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Nile Red

(Synonyms: Nile Blue A oxazone; Phenoxazone 9)
129 Cited Publications
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Based on 129 publication(s) in Google Scholar

Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm.

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  • Pureté: 99.74%
  • CAS No.: 7385-67-3
  • Formule: C20H18N2O2
  • Masse moléculaire:318.37
  • Stockage:

    4°C, protect from light

    * In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

  • Nile Red Chemical Structure
    Nile Red Chemical Structure
  • Experimental Validation Results for Nile Red
  • Experimental Validation Results for Nile Red
  • Experimental Validation Results for Nile Red
  • Experimental Validation Results for Nile Red
  • Experimental Validation Results for Nile Red
  • Experimental Validation Results for Nile Red
  • Experimental Validation Results for Nile Red
  • Experimental Validation Results for Nile Red
  • Experimental Validation Results for Nile Red
  • Experimental Validation Results for Nile Red
CAS No.: 7385-67-3
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  • Nile Red Chemical Structure
    Nile Red Chemical Structure
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    Nile Red purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2023 Jan 5:938:175428. [Abstract]

    Customer Validation
     Hepatic lipid and FFA content were determined by Nile red staining of liver frozen sections.

  • 1/16

    Nile Red purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Oct 5;13(1):5871. [Abstract]

    Customer Validation
     Isolated oocytes are fixed with 4% paraformaldehyde for 1 hour at room temperature and washed 3 times with 1% BSA in PBS. Then, they are stained with 10 µg/ml Nile red at 4 °C overnight.

  • 1/16

    Nile Red purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 Jun 24;50(12):6953-6967. [Abstract]

    Customer Validation
     The wild-type or G4-mutant human hepatic adenocarcinoma HepG2 cells are stained with Nile Red reagent to visualize the lipids with an Olympus Fluoview FV1000 confocal microscope.

  • 1/16

    Nile Red purchased from MedChemExpress. Usage Cited in: Nano Today. 47 (2022) 101675

    Customer Validation
     Subcellular biodistribution of NileRed-labeled PFD Liposome nano-particles in 4T1 and BT-549 cells was visualized by confocal laser microscopy. Blue, DAPI; Green, WGA 488; Red, NileRed-labeled PFD Liposome.

  • 1/16

    Nile Red purchased from MedChemExpress. Usage Cited in: J Transl Med. 2022 May 14;20(1):222. [Abstract]

    Customer Validation
     Living cells are incubated with Nile Red (1 μM) for 15 min at 37 °C in dark. Then, cells are washed with HBSS/Ca/Mg and analyzed by fluorescence microscopy.

  • 1/16

    Nile Red purchased from MedChemExpress. Usage Cited in: EMBO J. 2022 Aug 16;41(16):e110439. [Abstract]

    Customer Validation
     Representative pictures of Nile red-stained human BAs differentiated from hESC-derived brown progenitors.

  • 1/16

    Nile Red purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Jan 1;11(5):2149-2169. [Abstract]

    Customer Validation
     Injected 5 μg of Nile Red lipid dye into the tail vein of mice to label triglycerides in the liver prior to intravital imaging.

  • 1/16

    Nile Red purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2021 Nov;54(11):e13134. [Abstract]

    Customer Validation
     For lipid droplet staining, fixed cells are stained for 30 minutes with 0.1 µg/mL Nile Red (MCE), and the nucleus is counterstained using DAPI.

  • 1/16

    Nile Red purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2021 May 31;22(11):5951. [Abstract]

    Customer Validation
     For lipid staining, fat bodies are submerged in Nile red solution at a final working concentration of 10 μg/mL in an acetone/water (1:9) mixture and visualized using a fluorescent microscope at Ex543/Em626 nm.

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    Nile Red purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Jan 13;11(1):240. [Abstract]

    Customer Validation
     αSMA, Bodipy and Nile Red staining of HSCs, data are representative of n = 3 biological independent samples. Scale bar, 50 μm.

  • Nile Red