Blasticidin A  (Synonyms: (+)-Blasticidin A)

Blasticidin A is a tetraamide acid derivative antibiotic produced by Streptomyces griseochromogenes, as well as a selective inhibitor of aflatoxin production. Blasticidin A exhibits antimicrobial activity against yeast. Blasticidin A can be used in research related to aflatoxin contamination (infection by Aspergillus sp.)^[1][2].

Blasticidin A (0.25-0.5 μM) dose-dependently inhibits aflatoxin production by Aspergillus parasiticus NRRL2999 in liquid culture, achieving near-complete inhibition at 0.5 μM without significantly reducing mycelial growth^[1].
Blasticidin A (0.03-0.5 μg/mL) dose-dependently inhibits aflatoxin production by Aspergillus parasiticus NRRL2999 on agar plates, achieving complete inhibition at 0.5 μg/mL, while also dose-dependently reducing fungal hyphal extension and altering colony morphology at higher concentrations^[1].
Blasticidin A (1 μM; 30 min) alters the proteome of Saccharomyces cerevisiae strain W303, reducing ribosomal protein abundance, increasing Gpd1p abundance, and promoting shifts to phosphorylated forms of key translational and chaperone proteins^[2].
Blasticidin A (1 μM; 30 min) does not alter transcription of ribosomal protein genes ASC1 and RPP0 but increases transcription of GPD1 in Saccharomyces cerevisiae strain W303^[2].
Blasticidin A (0.1-1 μM; 60 min) inhibits galactose-induced 6×His-LacZ protein synthesis in a dose-dependent manner in plasmid-transformed Saccharomyces cerevisiae strain W303^[2].
Blasticidin A potently and selectively inhibits aflatoxin production in Aspergillus parasiticus NRRL2999, with an IC₅₀ of 0.25 μM for aflatoxin production and an IC₅₀ of 1.6 μM for fungal growth, giving a selectivity ratio of 6.4^[2].
Blasticidin A (0.3 mM; 24-36 h) alters the proteome of Aspergillus flavus IMF47798, reducing abundances of aflatoxin biosynthetic enzymes after 36 h and reducing abundances of multiple metabolic and hypothetical proteins while increasing glutathione S-transferase abundances after both 24 h and 36 h^[2].
Blasticidin A selectively inhibits aflatoxin production in Aspergillus flavus IMF47798, with an IC₅₀ of 0.25 μM for aflatoxin production and an IC₅₀ of 1.6 μM for fungal growth^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1186.46

C₅₈H₁₀₇NO₂₃

100513-53-9

Solid

White to off-white

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Room temperature in continental US; may vary elsewhere.

• [1]. Sakuda S, et al. Blasticidin A as an inhibitor of aflatoxin production by Aspergillus parasiticus. J Antibiot (Tokyo). 2000;53(11):1265-1271.  [Content Brief]

  [2]. Yoshinari T, et al. Inhibitory activity of blasticidin A, a strong aflatoxin production inhibitor, on protein synthesis of yeast: selective inhibition of aflatoxin production by protein synthesis inhibitors. J Antibiot (Tokyo). 2010;63(6):309-314.  [Content Brief]