Eragidomide (Synonyms: CC-90009)

Name: Eragidomide
Brand: MedChemExpress
SKU: HY-130800
Rating: 5.0 (7 reviews)

Cat. No.: HY-130800 Purity: 99.93%: Data Sheet
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Eragidomide (CC-90009) is a first-in-class GSPT1-selective cereblon (CRBN) E3 ligase modulator, acts as a molecular glue. Eragidomide coopts the CRL4^CRBN to selectively target GSPT1 for ubiquitination and proteasomal degradation.

For research use only. We do not sell to patients.

CAS No. : 1860875-51-9

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 7 publication(s) in Google Scholar

7 Publications Citing Use of MCE Eragidomide

•bioRxiv. 2024 October 25.

Cell Proliferation/Viability Assay

: Eragidomide purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Nov 21:10.1158/0008-5472.CAN-24-3753. [Abstract]; After treating MOLM-13 cells with DMSO or a specified concentration of CC-90009 for 24 hours, Western blot analysis was performed on the GSPT1 protein level in the cells.

: Eragidomide purchased from MedChemExpress. Usage Cited in: Leukemia. 2025 Sep;39(9):2163-2173. [Abstract]; Cell viability was determined in the indicated cell lines cultured with CA-4948 (10 µM) or vehicle for 2 weeks and then treated with CC-90009 or vehicle (n = 4).

: Eragidomide purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2023 Mar;19(3):323-333. [Abstract]; Dose-resolved, normalized viability after 3 d treatment with CC-90009 in RKO CRBN-/- cells with over-expression of CRBNWT, CRBNE377K, CRBNN351D and CRBNH397D.

: Eragidomide purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2023 Mar;19(3):323-333. [Abstract]; Protein levels in RKO CRBN-/- cells with over-expression of CRBNWT, CRBNN351D, CRBNH397D or CRBNH57D treated with DMSO, CC-90009 (50 nM, 6 h), dBET6 (15 nM, 2 h), dBET57 (240 nM, 2 h) or THAL-SNS-032 (200 nM, 2 h).

: Eragidomide purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2023 Mar;19(3):323-333. [Abstract]; Depiction of clonogenic assays via crystal violet staining. Cells were treated for 10 days at EC90 of the degrader (30 nM dBET6, 60 nM CC-90009).

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•Related Screening Libraries:

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View All Ligands for E3 Ligase Isoform Specific Products:

View All Isoforms

von Hippel-Lindau (VHL) MDM2 Cereblon IAP KLHDC2 Fbxo3 RNF4 DCAF11 DCAF1 UHRF1 TRIM21 NEURL1B

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Cereblon

Cellular Effect

Cell Line	Type	Value	Description	References
Bone marrow cell	EC₅₀	6 nM Compound: 16; CC-90009	Antiproliferative activity in bone marrow cells derived from AML patients assessed as cell growth inhibition Antiproliferative activity in bone marrow cells derived from AML patients assessed as cell growth inhibition	[PMID: 33591756]
K562	IC₅₀	> 40 μM Compound: CC-90009	Antiproliferative activity against human K562 cells incubated for 48 hrs by MTS assay Antiproliferative activity against human K562 cells incubated for 48 hrs by MTS assay	[PMID: 38795517]
MV4-11	EC₅₀	0.062 μM Compound: 5; CC-90009	Antiproliferative activity against human MV4-11 cells assessed as reduction in cell viability measured after 3 days by CellTiter-Glo assay Antiproliferative activity against human MV4-11 cells assessed as reduction in cell viability measured after 3 days by CellTiter-Glo assay	[PMID: 34042448]
OCI-AML2	IC₅₀	0.01 μM Compound: CC-90009	Antiproliferative activity against human OCI-AML2 cells incubated for 48 hrs by MTS assay Antiproliferative activity against human OCI-AML2 cells incubated for 48 hrs by MTS assay	[PMID: 38795517]
RS4-11	IC₅₀	0.1 nM Compound: CC-90009	Antiproliferative activity against human RS4-11 cells assessed as reduction in cell viability measured after 72 hrs by Alamar blue assay Antiproliferative activity against human RS4-11 cells assessed as reduction in cell viability measured after 72 hrs by Alamar blue assay	[PMID: 37390512]
U-266	IC₅₀	0.04 μM Compound: CC-90009	Antiproliferative activity against human U-266 cells incubated for 48 hrs by MTS assay Antiproliferative activity against human U-266 cells incubated for 48 hrs by MTS assay	[PMID: 38795517]
U-937	IC₅₀	< 0.032 μM Compound: CC-90009	Antiproliferative activity against human U-937 cells incubated for 48 hrs by MTS assay Antiproliferative activity against human U-937 cells incubated for 48 hrs by MTS assay	[PMID: 38795517]

In Vitro

Depletion of GSPT1 by Eragidomide rapidly induces acute myeloid leukemia (AML) apoptosis, reducing leukemia engraftment and leukemia stem cells (LSCs) in large-scale primary patient xenografting of 35 independent AML samples. Eragidomide activity is mediated by multiple layers of signaling networks and pathways within AML blasts and LSCs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

461.85

Formula

C₂₂H₁₈ClF₂N₃O₄

CAS No.

1860875-51-9

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(NCC1=CC2=C(C(N(C(CC3)C(NC3=O)=O)C2)=O)C=C1)C(F)(F)C4=CC=C(Cl)C=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (216.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1652 mL	10.8260 mL	21.6521 mL
5 mM	0.4330 mL	2.1652 mL	4.3304 mL
10 mM	0.2165 mL	1.0826 mL	2.1652 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (246 KB) HNMR (295 KB) RP-HPLC (246 KB) MS (180 KB)

Handling Instructions (2659 KB)

References

[1]. Christine Surka, et al. CC-90009, a novel cereblon E3 ligase modulator, targets acute myeloid leukemia blasts and leukemia stem cells. Blood. 2021 Feb 4;137(5):661-677. [Content Brief]

[2]. Joshua D Hansen, et al. CC-90009: A Cereblon E3 Ligase Modulating Drug That Promotes Selective Degradation of GSPT1 for the Treatment of Acute Myeloid Leukemia. J Med Chem. 2021 Feb 25;64(4):1835-1843. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1652 mL	10.8260 mL	21.6521 mL	54.1301 mL
	5 mM	0.4330 mL	2.1652 mL	4.3304 mL	10.8260 mL
	10 mM	0.2165 mL	1.0826 mL	2.1652 mL	5.4130 mL
	15 mM	0.1443 mL	0.7217 mL	1.4435 mL	3.6087 mL
	20 mM	0.1083 mL	0.5413 mL	1.0826 mL	2.7065 mL
	25 mM	0.0866 mL	0.4330 mL	0.8661 mL	2.1652 mL
	30 mM	0.0722 mL	0.3609 mL	0.7217 mL	1.8043 mL
	40 mM	0.0541 mL	0.2707 mL	0.5413 mL	1.3533 mL
	50 mM	0.0433 mL	0.2165 mL	0.4330 mL	1.0826 mL
	60 mM	0.0361 mL	0.1804 mL	0.3609 mL	0.9022 mL
	80 mM	0.0271 mL	0.1353 mL	0.2707 mL	0.6766 mL
	100 mM	0.0217 mL	0.1083 mL	0.2165 mL	0.5413 mL

Eragidomide Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Eragidomide (Synonyms: CC-90009)

Customer Review

Eragidomide Related Antibodies

7 Publications Citing Use of MCE Eragidomide

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Ligands for E3 Ligase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Eragidomide Related Antibodies

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Complete Stock Solution Preparation Table

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