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CAA-0225

CAA-0225 is a tissue protease L inhibitor that inhibits rat liver tissue protease L with a IC₅₀ value of 1.9 nM. CAA-0225 can participate in the degradation of autophagosome membrane markers LC3-II and GABARAP (HY-P72639), improve cardiac function in mice with reperfusion injury, and kill and eliminate Trypanosoma brucei parasites^[1][2][3].

For research use only. We do not sell to patients.

CAS No.: 244072-26-2
Formula: C₂₈H₂₉N₃O₅
Molecular Weight:487.55

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

CAA-0225 Chemical Structure

CAS No.: 244072-26-2

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Sample (0.1mg)

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

cathepsin L

All Cathepsin Isoforms :

In Vitro

CAA-0225 (10 mg/mL, 4 h) acts as a probe for autophagic protein hydrolysis by specifically inhibiting the involvement of protease L in the degradation of autophagosome membrane markers LC3-II and GABARAP^[1].
CAA-0225 (0-100 μM, 48 h) kills Trypanosoma brucei parasite by inhibiting tissue protease L^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1].

Cell Line:

HeLa、Huh-7 cells
Concentration:

10 mg/mL
Incubation Time:

4 h
Result:

Inhibited the degradation of microtubule associated protein IA/IB light chain 3-II (LC3-II) and gamma aminobutyric acid (A) receptor associated protein (GABARAP)-II

Cell Proliferation Assay^[3].

Cell Line:

Bloodstream forms of T. brucei clone 427–221a
Concentration:

0-100 μM
Incubation Time:

48 h
Result:

Inhibited the growth of trypanosomes in a dose-dependent manner with GI₅₀ values of 0.33 μM, respectively

In Vivo

CAA-0225 (0.25 mg, i.v.) improves cardiac function and reduces infarct size by inhibiting the release of cardiac cathepsin L in a mouse model of reperfusion after myocardial infarction^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Model of reperfusion injury involved male C57Bl/6 mice aged 9–12 weeks^[2].
Dosage:

0.25 mg
Administration:

Intravenous injection (i.v.)
Result:

Reduced infarct size, improved systolic and diastolic cardiac function.

Chemical Information

CAS No. 244072-26-2
Molecular Weight 487.55
Formula C₂₈H₂₉N₃O₅
SMILES

O=C([C@H]1O[C@@H]1C(N[C@@H](CC2=CC=CC=C2)C(NCC3=CC=CC=C3)=O)=O)NCCC4=CC=C(O)C=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Characterization of CAA0225, a Novel Inhibitor Specific for Cathepsin L, as a Probe for Autophagic Proteolysis

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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