	0.0296 mL	0.1480 mL	0.2960 mL	0.7399 mL
	100 mM	0.0237 mL	0.1184 mL	0.2368 mL	0.5919 mL

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SKOG102