Cat. No.: HY-N0761A

trans-Isoferulic acid

(Synonyms: trans-3-Hydroxy-4-methoxycinnamic acid)

Customer Review

Based on 1 Customer Validation

trans-Isoferulic acid (trans-3-Hydroxy-4-methoxycinnamic acid) is an aromatic acid that exhibits anti-inflammatory activity. trans-Isoferulic acid dephosphorylates PI3K/Akt, suppresses LPS (HY-D1056)-induced NF-κB activation, enhances Nrf2 activity, downregulates iNOS and COX-2 expression, induces HO-1 expression, inhibits NO and PGE₂ production, and scavenges hydroperoxyl radical. trans-Isoferulic acid can be used for research of inflammatiory diseases.

For research use only. We do not sell to patients.

Purity: 99.93%
CAS No.: 25522-33-2
Formula: C₁₀H₁₀O₄
Molecular Weight:194.19

Storage:

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

trans-Isoferulic acid Chemical Structure

CAS No.: 25522-33-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

iNOS

COX-2

All NO Synthase Isoforms :

All Prostaglandin Receptor Isoforms :

All PI3K Isoforms :

All Akt Isoforms :

All NF-κB Isoforms :

All COX Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	>515 μM Compound: 10	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 12027739]
B16-BL6	EC₅₀	>515 μM Compound: 10	Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 12027739]
EA.hy 926	EC₅₀	29.3 μM Compound: 3-OH-4-OCH3-cinnamic acid	Cytoprotective activity against H2O2-induced cytotoxicity in human EAhy926 cells pre-incubated for 24 hrs before H2O2 challenge for 12 hrs by MTT assay Cytoprotective activity against H2O2-induced cytotoxicity in human EAhy926 cells pre-incubated for 24 hrs before H2O2 challenge for 12 hrs by MTT assay	10.1039/C4MD00022F
HeLa	EC₅₀	>515 μM Compound: 10	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay	[PMID: 12027739]
HeLa	IC₅₀	≥10 μM Compound: isoferulic acid	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 19278239]
HT-1080	EC₅₀	>515 μM Compound: 10	Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 12027739]
Oocyte	IC₅₀	13.5 μM Compound: 4-O-Me-Caf-COOH	Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique	[PMID: 23682762]
RAW264.7	IC₅₀	>50 μM Compound: 12	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method	[PMID: 22079762]

In Vitro

trans-Isoferulic acid (25-200 μM; 1 h pretreatment) does not reduce viability or induce apoptosis in BV2 microglial cells at concentrations ≤150 μM, but causes significant cytotoxicity at 200 μM^[1].
trans-Isoferulic acid (50-150 μM; 1 h pretreatment) dose-dependently inhibits LPS-induced NO production in BV2 microglial cells, with complete inhibition at 150 μM^[1].
trans-Isoferulic acid (50-150 μM; 1 h pretreatment) dose-dependently inhibits LPS-induced PGE₂ production in BV2 microglial cells, with complete inhibition at 150 μM^[1].
trans-Isoferulic acid (50-150 μM; 1 h pretreatment) dose-dependently suppresses LPS-induced iNOS and COX-2 mRNA and protein expression in BV2 microglial cells^[1].
trans-Isoferulic acid (150 μM; 1 h pretreatment) completely inhibits LPS-induced NF-κB DNA-binding activity in BV2 microglial cells^[1].
trans-Isoferulic acid (150 μM; 1 h pretreatment) suppresses LPS-induced phosphorylation of PI3K and Akt in BV2 microglial cells^[1].
trans-Isoferulic acid (50-150 μM; 0-24 h) time- and dose-dependently upregulates HO-1 mRNA and protein expression in BV2 microglial cells^[1].
trans-Isoferulic acid (50-150 μM; 1 h pretreatment) dose-dependently increases Nrf2 DNA-binding activity in BV2 microglial cells^[1].
trans-Isoferulic acid (150 μM; 1 h pretreatment) induced HO-1 expression and subsequent inhibition of LPS-induced NO and PGE₂ production in BV2 microglial cells are dependent on Nrf2 activation^[1].
trans-isoferulic acid scavenges HOO• radicals via pH-dependent mechanisms^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

BV2 microglial cells
Concentration:

25; 50; 75; 100; 150; 200 μM
Incubation Time:

24 h (with 1 h pre-incubation before LPS)
Result:

Showed no cytotoxicity at concentrations ≤150 μM, regardless of LPS treatment.
Detected a statistically significant reduction in cell viability at 200 μM.

ELISA Assay^[1]

Cell Line:

LPS-stimulated BV2 microglial cells
Concentration:

50; 100;150 μM
Incubation Time:

24 h (with 1 h pre-incubation before LPS)
Result:

Reduced LPS-induced PGE₂ levels in a dose-dependent manner.
Dose-dependently suppressed LPS-induced iNOS and COX-2 mRNA.

Real Time qPCR^[1]

Cell Line:

LPS-stimulated BV2 microglial cells
Concentration:

50; 100; 150 μM
Incubation Time:

3; 6; 9 h
Result:

Significantly decreased LPS-induced HO-1 mRNA levels in a dose-dependent manner.
Significantly decreased LPS-induced HO-1 mRNA levels in a time-dependent manner.

Chemical Information

CAS No. 25522-33-2
Appearance Solid
Molecular Weight 194.19
Formula C₁₀H₁₀O₄
Color White to light brown
SMILES

O=C(O)/C=C/C1=CC=C(OC)C(O)=C1
Synonyms

trans-3-Hydroxy-4-methoxycinnamic acid
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Plants
- Ranunculaceae
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (514.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.1496 mL

25.7480 mL

51.4960 mL

5 mM

1.0299 mL

5.1496 mL

10.2992 mL

10 mM

0.5150 mL

2.5748 mL

5.1496 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (12.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (12.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Dilshara MG, et al. Downregulation of NO and PGE2 in LPS-stimulated BV2 microglial cells by trans-isoferulic acid via suppression of PI3K/Akt-dependent NF-κB and activation of Nrf2-mediated HO-1. Int Immunopharmacol. 2014;18(1):203-211. [Content Brief]

[2]. Sun KH, et al. A new indole-type alkaloid from the roots of Clematis florida var. plena. Nat Prod Res. 2019 Oct;33(20):2925-2931. [Content Brief]

[3]. Kowalska-Baron A, et al. Theoretical Insight into Antioxidant Mechanisms of Trans-Isoferulic Acid in Aqueous Medium at Different pH. Int J Mol Sci. 2025;26(12):5615. Published 2025 Jun 11. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.1496 mL	25.7480 mL	51.4960 mL	128.7399 mL
	5 mM	1.0299 mL	5.1496 mL	10.2992 mL	25.7480 mL
	10 mM	0.5150 mL	2.5748 mL	5.1496 mL	12.8740 mL
	15 mM	0.3433 mL	1.7165 mL	3.4331 mL	8.5827 mL
	20 mM	0.2575 mL	1.2874 mL	2.5748 mL	6.4370 mL
	25 mM	0.2060 mL	1.0299 mL	2.0598 mL	5.1496 mL
	30 mM	0.1717 mL	0.8583 mL	1.7165 mL	4.2913 mL
	40 mM	0.1287 mL	0.6437 mL	1.2874 mL	3.2185 mL
	50 mM	0.1030 mL	0.5150 mL	1.0299 mL	2.5748 mL
	60 mM	0.0858 mL	0.4291 mL	0.8583 mL	2.1457 mL
	80 mM	0.0644 mL	0.3218 mL	0.6437 mL	1.6092 mL
	100 mM	0.0515 mL	0.2575 mL	0.5150 mL	1.2874 mL

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trans-Isoferulic acid

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All NO Synthase Isoforms

All Prostaglandin Receptor Isoforms

All PI3K Isoforms

All Akt Isoforms