Eragidomide
Based on 7 publication(s) in Google Scholar
Eragidomide (CC-90009) is a first-in-class GSPT1-selective cereblon (CRBN) E3 ligase modulator, acts as a molecular glue. Eragidomide coopts the CRL4CRBN to selectively target GSPT1 for ubiquitination and proteasomal degradation.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 1860875-51-9
- Formula: C22H18ClF2N3O4
- Molecular Weight:461.85
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Eragidomide
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
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WB
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Cell Proliferation/Viability Assay
Biological Activity
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Cereblon |
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Cell Line
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Type | Value | Description | References |
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| Bone marrow cell | EC50 |
6 nM
Compound: 16; CC-90009
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Antiproliferative activity in bone marrow cells derived from AML patients assessed as cell growth inhibition
Antiproliferative activity in bone marrow cells derived from AML patients assessed as cell growth inhibition
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[PMID: 33591756] |
| K562 | IC50 |
>40 μM
Compound: CC-90009
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Antiproliferative activity against human K562 cells incubated for 48 hrs by MTS assay
Antiproliferative activity against human K562 cells incubated for 48 hrs by MTS assay
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[PMID: 38795517] |
| MV4-11 | EC50 |
0.062 μM
Compound: 5; CC-90009
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Antiproliferative activity against human MV4-11 cells assessed as reduction in cell viability measured after 3 days by CellTiter-Glo assay
Antiproliferative activity against human MV4-11 cells assessed as reduction in cell viability measured after 3 days by CellTiter-Glo assay
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[PMID: 34042448] |
| OCI-AML2 | IC50 |
0.01 μM
Compound: CC-90009
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Antiproliferative activity against human OCI-AML2 cells incubated for 48 hrs by MTS assay
Antiproliferative activity against human OCI-AML2 cells incubated for 48 hrs by MTS assay
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[PMID: 38795517] |
| RS4-11 | IC50 |
0.1 nM
Compound: CC-90009
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Antiproliferative activity against human RS4-11 cells assessed as reduction in cell viability measured after 72 hrs by Alamar blue assay
Antiproliferative activity against human RS4-11 cells assessed as reduction in cell viability measured after 72 hrs by Alamar blue assay
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[PMID: 37390512] |
| U-266 | IC50 |
0.04 μM
Compound: CC-90009
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Antiproliferative activity against human U-266 cells incubated for 48 hrs by MTS assay
Antiproliferative activity against human U-266 cells incubated for 48 hrs by MTS assay
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[PMID: 38795517] |
| U-937 | IC50 |
<0.032 μM
Compound: CC-90009
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Antiproliferative activity against human U-937 cells incubated for 48 hrs by MTS assay
Antiproliferative activity against human U-937 cells incubated for 48 hrs by MTS assay
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[PMID: 38795517] |
Depletion of GSPT1 by Eragidomide rapidly induces acute myeloid leukemia (AML) apoptosis, reducing leukemia engraftment and leukemia stem cells (LSCs) in large-scale primary patient xenografting of 35 independent AML samples. Eragidomide activity is mediated by multiple layers of signaling networks and pathways within AML blasts and LSCs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 1860875-51-9
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Appearance Solid
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Molecular Weight 461.85
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Formula C22H18ClF2N3O4
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Color Off-white to light yellow
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SMILES
O=C(NCC1=CC2=C(C(N(C(CC3)C(NC3=O)=O)C2)=O)C=C1)C(F)(F)C4=CC=C(Cl)C=C4
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Synonyms
CC-90009
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (7)
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Journal Impact Factor
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Most Recent
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Cancer Res
Single-Cell Lineage Tracing Uncovers Resistance Signatures and Sensitizing Strategies to FLT3 Inhibitors in Acute Myeloid Leukemia. [Abstract]2025 Nov 21:10.1158/0008-5472.CAN-24-3753. PMID: 41270153
Eragidomide purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Nov 21:10.1158/0008-5472.CAN-24-3753. [Abstract]
After treating MOLM-13 cells with DMSO or a specified concentration of CC-90009 for 24 hours, Western blot analysis was performed on the GSPT1 protein level in the cells.
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Nat Chem Biol
2023 Mar;19(3):323-333. PMID: 36329119
Eragidomide purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2023 Mar;19(3):323-333. [Abstract]
Dose-resolved, normalized viability after 3 d treatment with CC-90009 in RKO CRBN-/- cells with over-expression of CRBNWT, CRBNE377K, CRBNN351D and CRBNH397D.
Eragidomide purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2023 Mar;19(3):323-333. [Abstract]
Protein levels in RKO CRBN-/- cells with over-expression of CRBNWT, CRBNN351D, CRBNH397D or CRBNH57D treated with DMSO, CC-90009 (50 nM, 6 h), dBET6 (15 nM, 2 h), dBET57 (240 nM, 2 h) or THAL-SNS-032 (200 nM, 2 h).
Eragidomide purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2023 Mar;19(3):323-333. [Abstract]
Depiction of clonogenic assays via crystal violet staining. Cells were treated for 10 days at EC90 of the degrader (30 nM dBET6, 60 nM CC-90009).
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Leukemia
Targeting of IRAK4 and GSPT1 enhances therapeutic efficacy in AML via c-Myc destabilization. [Abstract]2025 Sep;39(9):2163-2173. PMID: 40670672
Eragidomide purchased from MedChemExpress. Usage Cited in: Leukemia. 2025 Sep;39(9):2163-2173. [Abstract]
Cell viability was determined in the indicated cell lines cultured with CA-4948 (10 µM) or vehicle for 2 weeks and then treated with CC-90009 or vehicle (n = 4).
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Pharmaceutics
CC-90009, a Cereblon E3 Ligase Modulator, Exhibits Antiviral Efficacy Against JEV In Vitro and In Vivo via Targeted Degradation of GSPT1 and Viral NS5 Protein. [Abstract]2025 Nov 27;17(12):1524. PMID: 41471039 -
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Solvent & Solubility
DMSO : 100 mg/mL (216.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.50 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.50 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Christine Surka, et al. CC-90009, a novel cereblon E3 ligase modulator, targets acute myeloid leukemia blasts and leukemia stem cells. Blood. 2021 Feb 4;137(5):661-677. [Content Brief]
[2]. Joshua D Hansen, et al. CC-90009: A Cereblon E3 Ligase Modulating Drug That Promotes Selective Degradation of GSPT1 for the Treatment of Acute Myeloid Leukemia. J Med Chem. 2021 Feb 25;64(4):1835-1843. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.1652 mL | 10.8260 mL | 21.6521 mL | 54.1301 mL |
| 5 mM | 0.4330 mL | 2.1652 mL | 4.3304 mL | 10.8260 mL | |
| 10 mM | 0.2165 mL | 1.0826 mL | 2.1652 mL | 5.4130 mL | |
| 15 mM | 0.1443 mL | 0.7217 mL | 1.4435 mL | 3.6087 mL | |
| 20 mM | 0.1083 mL | 0.5413 mL | 1.0826 mL | 2.7065 mL | |
| 25 mM | 0.0866 mL | 0.4330 mL | 0.8661 mL | 2.1652 mL | |
| 30 mM | 0.0722 mL | 0.3609 mL | 0.7217 mL | 1.8043 mL | |
| 40 mM | 0.0541 mL | 0.2707 mL | 0.5413 mL | 1.3533 mL | |
| 50 mM | 0.0433 mL | 0.2165 mL | 0.4330 mL | 1.0826 mL | |
| 60 mM | 0.0361 mL | 0.1804 mL | 0.3609 mL | 0.9022 mL | |
| 80 mM | 0.0271 mL | 0.1353 mL | 0.2707 mL | 0.6766 mL | |
| 100 mM | 0.0217 mL | 0.1083 mL | 0.2165 mL | 0.5413 mL |
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