3'-BPANeuGc
3'-BPANeuGc is a high-affinity selective Siglec-G ligand. Lipid nanoparticles surface-modified with antigens and 3'-BPANeuGc inhibit BCR signal transduction. 3'-BPANeuGc is applicable to antigen-specific tolerance research.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- CAS No.: 1596134-81-4
- Formule: C41H58N4O20
- Masse moléculaire:926.91
-
Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
-
Activité biologique
-
Chemical Information
- Pureté et documentation
- Références
-
Help & FAQs
Activité biologique
3'-BPANeuGc (1-4%; 40 min at 4°C) is a selective, high-affinity ligand for Siglec-G, as demonstrated by specific binding to Siglec-G-expressing BW5147 cells and murine splenic B cells, with no cross-reactivity to other murine siglecs[1].
3'-BPANeuGc (1-4%) when co-displayed with anti-IgM on liposomes substantially suppresses calcium flux in both murine peritoneal B1a cells and splenic B2 cells in a Siglec-G-dependent manner[1].
3'-BPANeuGc (1-4%; 3 min) when co-displayed with HEL on liposomes-mediated recruitment of Siglec-G to the BCR strongly dampens proximal and distal BCR signaling cascade components in splenic HEL-reactive MD4 B cells in an Shp-1-dependent manner[1].
3'-BPANeuGc (1-4%; 4 days) when co-displayed with HEL on liposomes inhibits proliferation and reduces survival of HEL-reactive MD4 B cells in a Siglec-G-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:splenic HEL-reactive MD4 B cells (wild-type and Shp-1-deficient)
-
Concentration:1-4% (in 5 μM liposomes containing HEL)
-
Incubation Time:3 min
-
Result:Suppressed phosphorylation or degradation of multiple BCR signaling components, including those involved in MAP kinase, Erk, NFkB, and Akt pathways, when co-displayed with HEL on liposomes, compared to liposomes displaying HEL alone.
Showed no inhibitory effect in Shp-1-deficient (Moth eaten) B cells.
-
Cell Line:HEL-reactive MD4 B cells (wild-type and Siglec-G-deficient)
-
Concentration:1-4% (in liposomes containing HEL)
-
Incubation Time:4 days
-
Result:Caused pronounced diminishment in proliferation and a significant reduction in live cell numbers when co-displayed with HEL on liposomes, compared to liposomes displaying HEL alone.
Showed no such effects in Siglec-G-deficient MD4 B cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:C57BL/6J (wild-type; Siglec-G-deficient; MD4 transgenic, IgMHEL-expressing)[1]
-
Dosage:0.5 mM total lipid concentration (delivered in 200 µl PBS)
-
Administration:i.p.; single dose; i.v.; single dose
-
Result:Showed greatly reduced anti-NP IgM and IgG titers compared to mice receiving NP-only liposomes.
Failed to produce anti-NP antibodies upon subsequent challenge with NP-only liposomes.
Chemical Information
-
CAS No. 1596134-81-4
-
Masse moléculaire 926.91
-
Formule C41H58N4O20
-
SMILES
OC([C@@]1(O[C@@]([C@@H]([C@H](C1)O)NC(CO)=O)([H])[C@H](O)[C@H](O)CNC(CC2=CC=C(C3=CC=CC=C3)C=C2)=O)O[C@@H]4[C@H]([C@@H](O[C@@H]([C@@H]4O)CO)O[C@@H]5[C@H](O[C@H]([C@@H]([C@H]5O)NC(C)=O)OCCN)CO)O)=O
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz