Bimagrumab

Human IgG1 lambda

Immobilized ACVR2B Protein, Human can bind Bimagrumab. The EC₅₀ for this effect is 8.254 ng/mL.

Flow cytometric analysis of 10⁶ Jurkat cells with Bimagrumab (HY-P99355, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 lambda1 (HY-P99992, blue) was used as the isotype control.

Bimagrumab purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 1;16(1):5976. [Abstract]

Customer Validation
Representative microscopy images showing C2C12 myoblast differentiation in the presence of Myostatin, soluble DLK1 + Myostatin, Bimagrumab + Myostatin, or Bimagrumab + soluble DLK1 + Myostatin. Control cells were allowed to differentiate for 72h. Myostatin treatment (4 µg/ml) inhibits C2C12 myoblast differentiation into myotubes as determined by MyoHC staining. Bimagrumab (100 nM) and soluble DLK1 (2 μM) reverse the effect of myostatin on C2C12 differentiation, with no additive effect of adding soluble DLK1 to Bimagrumab treated cells. C2C12 cells were fixed with 4% PFA, immunostained with an anti-MyoHC antibody and an anti-mouse Alexa Fluor 488 secondary antibody. Nuclei were counterstained with Hoechst 33342. Nuclei represented with pseudo color (magenta). The images are representative of one out of two imaging wells per treatment from one experiment. The experiment was independently repeated two times. Scale bar, 100 μm.

Bimagrumab purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Sep 30;16(1):8654. [Abstract]

Customer Validation
Pancreatic tumor samples from chow (n = 6 mice per group) and diabetic (n = 9 mice per group) KPC tumor-bearing mice treated with IgG, bimagrumab, or bimagrumab+metformin. The results showed that two injections of bimagrumab (20 mg/kg, subcutaneously, once weekly) reduced tumor burden in diabetic mice.

Bimagrumab purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Sep 30;16(1):8654. [Abstract]

Customer Validation
Phosphorylated Smad2 levels and total Smad2 expression were detected via western blotting in KPC tumors from the chow and diabetic mice treated with IgG or bimagrumab (20 mg/kg, subcutaneous injection, once weekly for two weeks) (n=3 mice per group).

Bimagrumab purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Sep 30;16(1):8654. [Abstract]

Customer Validation
Representative images of picrosirius red staining (red) in tumors from chow and diabetic KPC tumor-bearing mice treated with IgG or bimagrumab (20 mg/kg, subcutaneous injection, once weekly for two weeks) (n = 6 mice per group).

Bimagrumab purchased from MedChemExpress. Usage Cited in: Mol Metab. 2024 Feb:80:101880. [Abstract]

Customer Validation
Bimagrumab (20 mg/kg, subcutaneous injection, once weekly for two weeks). Representative TA sections stained with H&E (20x magnification, scale bar = 100 um).