dNaMTP
dNaMTP is an unnatural deoxyribonucleoside triphosphate substrate that DNA polymerases use to replicate unnatural base pairs containing dNaM. dNaMTP enables replication, transcription, and translation of DNA with an expanded genetic alphabet in semi-synthetic organisms. dNaMTP is used in synthetic biology research such as unnatural base pair replication analysis, preparation of DNA with an expanded genetic alphabet, and general sequencing of DNA containing unnatural bases.
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- CAS No.: 1117893-11-4
- Formule: C16H21O13P3
- Masse moléculaire:514.25
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
In Vitro
dNaMTP supports the amplification and assembly of pINF containing dNaM paired opposite dTPT3 in cell-free PCR[2].
dNaMTP can participate in gap insertion, labeled ligation, and PCR amplification reactions of KRAS-related DNA substrates under specific conditions, and some unnatural base sites cause premature termination in Sanger sequencing[3].
dNaMTP can be used together with dTPT3TP for PCR amplification to prepare IL-2 and pylT gene fragments containing the XY unnatural base pair, which are then assembled via Golden Gate to obtain an expression plasmid carrying this unnatural base pair[4].
dNaMTP and dTPT3TP are added together to the culture medium to maintain the stability of the XY unnatural base pair in E. coli during electroporation-based construction of the AzK-substituted IL-2 expression strain[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1117893-11-4
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Masse moléculaire 514.25
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Formule C16H21O13P3
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SMILES
OP(OP(OP(O)(OC[C@@H]1[C@@H](O)C[C@H](C2=CC3=C(C=CC=C3)C=C2OC)O1)=O)(O)=O)(O)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Wu J, et al. Engineered polymerase-mediated efficient synthesis of site-specifically functionalized RNA and 2’-modified RNA oligonucleotides via genetic alphabet expansion. Nature Communications. 2026 Aug 13. [Content Brief]
[2]. Malyshev DA, et al. A semi-synthetic organism with an expanded genetic alphabet. Nature. 2014 May 15;509(7500):385-8. [Content Brief]
[3]. Riedl J, et al. Identification of DNA lesions using a third base pair for amplification and nanopore sequencing. Nature communications. 2015 Nov 06;6:8807. [Content Brief]
[4]. Ptacin JL, et al. An engineered IL-2 reprogrammed for anti-tumor therapy using a semi-synthetic organism. Nature communications. 2021 Aug 09;12(1):4785. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
dNaMTP Related Classifications
HY-157263 Related Classifications
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