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Cat. No.: HY-N21878

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Muraymycin D2

Muraymycin D2 is a peptidyl nucleoside natural product derived from Streptomyces spp., possessing competitive translocase I (MraY) inhibitory activity and antibacterial activity. Muraymycin D2 competitively binds to the nucleotide-binding pocket of the MraY substrate via its nucleoside moiety, and upon binding, induces a conformational change in MraY, blocking lipid I synthesis and inhibiting peptidoglycan biosynthesis. Muraymycin D2 exhibits a K_i of 7.6 nM against Bacillus subtilis and an IC₅₀ of 0.39 nM against Staphylococcus aureus. Muraymycin D2 shows antibacterial activity against Escherichia coli and is capable of breaking persistence in chlamydial infection. Muraymycin D2 can be used for research on bacterial infection-related studies, such as chlamydial infection.

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CAS No.: 474328-38-6
Formule: C₃₇H₆₁N₁₁O₁₆
Masse moléculaire:915.94

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Muraymycin D2 Chemical Structure

CAS No.: 474328-38-6

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- Cellular Effect
- In Vitro
Chemical Information
Pureté et documentation
Références
Calculators
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Classifications
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Activité biologique

Description

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Cellular Effect

Cell Line	Type	Value	Description	References
HEp-2	MIC	128 μg/mL	Minimal inhibitory concentration of Muraymycin D2 against C. trachomatis D/UW-3/CX in HEp-2 cells, inducing formation of large aberrant bodies, with compound added at 2 h post infection and effects analyzed at 30 h post infection by fluorescence microscopy. Minimal inhibitory concentration of Muraymycin D2 against C. trachomatis D/UW-3/CX in HEp-2 cells, inducing formation of large aberrant bodies, with compound added at 2 h post infection and effects analyzed at 30 h post infection by fluorescence microscopy.	antibiotics13050421

In Vitro

Bacterial InfectionOthers

Bacterial Infection

Muraymycin D2 competitively inhibits Bacillus subtilis MraY with a K_i of 7.6 nM^[1].
Muraymycin D2 (MRY D2) induces enlarged abnormal cells in C. trachomatis D/UW-3/CX, with an MIC of 128 µg/mL^[2].
Muraymycin D2 (0.05-0.5 µM; 90 min) inhibits the enzymatic activity of purified C. pneumoniae MraY^[2].
Muraymycin D2 (128 µg/mL; 18 h) eradicates persistent C. trachomatis D/UW-3/CX cells in PEN G-induced infection; when applied at the mid-stage, it reduces inclusions in active C. trachomatis D/UW-3/CX infection^[2].

Others

Muraymycin D2 binds to A. aeolicus MraY, inducing significant conformational changes and specific binding interactions^[1].
Muraymycin D2 exhibits non-Michaelis-Menten kinetics and substrate inhibition with Mur29, giving K_m = 41 μM and k_cat = 4.0 min⁻¹; it also exhibits non-Michaelis-Menten kinetics and substrate inhibition when reacted with Mur28, with K_m = 170 μM and k_cat = 5.2 min^−1[3].
Muraymycin D2 is regioselectively phosphorylated by the Mg·ATP-dependent 3''-phosphotransferase Mur28 to generate D2-PHOS; the phosphorylated muraymycin D2 exhibits significantly reduced inhibitory activity against S. aureus MraY^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[2]

Cell Line:

HEp-2 cells infected with C. trachomatis D/UW-3/CX
Concentration:

128 µg/mL
Incubation Time:

18 h (added at 12 hpi, analyzed at 30 hpi)
Result:

Resulted in a 70% reduction in the number of inclusions.\nObserved a 75% reduction in inclusions, of which 83% contained EBs/RBs and 8% had a mixed phenotype.

Related Protocols :

Chemical Information

CAS No. 474328-38-6
Masse moléculaire 915.94
Formule C₃₇H₆₁N₁₁O₁₆
SMILES

NC[C@H]([C@H]([C@H]1O)O)O[C@H]1O[C@H]([C@@]2([H])O[C@@H](N3C(NC(C=C3)=O)=O)[C@@H]([C@@H]2O)O)[C@@H](C(O)=O)NCCCNC([C@H](CC(C)C)NC([C@H]([C@@]4([H])NC(N)=NCC4)NC(N[C@H](C(O)=O)C(C)C)=O)=O)=O
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Microorganism
Streptomyces spp.
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Instruction de manipulation (2659 KB)

Références

[1]. Bugg TDH, et al. Mechanism of action of nucleoside antibacterial natural product antibiotics. The Journal of antibiotics. 2019 Dec;72(12):865-876.

[2]. Löckener I, et al. The MraY Inhibitor Muraymycin D2 and Its Derivatives Induce Enlarged Cells in Obligate Intracellular Chlamydia and Wolbachia and Break the Persistence Phenotype in Chlamydia. Antibiotics, 2024, 13: 421.

[3]. Cui Z, et al. Self-Resistance during Muraymycin Biosynthesis: a Complementary Nucleotidyltransferase and Phosphotransferase with Identical Modification Sites and Distinct Temporal Order. Antimicrobial agents and chemotherapy. 2018 Jul;62(7):e00193-18.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Activité biologique
- Description
- In Vitro
Chemical Information
Pureté et documentation
Références

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Muraymycin D2

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