M5N36
Based on 1 Customer Validation
M5N36 is a potent and selective Cdc25C inhibitor with IC50 values of 0.15, 0.19, 0.06 µM for Cdc25A, Cdc25B, Cdc25C, respectively. M5N36 shows anti-proliferative activity and increases the expression of p-CDK1 and p-CDK2.
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- Pureté: 98.00%
- CAS No.: 2832887-40-6
- Formule: C20H16ClN5O3
- Masse moléculaire:409.83
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Metabolism/Protease Compound Library
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Anti-Cancer Compound Library
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High-Throughput Bioactive Compound Library
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Activité biologique
IC50: 0.15 µM (Cdc25A); 0.19 µM (Cdc25B); 0.06 µM (Cdc25C)[1]
M5N36 (5, 10 µM; 24 h) increases the expression of p-CDK1 and p-CDK2 in MDA-MB-231 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Eca-109, HepG2, MCF-TAM, MDA-MB-231, Raji, SGC-7901, SW-620, HUVEC cells
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Concentration:
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Incubation Time:
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Result:Showed anti-proliferative activity with IC50s of 2.88, 3.57, 10.00, 2.00, 1.20, 6.03, 3.39, 2.40 µM for Eca-109, HepG2, MCF-TAM, MDA-MB-231, Raji, SGC-7901, SW-620, HUVEC cells, respectively.
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Cell Line:MDA-MB-231 cells
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Concentration:5, 10 µM
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Incubation Time:24 h
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Result:Increased the expression of Cdc25A, Cdc25B, increased the expression of p-CDK1 and p-CDK2.
Chemical Information
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CAS No. 2832887-40-6
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Appearance Solid
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Masse moléculaire 409.83
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Formule C20H16ClN5O3
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Color Orange to red
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SMILES
O=C1C(Cl)=C(C(C2=NC=CC=C21)=O)NC(C3=CN(N=N3)C4=CC=C(C=C4)CO)C
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 25 mg/mL (61.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.10 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.10 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4400 mL | 12.2002 mL | 24.4004 mL | 61.0009 mL |
| 5 mM | 0.4880 mL | 2.4400 mL | 4.8801 mL | 12.2002 mL | |
| 10 mM | 0.2440 mL | 1.2200 mL | 2.4400 mL | 6.1001 mL | |
| 15 mM | 0.1627 mL | 0.8133 mL | 1.6267 mL | 4.0667 mL | |
| 20 mM | 0.1220 mL | 0.6100 mL | 1.2200 mL | 3.0500 mL | |
| 25 mM | 0.0976 mL | 0.4880 mL | 0.9760 mL | 2.4400 mL | |
| 30 mM | 0.0813 mL | 0.4067 mL | 0.8133 mL | 2.0334 mL | |
| 40 mM | 0.0610 mL | 0.3050 mL | 0.6100 mL | 1.5250 mL | |
| 50 mM | 0.0488 mL | 0.2440 mL | 0.4880 mL | 1.2200 mL | |
| 60 mM | 0.0407 mL | 0.2033 mL | 0.4067 mL | 1.0167 mL |
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