2. TGF-beta/Smad
  3. TGF-β Receptor
  4. Bimagrumab

Bimagrumab  (Synonyms: Anti-ACVR2B Reference Antibody; BYM338)

Cat. No.: HY-P99355 Purity: 99.31%
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Bimagrumab (Anti-ACVR2B Reference Antibody) is a human monoclonal antibody that blocks activin type II receptor (ActRII), with KDs of 1.7 pM and 434 pM for human ActRIIB and ActRIIA, respectively. Bimagrumab can be used for the research of pathological muscle loss and weakness.

For research use only. We do not sell to patients.

CAS No. : 1356922-05-8

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Customer Review

Based on 3 publication(s) in Google Scholar

Bimagrumab Featured Recommendations:

Bimagrumab Related Antibodies

Top Publications Citing Use of Products
IF
In Vivo Efficacy Study
WB
Histological Imaging/Staining

    Bimagrumab purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 1;16(1):5976.  [Abstract]

    Representative microscopy images showing C2C12 myoblast differentiation in the presence of Myostatin, soluble DLK1 + Myostatin, Bimagrumab + Myostatin, or Bimagrumab + soluble DLK1 + Myostatin. Control cells were allowed to differentiate for 72h. Myostatin treatment (4 µg/ml) inhibits C2C12 myoblast differentiation into myotubes as determined by MyoHC staining. Bimagrumab (100 nM) and soluble DLK1 (2 μM) reverse the effect of myostatin on C2C12 differentiation, with no additive effect of adding soluble DLK1 to Bimagrumab treated cells. C2C12 cells were fixed with 4% PFA, immunostained with an anti-MyoHC antibody and an anti-mouse Alexa Fluor 488 secondary antibody. Nuclei were counterstained with Hoechst 33342. Nuclei represented with pseudo color (magenta). The images are representative of one out of two imaging wells per treatment from one experiment. The experiment was independently repeated two times. Scale bar, 100 μm.

    Bimagrumab purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Sep 30;16(1):8654.  [Abstract]

    Pancreatic tumor samples from chow (n = 6 mice per group) and diabetic (n = 9 mice per group) KPC tumor-bearing mice treated with IgG, bimagrumab, or bimagrumab+metformin. The results showed that two injections of bimagrumab (20 mg/kg, subcutaneously, once weekly) reduced tumor burden in diabetic mice.

    Bimagrumab purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Sep 30;16(1):8654.  [Abstract]

    Phosphorylated Smad2 levels and total Smad2 expression were detected via western blotting in KPC tumors from the chow and diabetic mice treated with IgG or bimagrumab (20 mg/kg, subcutaneous injection, once weekly for two weeks) (n=3 mice per group).

    Bimagrumab purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Sep 30;16(1):8654.  [Abstract]

    Representative images of picrosirius red staining (red) in tumors from chow and diabetic KPC tumor-bearing mice treated with IgG or bimagrumab (20 mg/kg, subcutaneous injection, once weekly for two weeks) (n = 6 mice per group).

    Bimagrumab purchased from MedChemExpress. Usage Cited in: Mol Metab. 2024 Feb:80:101880.  [Abstract]

    Bimagrumab (20 mg/kg, subcutaneous injection, once weekly for two weeks). Representative TA sections stained with H&E (20x magnification, scale bar = 100 um).

    View All TGF-β Receptor Isoform Specific Products:

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    ALK1 ALK2 ALK3 ALK4 ALK5 ALK6 ALK7 TGFβR2 ACVR2A ACVR2B BMP

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    Description

    Bimagrumab (Anti-ACVR2B Reference Antibody) is a human monoclonal antibody that blocks activin type II receptor (ActRII), with KDs of 1.7 pM and 434 pM for human ActRIIB and ActRIIA, respectively. Bimagrumab can be used for the research of pathological muscle loss and weakness[1][2].

    Isotype

    Human IgG1 (L234A/L235A) lambda2
    Recommend Isotype Controls
    Species Reactivity

    Human
    IC50 & Target[2]

    ACVR2B

    1.7 pM (Kd)

    ACVR2A

    434 pM (Kd)

    In Vitro

    BYM338 (1 pM-1 μM; 24 h) inhibits Myostatin- and Activin A-induced Smad2/3-dependent luciferase responses in HEK293 cells with IC50s of 154 and 343 pM, respectively[2].
    BYM338 (4 days) prevents the inhibition of differentiation and fusion induced upon exogenous addition of Myostatin or Activin A[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Bimagrumab Related Antibodies
    In Vivo

    BYM338 (6-20 mg/kg; s.c. once weekly for 4 weeks) promotes an increase in body weight and skeletal muscle hypertrophy of all examined muscles, slow, fast, and mixed of young SCID mice[2].
    BYM338 (5-20 mg/kg; s.c. once weekly for 14 d) prevents Glucocorticoid-induced muscle wasting in mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Gene ID

    93  [NCBI]

    Accession
    Application

    ELISA, FACS, Functional assay
    Conjugated

    Unconjugated
    Reconsititution

    The product can be reconstituted/diluted with sterile PBS or saline.
    Molecular Weight

    142.78 kDa

    CAS No.
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    [Bimagrumab]
    Shipping

    Shipping with dry ice.
    Formulation

    Please refer to the lot-specific COA for specific buffer information.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Format
    • Human IgG1 lambda
    Biological Activity
    • Immobilized ACVR2B Protein, Human can bind Bimagrumab. The EC50 for this effect is 8.254 ng/mL.
    • Flow cytometric analysis of 106 Jurkat cells with Bimagrumab (HY-P99355, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 lambda1 (HY-P99992, blue) was used as the isotype control.
    Purity & Documentation

    Purity: 99.31%

    SDS (252 KB)

    COA (232 KB) SDS-PAGE (82 KB) SEC-HPLC (147 KB)

    Inhibitory Antibodies User Guide (603 KB)
    References
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    • If you have published this work, please enter the PubMed ID.
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    Bimagrumab Related Classifications

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Bimagrumab1356922-05-8Anti-ACVR2B Reference Antibody BYM338BYM338BYM 338BYM-338TGF-β ReceptorTransforming growth factor beta receptorsACVR2BACVR2AActRIIBActRIIAmuscle lossmuscle weaknessInhibitorinhibitorinhibit

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