Cat. No.: HY-133968

24-Methylenecholesterol

(Synonyms: Ostreasterol)

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

24-Methylenecholesterol (Ostreasterol) is a regulator targeting acyl-CoA cholesterol acyltransferase (ACAT) with anti-aging and neuroprotective effects. 24-Methylenecholesterol mimics the effects of nerve growth factor (NGF), can extend yeast lifespan through an anti-oxidative stress mechanism, and exhibits neuroprotective activity in PC12 cells. 24-Methylenecholesterol can reduce intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels, activate anti-oxidative stress pathways (such as UTH1, SOD-related genes), and promote synaptic growth.

For research use only. We do not sell to patients.

Purity: 99.78%
CAS No.: 474-63-5
Formula: C₂₈H₄₆O
Molecular Weight:398.66

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

24-Methylenecholesterol Chemical Structure

CAS No.: 474-63-5

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Acyltransferase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Vero	IC₅₀	>128 μg/mL Compound: 1	Cytotoxicity against african green monkey Vero cells Cytotoxicity against african green monkey Vero cells	[PMID: 18818073]

In Vivo

24-Methylenecholesterol (0.1-1.0% dry feed concentration; dietary supplementation; once daily; 3 weeks) significantly upregulates the expression of head nutritional marker proteins (such as MRJP1, MRJP4-7), improves antioxidant enzyme activities and promoted larval development in the Apis mellifera model^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Apis mellifera worker bees (female, newly emerged, 80-100 mg body weight), nutritional physiology model^[2]
Dosage:

0.1% (S1), 0.25% (S2), 0.5% (S3), 0.75% (S4), 1.0% (S5) 24-Methylenecholesterol dissolved in acetone and mixed into synthetic diets (final acetone concentration <0.5%)
Administration:

Oral administration via synthetic diet, replaced daily, treatment for 3 weeks starting from newly emerged adulthood
Result:

Treatment with 24-Methylenecholesterol at 0.1-1.0% dry diet concentration significantly altered the head proteome of worker bees.
Compared to the control group, the mean relative abundances of major royal jelly proteins (MRJP1, MRJP4, MRJP5, MRJP7)-key nutritional markers involved in brood food synthesis-were upregulated by 20-35% in the S3-S5 groups (0.5-1.0% sterol).

Chemical Information

CAS No. 474-63-5
Appearance Solid
Molecular Weight 398.66
Formula C₂₈H₄₆O
Color White to off-white
SMILES

C=C(C(C)C)CC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
Synonyms

Ostreasterol
Structure Classification
- Steroids
Initial Source
- Plants
- other families
- Marine natural products
- Other marine organisms
- Animals
the larva of queen bees and from royal jelly
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (2)

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Journal Impact Factor

Plant J

Unveiling the inequivalent biochemical functions of OsSMO2-1 and OsSMO2-2 in rice phytosterol biosynthesis using a customized sterol standards mixture. [Abstract]

2025 Nov;124(3):e70559. PMID: 41194406
Biomed Rep

Chalinasterol attenuates ethanol-induced hepatic lipid accumulation by activating β-oxidation. [Abstract]

2026 Mar 9;24(5):57. PMID: 41859047

Solvent & Solubility

In Vitro:

Ethanol : 16.67 mg/mL (41.82 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5084 mL

12.5420 mL

25.0840 mL

5 mM

0.5017 mL

2.5084 mL

5.0168 mL

10 mM

0.2508 mL

1.2542 mL

2.5084 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Sun Y, et al. Sterols from Mytilidae show anti-aging and neuroprotective effects via anti-oxidative activity. Int J Mol Sci. 2014 Nov 25;15(12):21660-73. [Content Brief]

[2]. Chakrabarti P, et al. Changes in Honey Bee Head Proteome in Response to Dietary 24-Methylenecholesterol. Insects. 2020 Oct 29;11(11):743. [Content Brief]

[3]. Tabas I, et al. The reactivity of desmosterol and other shellfish- and xanthomatosis-associated sterols in the macrophage sterol esterification reaction. J Clin Invest. 1989 Dec;84(6):1713-21. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol	1 mM	2.5084 mL	12.5420 mL	25.0840 mL	62.7101 mL
	5 mM	0.5017 mL	2.5084 mL	5.0168 mL	12.5420 mL
	10 mM	0.2508 mL	1.2542 mL	2.5084 mL	6.2710 mL
	15 mM	0.1672 mL	0.8361 mL	1.6723 mL	4.1807 mL
	20 mM	0.1254 mL	0.6271 mL	1.2542 mL	3.1355 mL
	25 mM	0.1003 mL	0.5017 mL	1.0034 mL	2.5084 mL
	30 mM	0.0836 mL	0.4181 mL	0.8361 mL	2.0903 mL
	40 mM	0.0627 mL	0.3136 mL	0.6271 mL	1.5678 mL

Keywords

24-Methylenecholesterol

2 Cited Publications

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24-Methylenecholesterol

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Publications Citing Use of MedChemExpress (MCE) 24-Methylenecholesterol

All Acyltransferase Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

24-Methylenecholesterol Related Classifications

HY-133968 Related Classifications