BI-2493

Name: BI-2493
Brand: MedChemExpress
SKU: HY-153723
Rating: 5.0 (4 reviews)

Cat. No.: HY-153723 Purity: 99.79%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

BI-2493 is a structural analogue of BI-2865 and a highly selective and orally active pan-KRAS inhibitor. BI-2493 can attenuate tumor growth. BI-2493 can be used for cancer iseases research.

For research use only. We do not sell to patients.

BI-2493 Chemical Structure

CAS No. : 2937344-16-4

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

4 Publications Citing Use of MCE BI-2493

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K-Ras H-Ras N-Ras Ras

Biological Activity
Purity & Documentation
References
Customer Review

Description

BI-2493 is a structural analogue of BI-2865 and a highly selective and orally active pan-KRAS inhibitor. BI-2493 can attenuate tumor growth. BI-2493 can be used for cancer iseases research^[1].

IC₅₀ & Target

K-Ras WT

In Vitro

BI-2493 (0-10 μM, 72 h) inhibits the proliferation of KRAS mutant cancer cell lines in a concentration-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	ASPC1 cells (G12D), HPAC cells (G12D), H727 cells (G12V) (BaF3 cells transduced with a virus derived from plasmids expressing KRAS G12D or G12V mutants)
Concentration:	0, 100 nM, 1000 nM, 10000 nM
Incubation Time:	72 h
Result:	Effectively inhibited the proliferation of cancer cells ASPC1 (G12D), HPAC (G12D), and H727 (G12V)

In Vivo

BI-2493 (90 mg/kg, p.o., twice daily for 21 days) exhibits tumor-suppressive activity in LS513/GP2d/HPAC/SW620/MIAPACA2-derived xenograft NMRI mice models^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	LS513/GP2d/HPAC/SW620/MIAPACA2-derived xenograft NMRI mice models
Dosage:	90 mg/kg
Administration:	Oral gavage (p.o.), twice daily for 21 days
Result:	Reduced tumor growth in mice carrying KRAS G12C, G12D, G12V, and A146V mutation models, without causing significant toxicity to the mice.

Molecular Weight

461.58

Formula

C₂₄H₂₇N₇OS

CAS No.

2937344-16-4

Appearance

Solid

Color

Off-white to yellow

SMILES

N#CC1=C(N)SC2=C1[C@]3(CCC2)C4=C(CCC3)C(C5=NC(N6[C@H](CNCC6)C)=NC=C5)=NO4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (108.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1665 mL	10.8324 mL	21.6647 mL
5 mM	0.4333 mL	2.1665 mL	4.3329 mL
10 mM	0.2166 mL	1.0832 mL	2.1665 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.71 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.71 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 1.25 mg/mL (2.71 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.79%

Select Batch:

Data Sheet (276 KB) SDS (254 KB)

COA (253 KB) HNMR (139 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Kim D, et al. Pan-KRAS inhibitor disables oncogenic signalling and tumour growth. Nature. 2023 Jul;619(7968):160-166. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1665 mL	10.8324 mL	21.6647 mL	54.1618 mL
	5 mM	0.4333 mL	2.1665 mL	4.3329 mL	10.8324 mL
	10 mM	0.2166 mL	1.0832 mL	2.1665 mL	5.4162 mL
	15 mM	0.1444 mL	0.7222 mL	1.4443 mL	3.6108 mL
	20 mM	0.1083 mL	0.5416 mL	1.0832 mL	2.7081 mL
	25 mM	0.0867 mL	0.4333 mL	0.8666 mL	2.1665 mL
	30 mM	0.0722 mL	0.3611 mL	0.7222 mL	1.8054 mL
	40 mM	0.0542 mL	0.2708 mL	0.5416 mL	1.3540 mL
	50 mM	0.0433 mL	0.2166 mL	0.4333 mL	1.0832 mL
	60 mM	0.0361 mL	0.1805 mL	0.3611 mL	0.9027 mL
	80 mM	0.0271 mL	0.1354 mL	0.2708 mL	0.6770 mL
	100 mM	0.0217 mL	0.1083 mL	0.2166 mL	0.5416 mL

BI-2493 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BI-24932937344-16-4BI2493BI 2493RasASPC1 cells (G12D)HPAC cells (G12D)H727 cells (G12V)Anti-cancer activityInhibitorinhibitorinhibit

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BI-2493

Customer Review

BI-2493 Related Antibodies

4 Publications Citing Use of MCE BI-2493

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Ras Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

BI-2493 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

BI-2493 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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