YE6144
Based on 2 publication(s) in Google Scholar
YE6144 free base is a selective prototypical interferon regulatory factor 5 (IRF5) inhibitor. YE6144 free base suppresses the disease course and is especially effective in remission maintenance in a mouse model of systemic lupus erythematosus (SLE). YE6144 free base can be used for SLE research.
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- Pureté: 96.47%
- CAS No.: 3065657-02-2
- Formule: C21H27ClFN7O
- Masse moléculaire:447.94
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) YE6144
More
Activité biologique
IRF5[1]
YE6144 (1 or 3 μM; 30 min) inhibits the phosphorylation of IRF5 in both human PBMCs and mouse splenocytes[1].
YE6144 (0-10 μM; 30 min) inhibits the production of type I IFNs with an IC50 of approximately 0.09 μM in human HC PBMCs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human HC PBMCs and mouse WT splenocytes
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Concentration:1 μM (PBMCs) and 3 μM (splenocytes)
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Incubation Time:30 min
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Result:Inhibited the phosphorylation of IRF5.
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Cell Line:Mouse WT splenocytes
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Concentration:3 μM
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Incubation Time:30 min
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Result:Induction of type I IFN genes, Ifnb1 and Ifna stimulated by TLR7 ligands or TLR9 ligands was remarkably weakened.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NZB/W F1 mouse model of systemic lupus erythematosus (SLE)[1]
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Dosage:40.0 mg/kg
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Administration:s.c., once
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Result:Suppressed the exacerbation of autoantibody production.
Splenomegaly and renal dysfunction were also suppressed by the treatment after disease onset.
Chemical Information
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CAS No. 3065657-02-2
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Appearance Solid
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Masse moléculaire 447.94
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Formule C21H27ClFN7O
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Color Orange to red
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SMILES
CC[C@@H](CNC1=CC2=C(NN=C2/C=C/C3=CC=C(N4CCOCC4)N=N3)C=C1F)N.[H]Cl
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
Solvant et solubilité
DMSO : 70 mg/mL (156.27 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 10 mg/mL (22.32 mM); Clear solution
This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 10 mg/mL (22.32 mM); Clear solution
This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2324 mL | 11.1622 mL | 22.3244 mL | 55.8110 mL |
| 5 mM | 0.4465 mL | 2.2324 mL | 4.4649 mL | 11.1622 mL | |
| 10 mM | 0.2232 mL | 1.1162 mL | 2.2324 mL | 5.5811 mL | |
| 15 mM | 0.1488 mL | 0.7441 mL | 1.4883 mL | 3.7207 mL | |
| 20 mM | 0.1116 mL | 0.5581 mL | 1.1162 mL | 2.7906 mL | |
| 25 mM | 0.0893 mL | 0.4465 mL | 0.8930 mL | 2.2324 mL | |
| 30 mM | 0.0744 mL | 0.3721 mL | 0.7441 mL | 1.8604 mL | |
| 40 mM | 0.0558 mL | 0.2791 mL | 0.5581 mL | 1.3953 mL | |
| 50 mM | 0.0446 mL | 0.2232 mL | 0.4465 mL | 1.1162 mL | |
| 60 mM | 0.0372 mL | 0.1860 mL | 0.3721 mL | 0.9302 mL | |
| 80 mM | 0.0279 mL | 0.1395 mL | 0.2791 mL | 0.6976 mL | |
| 100 mM | 0.0223 mL | 0.1116 mL | 0.2232 mL | 0.5581 mL |
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