Avutometinib potassium

Avutometinib potassium purchased from MedChemExpress. Usage Cited in: Nat Cancer. 2026 Jan;7(1):116-130. [Abstract]

Customer Validation
Western blot analysis of MEK immunoprecipitants depicting the modulation of MEK–BRAF and MEK–CRAF interactions in HCT-116 cells treated with DMSO, trametinib (10 nM), Avutometinib (30 nM), trametiglue (3 nM) or IK-595 (3 nM) for 4 h. A representative image of three independent experiments is shown.

Avutometinib potassium purchased from MedChemExpress. Usage Cited in: Nat Cancer. 2026 Jan;7(1):116-130. [Abstract]

Customer Validation
DMSO–inhibitor K_D ratios obtained from an AlphaLISA biochemical assay measuring the interaction between MEK1 and BRAF (top) or CRAF (bottom) proteins following treatment with IK-595 (n = 1 sample per condition with nine biological replicates), trametinib (n = 1 sample per condition with five biological replicates) or Avutometinib (0.01-1 μM; 30 min) (n = 1 sample oer condition with four biological replicates). The results demonstrated that IK-595 and Avutometinib stabilized MEK1 with both BRAF and CRAF, whereas trametinib disrupted these interactions.

Avutometinib potassium purchased from MedChemExpress. Usage Cited in: Nat Cancer. 2026 Jan;7(1):116-130. [Abstract]

Customer Validation
Western blot quantification of MEK phosphorylation normalized to total MEK protein levels in HCT-116 cells treated with DMSO, Avutometinib (30 nM), trametinib (10 nM), trametiglue (3 nM), mirdametinib (25 nM), binimetinib (50 nM), selumetinib (550 nM), cobimetinib (320 nM) or IK-595 (3 nM) for 4 or 48 h (n = 1 sample per condition in two biological replicates).

Avutometinib potassium purchased from MedChemExpress. Usage Cited in: Sci Data. 2024 Sep 19;11(1):1024. [Abstract]

Customer Validation
CEP-32496, Avutometinib (Ro 5126766) (0.1-10 μM; 1 h) and PLX8394 didn’t inhibit necroptosis in L929 cells. L929 or HT-29 cells were pretreated with DMSO or Nec-1 or indicated inhibitors for 1h, then stimulated with T/Z for 3 hours or T/S/Z for 8 hours, respectively. Then cell viability was determined by CCK8 assay.