JNJ-9676
Based on 1 Customer Validation
JNJ-9676 is an orally active Coronavirus M protein inhibitor and selective Sarbecovirus inhibitor. JNJ-9676 binds to the M protein dimer and forces the protein into an alternative conformational state with a compound-induced binding pocket. JNJ-9676 demonstrates in vitro nanomolar antiviral activity against SARS-CoV-2, SARS-CoV and Sarbecovirus strains from bat and pangolin zoonotic origin.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 3026520-19-1
- Formula: C28H21F2N5O2
- Molecular Weight:497.50
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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All DNA/RNA Synthesis Isoforms
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Biological Activity
JNJ-9676 exhibits in vitro antiviral activity against SARS-CoV-2 B1 with an EC50 ranging from 14 to 22 nM in a variety of cell types[1].
JNJ-9676 potently reduces SARS-CoV-2 (B1 variant) RNA production in primary human nasal epithelium air-liquid interface cultures with an EC50 of 94.0 nM and EC90 of 132.0 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Syrian golden hamsters (female, 8-10 weeks old, SARS-CoV-2 B1 strain intranasal infection)[1]
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Dosage:75 mg per kg; 25 mg per kg; 8.33 mg per kg
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Administration:p.o.; BID
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Result:Reduced lung viral RNA by 3.5
log10 copies per mg lung, infectious virus by 4 log10 TCiD50 per mg lung at 25 mg/kg.
Chemical Information
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CAS No. 3026520-19-1
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Appearance Solid
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Molecular Weight 497.50
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Formula C28H21F2N5O2
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Color White to off-white
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SMILES
N#CC1=CC=CC(NC(C2=C3N(N=C2)C[C@@H](N(C4=CC=C(C=C4)C(F)(C5=CC=CC=C5)F)C3=O)C)=O)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 200 mg/mL (402.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (10.05 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0101 mL | 10.0503 mL | 20.1005 mL | 50.2513 mL |
| 5 mM | 0.4020 mL | 2.0101 mL | 4.0201 mL | 10.0503 mL | |
| 10 mM | 0.2010 mL | 1.0050 mL | 2.0101 mL | 5.0251 mL | |
| 15 mM | 0.1340 mL | 0.6700 mL | 1.3400 mL | 3.3501 mL | |
| 20 mM | 0.1005 mL | 0.5025 mL | 1.0050 mL | 2.5126 mL | |
| 25 mM | 0.0804 mL | 0.4020 mL | 0.8040 mL | 2.0101 mL | |
| 30 mM | 0.0670 mL | 0.3350 mL | 0.6700 mL | 1.6750 mL | |
| 40 mM | 0.0503 mL | 0.2513 mL | 0.5025 mL | 1.2563 mL | |
| 50 mM | 0.0402 mL | 0.2010 mL | 0.4020 mL | 1.0050 mL | |
| 60 mM | 0.0335 mL | 0.1675 mL | 0.3350 mL | 0.8375 mL | |
| 80 mM | 0.0251 mL | 0.1256 mL | 0.2513 mL | 0.6281 mL | |
| 100 mM | 0.0201 mL | 0.1005 mL | 0.2010 mL | 0.5025 mL |
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