TPCK-treated Trypsin
Based on 1 publication(s) in Google Scholar
TPCK-treated Trypsin is a trypsin whose activity is inhibited by tosyl phenylalanyl chloromethyl ketone. TPCK-treated Trypsin reduces autolysis and nonspecific proteolysis during experiments, exhibits stability in storage and handling. TPCK-treated trypsin can be used in proteomics research. TPCK-treated Trypsin renders the virus hemagglutinin active, which allows multicycle replication of the virus. TPCK-treated Trypsin can be used for the study of influenza virus.
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- CAS No.: 9002-07-7
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) TPCK-treated Trypsin
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Activité biologique
Product Information This product is derived from bovine pancreas. After chromatographic purification, it is treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit the activity of proteases. The theoretical molecular weight is 23.3 kDa, and the optimal pH is 7.5-8.5. It can be used for protein analysis, such as protein mass spectrometry, sequencing, and peptide mapping analysis. Instructions (for reference only) This product can be directly used for protein enzymatic cleavage. The recommended enzymatic cleavage ratio is 1:50-1:100 (w/w); Enzymatic cleavage overnight at 37°C.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.4.21.4
≥180 U/mg protein
One Unit hydrolyzes one micromole of TAME per minute at 25°C, pH 8.2, in the presence of 0.01M calcium. (One TAME unit = 19.2 USP/NF units = 57.5 BAEE units.)
Chemical Information
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CAS No. 9002-07-7
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Appearance Solid
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Color White to off-white
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SMILES
[TPCK-treated Trypsin]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
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Nat Commun
Dipeptidylpeptidase 4 inhibition attenuates gestational pathologies via immune homeostasis restoration in the pulmonary-uterine axis. [Abstract]2026 Feb 17;17(1):2851. PMID: 41702893
Solvant et solubilité
H2O : 100 mg/mL (Need ultrasonic)
Pureté et documentation
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Fiche technique (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Shuford CM, et al., Cheaper, faster, simpler trypsin digestion for high-throughput targeted protein quantification. J Mass Spectrom Adv Clin Lab. 2023 Nov 23;30:74-82. [Content Brief]
[2]. Cuevas F, et al. An In Vitro Microneutralization Assay for Influenza Virus Serology. Curr Protoc. 2022 Jul;2(7):e465. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
TPCK-treated Trypsin Related Classifications
HY-129047C Related Classifications
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