cis-VZ185
cis-VZ185 serves as a non-degradative control and inactive analog of VZ185 (HY-114322), a dual-target BRD7/BRD9 degrader PROTAC. cis-VZ185 exerts antiproliferative activity against epithelioid sarcoma cells only at high concentrations. cis-VZ185 induces morphological changes in osteosarcoma cells. cis-VZ185 can be used in studies related to epithelioid sarcoma.
(Pink: BRD9 and BRD7 ligand (HY-111905A); Blue: VHL ligand (HY-176302); Black: linker).
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- CAS No.: 2306193-98-4
- Formule: C53H67FN8O8S
- Masse moléculaire:995.21
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
In Vitro
cis-VZ185 (10 μM; 20 h) induces significant morphological changes in U2OS cells, with an induction rate of 80%[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2306193-98-4
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Masse moléculaire 995.21
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Formule C53H67FN8O8S
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SMILES
CN1C=C(C2=CC=NC=C2C1=O)C3=CC(OC)=C(CN4CCN(CCCCCOC5=C(CNC([C@H]6N(C([C@@H](NC(C7(F)CC7)=O)C(C)(C)C)=O)C[C@@H](O)C6)=O)C=CC(C8=C(N=CS8)C)=C5)CC4)C(OC)=C3
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
Références
[1]. Rasmussen SV, et al. Functional genomic analysis of epithelioid sarcoma reveals distinct proximal and distal subtype biology. Clinical and translational medicine. 2022 Jul;12(7):e961. [Content Brief]
[2]. Jiang M, et al. LIN28-Targeting Chromenopyrazoles and Tetrahydroquinolines Induced Cellular Morphological Changes and Showed High Biosimilarity with BRD PROTACs. ChemMedChem. 2025 Jan 02;20(1):e202400547. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
cis-VZ185 Related Classifications
HY-114322A Related Classifications
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