Cat. No.: HY-126010

Dooku1

Name: Dooku1
Brand: MedChemExpress
SKU: HY-126010
Price: 110.00 USD
Availability: InStock
Rating: 5 (16 reviews)

16 Cited Publications

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Based on 16 publication(s) in Google Scholar

Dooku1 is a reversibly Yoda1 antagonist with IC₅₀ value of 1.3 μM and 1.5 μM for 2 μM Yoda1-induced Ca²⁺ entry HEK 293 cells and HUVECs, respectively. Dooku1 can disrupt Yoda1-induced Piezo1 channel activity and inhibit Yoda1-induced relaxation of aorta. Dooku1 can be used for vascular physiology and disease research.

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Pureté: 99.90%
CAS No.: 2253744-54-4
Formule: C₁₃H₉Cl₂N₃OS
Masse moléculaire:326.20

Stockage:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Dooku1 Chemical Structure

CAS No.: 2253744-54-4

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (16)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

In Vitro

Dooku1 (10 μM, 300 s) has selectivity for Piezo1 channels in HEK 293 and CHO cells.^[1].
Dooku1 (10 μM, 140 s) has no effect on constitutive Piezo1 channel activity in Piezo1 T-RE_x cells^[1].
Dooku1 (10 μM, 40-60 s) inhibits endogenous Yoda1-activated channels in HUVECs and Piezo1 T-RE_x cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

HUVECs, Piezo1 T-RE_x cells
Concentration:

10 μM
Incubation Time:

40-60 s
Result:

Had a concentration-dependent inhibitory effect against Yoda1-induced Ca²⁺ entry in HUVECs, acting with an IC₅₀ of 1.49 μM.
Increased potency in HUVECs with an EC₅₀ of 0.23 μM, compared with 2.51 μM in Piezo1 T-RE_x cells.

In Vivo

Dooku1 (10 μM incubate 20 min) selectively inhibits Yoda1-induced relaxation of aorta of wild-type male C57BL/6 mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

wild-type male C57BL/6 mice’s aortic rings^[1]
Dosage:

10 μM
Administration:

20 min
Result:

Suppressed the Yoda1-induced relaxation.

Chemical Information

CAS No. 2253744-54-4
Appearance Solid
Masse moléculaire 326.20
Formule C₁₃H₉Cl₂N₃OS
Color White to off-white
SMILES

ClC1=C(C(Cl)=CC=C1)CSC2=NN=C(C3=CC=CN3)O2
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Publications (16)

All Bio/Physico-chemical AssayCell Imaging/StainingRT-PCRIF

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Journal Impact Factor

Nat Commun

Polypeptide sono-adjuvant for ultrasound-activatable regulation of innate immunity and cancer vaccination therapy. [Abstract]

2025 Dec 16;17(1):272. PMID: 41402324

Dooku1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 16;17(1):272. [Abstract]

The addition of Dooku-1 markedly abrogated Ca2+ influx induced by US stimulation and significantly inhibited US-triggered active caspase-1 release in DCs.
Neuron

Microglial Piezo1 senses Aβ fibril stiffness to restrict Alzheimer's disease. [Abstract]

2023 Jan 4;111(1):15-29.e8. PMID: 36368316
Sci Adv

Mechano-gradients drive morphogen-noise correction to ensure robust patterning. [Abstract]

2024 Nov 15;10(46):eadp2357. PMID: 39546611
Mater Today Bio

Piezo1 mediated scaffold-free rapid generation of self-mineralized bone organoids via activating Wnt signaling. [Abstract]

2025 Dec 3:36:102620. PMID: 41476751

Dooku1 purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Dec 3:36:102620. [Abstract]

Pre-treatment with Dooku1 slowed the rate of spheroid formation.

Dooku1 purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Dec 3:36:102620. [Abstract]

IF staining confirmed the on-target activity of Dooku1, as it significantly suppressed the Yoda1 (0.5 μM)-induced upregulation of Piezo1 itself
Mater Today Bio

Magnetically guided neurite outgrowth modulated by PIEZO1 in hiPSC-derived retinal ganglion cells. [Abstract]

2025 Oct 21:35:102446. PMID: 41209706
Hum Reprod Open

Impaired PIEZO1 function drives uterine hypercontractility in adenomyosis-associated dysmenorrhea. [Abstract]

2026 Feb 17;2026(2):hoag013. PMID: 41836349
ACS Appl Mater Interfaces

Shear-Stress Initiates Signal Two of NLRP3 Inflammasome Activation in LPS-Primed Macrophages through Piezo1. [Abstract]

2025 Feb 5;17(5):7363-7376. PMID: 39836089

Dooku1 purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2025 Feb 5;17(5):7363-7376. [Abstract]

Dooku1 inhibits shear-stress-induced NLRP3 inflammasome activation in iBMDMs. (A) A representative imaging panel is shown above for ASC−CFP expressed iBMDMs primed LPS for 4 h followed by treatments of 10 or 50 μM Dooku1 for 2 h and 50 dyn/cm2 shear stress treatment for 30 min. LPS only controls were also performed according to aforementioned time points. NucRed stains living cell nuclei in blue, ASC−CFP specks indicating inflammasome activation are shown in cyan, and propidium iodide (PI) shows dead cell nuclei in red.
Cell Rep

Mechanical force receptor Piezo1 regulates TH9 cell differentiation. [Abstract]

2025 Jan 28;44(1):115136. PMID: 39932192

Dooku1 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Jan 28;44(1):115136. [Abstract]

The pharmacological inhibition of Piezo1 (by Dooku1 (2 μM) and GsMTx4) in mouse T cells reduced IL-9 production and altered SIRT3, SDHA, and HIF1α expression.
Br J Pharmacol

Targeting the tyrosine kinase Src in endothelium attenuates inflammation and atherogenesis induced by disturbed flow. [Abstract]

2024 Aug 8. PMID: 39117589
Appl Mater Today

27, June 2022, 101465.
Cell Mol Life Sci

The transcription factor RBPJ is required for inflammatory macrophage activation in thoracic aortic dissection by mediating mechanotransduction-induced glycolysis. [Abstract]

2025 Oct 28;82(1):370. PMID: 41148245
Life Sci

Regulatory role of Piezo1 channel in endothelium-dependent hyperpolarization-mediated vasorelaxation of small resistance vessels and its anti-inflammatory action. [Abstract]

2024 Jan 1:336:122326. PMID: 38056769
Eur J Pharmacol

Activation of Piezo1 channels enhances spontaneous contractions of isolated human bladder strips via acetylcholine release from the mucosa. [Abstract]

2024 Nov 15:983:176954. PMID: 39237075
Am J Pathol

Fibrotic matrix induces mesenchymal transformation of epithelial cells in oral submucous fibrosis. [Abstract]

2023 Sep;193(9):1208-1222. PMID: 37328100
Curr Med Sci

High Hydrostatic Pressure Exacerbates Bladder Fibrosis through Activating Piezo1. [Abstract]

2024 Aug;44(4):718-725. PMID: 38926331
bioRxiv

2024 Mar 11.

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (306.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0656 mL

15.3280 mL

30.6560 mL

5 mM

0.6131 mL

3.0656 mL

6.1312 mL

10 mM

0.3066 mL

1.5328 mL

3.0656 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (6.38 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (276 KB)
SDS (394 KB)
- English - EN (394 KB)
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- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Evans EL, et al. Yoda1 analogue (Dooku1) which antagonizes Yoda1-evoked activation of Piezo1 and aortic relaxation. Br J Pharmacol. 2018 May;175(10):1744-1759. [Content Brief]

[2]. Evans EL, et al. Yoda1 analogue (Dooku1) which antagonizes Yoda1-evoked activation of Piezo1 and aortic relaxation. Br J Pharmacol. 2018 May;175(10):1744-1759. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0656 mL	15.3280 mL	30.6560 mL	76.6401 mL
	5 mM	0.6131 mL	3.0656 mL	6.1312 mL	15.3280 mL
	10 mM	0.3066 mL	1.5328 mL	3.0656 mL	7.6640 mL
	15 mM	0.2044 mL	1.0219 mL	2.0437 mL	5.1093 mL
	20 mM	0.1533 mL	0.7664 mL	1.5328 mL	3.8320 mL
	25 mM	0.1226 mL	0.6131 mL	1.2262 mL	3.0656 mL
	30 mM	0.1022 mL	0.5109 mL	1.0219 mL	2.5547 mL
	40 mM	0.0766 mL	0.3832 mL	0.7664 mL	1.9160 mL
	50 mM	0.0613 mL	0.3066 mL	0.6131 mL	1.5328 mL
	60 mM	0.0511 mL	0.2555 mL	0.5109 mL	1.2773 mL
	80 mM	0.0383 mL	0.1916 mL	0.3832 mL	0.9580 mL
	100 mM	0.0307 mL	0.1533 mL	0.3066 mL	0.7664 mL

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Keywords

Dooku1

16 Cited Publications

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Dooku1

Yoda 1

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Dimethyl sulfoxide, meets analytical specification of Ch.P.

TAK-243

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2-Aminoethyl diphenylborinate

Enzalutamide

XAV-939

Publications Citing Use of MedChemExpress (MCE) Dooku1

Activité biologique

Chemical Information

Publications (16)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

Dooku1

Yoda 1

GsMTx4

Jedi2

MG-132

Dimethyl sulfoxide, meets analytical specification of Ch.P.

TAK-243

Thapsigargin

L-NAME hydrochloride

Phorbol 12-myristate 13-acetate

2-Aminoethyl diphenylborinate

Enzalutamide

XAV-939

Publications Citing Use of MedChemExpress (MCE) Dooku1

Activité biologique

Chemical Information

Publications (16)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Dooku1 Related Classifications

HY-126010 Related Classifications

Bioz