CU-CPT17e
Based on 3 publication(s) in Google Scholar
CU-CPT17e is a potent multi-Toll-like receptor (TLR) agonist that activates TLR3, TLR8, and TLR9.
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- Pureté: 98.67%
- CAS No.: 2109805-75-4
- Formule: C27H24N2O8
- Masse moléculaire:504.49
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) CU-CPT17e
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Activité biologique
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TLR3 |
TLR8 |
TLR9 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | EC50 |
>100 μM
Compound: 17e; CU-CPT17e
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Agonist activity at human TLR2 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR2 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
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[PMID: 28537730] |
| HEK293 | EC50 |
>100 μM
Compound: 17e; CU-CPT17e
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Agonist activity at human TLR4 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR4 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
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[PMID: 28537730] |
| HEK293 | EC50 |
>100 μM
Compound: 17e; CU-CPT17e
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Agonist activity at human TLR5 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR5 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
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[PMID: 28537730] |
| HEK293 | EC50 |
>100 μM
Compound: 17e; CU-CPT17e
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Agonist activity at human TLR7 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR7 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
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[PMID: 28537730] |
| HEK293 | EC50 |
13.45 μM
Compound: 17e; CU-CPT17e
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Agonist activity at human TLR8 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR8 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
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[PMID: 28537730] |
| HEK293 | EC50 |
4.8 μM
Compound: 17e; CU-CPT17e
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Agonist activity at human TLR3 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR3 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
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[PMID: 28537730] |
| HEK293 | EC50 |
5.66 μM
Compound: 17e; CU-CPT17e
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Agonist activity at human TLR9 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
Agonist activity at human TLR9 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay
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[PMID: 28537730] |
| HeLa | IC50 |
15.77 μM
Compound: 17e; CU-CPT17e
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Antiproliferative activity against human HeLa cells after 48 hrs by WST1 assay
Antiproliferative activity against human HeLa cells after 48 hrs by WST1 assay
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[PMID: 28537730] |
| HeLa | IC50 |
2.71 μM
Compound: 17e; CU-CPT17e
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Antiproliferative activity against human HeLa cells after 72 hrs by WST1 assay
Antiproliferative activity against human HeLa cells after 72 hrs by WST1 assay
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[PMID: 28537730] |
| HeLa | IC50 |
38.56 μM
Compound: 17e; CU-CPT17e
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Antiproliferative activity against human HeLa cells after 24 hrs by WST1 assay
Antiproliferative activity against human HeLa cells after 24 hrs by WST1 assay
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[PMID: 28537730] |
| HMEC | IC50 |
>100 μM
Compound: 17e; CU-CPT17e
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Antiproliferative activity against HMEC after 24 hrs by WST1 assay
Antiproliferative activity against HMEC after 24 hrs by WST1 assay
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[PMID: 28537730] |
| HMEC | IC50 |
>100 μM
Compound: 17e; CU-CPT17e
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Antiproliferative activity against HMEC after 48 hrs by WST1 assay
Antiproliferative activity against HMEC after 48 hrs by WST1 assay
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[PMID: 28537730] |
| HMEC | IC50 |
79.53 μM
Compound: 17e; CU-CPT17e
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Antiproliferative activity against HMEC after 72 hrs by WST1 assay
Antiproliferative activity against HMEC after 72 hrs by WST1 assay
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[PMID: 28537730] |
CU-CPT17e shows strong NF-κB activation in TLR3, TLR8 and TLR9 HEK293 cells with EC50 values of 4.80±0.73, 13.5±0.58 and 5.66±0.17 μM, respectively. CU-CPT17e significantly improves the activity with 13.9±0.9 fold of NF-κB activation and an EC50 value of 4.8±0.7 μM. CU-CPT17e inhibits the proliferation of HeLa cancer cells by triggering apoptosis and arresting the cell cycle at the S phase. The induction of apoptosis by CU-CPT17e in HeLa cells is investigated. HeLa cells are cultured with increasing concentrations of CU-CPT17e or poly I:C or blank control (DMSO) for 24 h. Treatment with CU-CPT17e for 24 h at different concentrations (10 to 40 μM) results in an elevation of apoptotic cell population ranging from 10% to 17%, which is more effective than poly I:C at 5 μg/mL. These results suggest that the antiproliferative activity of CU-CPT17e against HeLa cells might result from its ability to directly induce apoptosis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2109805-75-4
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Appearance Solid
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Masse moléculaire 504.49
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Formule C27H24N2O8
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Color Light yellow to yellow
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SMILES
O=[N+]([O-])C(C=C1)=CC=C1COC2=CC(C=CC3(CCOCC3)O4)=C4C=C2OCC5=CC=C([N+]([O-])=O)C=C5
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (3)
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Journal Impact Factor
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Most Recent
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Wound Repair Regen
Foamy macrophages potentially inhibit tuberculous wound healing by inhibiting the TLRs/NF-κB signalling pathway. [Abstract]2022 May;30(3):376-396. PMID: 35384137 -
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Solvant et solubilité
DMSO : 5 mg/mL (9.91 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
HeLa cells are seeded at a density of 3×105 cells/well in 6-well plates and allowed to attach for 24 h. After treatment of indicated concentrations of CU-CPT17e or poly I:C (5 μg/mL) for another 24 h, cells are harvested with 0.25% trypsin without EDTA and rinsed twice with PBS, then stained using a Annexin V-FITC apoptosis detection kit. Cells are analyzed with a BD Accuri C6 flow cytometer[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureté et documentation
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Fiche technique (276 KB)
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SDS (544 KB)
- English - EN (544 KB)
- Français - FR (544 KB)
- Deutsch - DE (544 KB)
- Norwegian - NO (544 KB)
- Español - ES (544 KB)
- Swedish - SV (544 KB)
- Italian - IT (544 KB)
- Korean - KR (544 KB)
- Portuguese - PT (544 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9822 mL | 9.9110 mL | 19.8220 mL | 49.5550 mL |
| 5 mM | 0.3964 mL | 1.9822 mL | 3.9644 mL | 9.9110 mL |
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