Nile Red

Nile Red purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Oct 5;13(1):5871. [Abstract]

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Isolated oocytes are fixed with 4% paraformaldehyde for 1 hour at room temperature and washed 3 times with 1% BSA in PBS. Then, they are stained with 10 µg/ml Nile red at 4 °C overnight.

Nile Red purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 Jun 24;50(12):6953-6967. [Abstract]

Customer Validation
The wild-type or G4-mutant human hepatic adenocarcinoma HepG2 cells are stained with Nile Red reagent to visualize the lipids with an Olympus Fluoview FV1000 confocal microscope.

Nile Red purchased from MedChemExpress. Usage Cited in: Nano Today. 47 (2022) 101675

Customer Validation
Subcellular biodistribution of NileRed-labeled PFD Liposome nano-particles in 4T1 and BT-549 cells was visualized by confocal laser microscopy. Blue, DAPI; Green, WGA 488; Red, NileRed-labeled PFD Liposome.

Nile Red purchased from MedChemExpress. Usage Cited in: EMBO J. 2022 Aug 16;41(16):e110439. [Abstract]

Customer Validation
Representative pictures of Nile red-stained human BAs differentiated from hESC-derived brown progenitors.

Nile Red purchased from MedChemExpress. Usage Cited in: J Transl Med. 2022 May 14;20(1):222. [Abstract]

Customer Validation
Living cells are incubated with Nile Red (1 μM) for 15 min at 37 °C in dark. Then, cells are washed with HBSS/Ca/Mg and analyzed by fluorescence microscopy.

Nile Red purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2023 Jan 5:938:175428. [Abstract]

Customer Validation
Hepatic lipid and FFA content were determined by Nile red staining of liver frozen sections.

Nile Red purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Jan 1;11(5):2149-2169. [Abstract]

Customer Validation
Injected 5 μg of Nile Red lipid dye into the tail vein of mice to label triglycerides in the liver prior to intravital imaging.

Nile Red purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2021 Nov;54(11):e13134. [Abstract]

Customer Validation
For lipid droplet staining, fixed cells are stained for 30 minutes with 0.1 µg/mL Nile Red (MCE), and the nucleus is counterstained using DAPI.

Nile Red purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2021 May 31;22(11):5951. [Abstract]

Customer Validation
For lipid staining, fat bodies are submerged in Nile red solution at a final working concentration of 10 μg/mL in an acetone/water (1:9) mixture and visualized using a fluorescent microscope at Ex543/Em626 nm.

Nile Red purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Jan 13;11(1):240.  [Abstract]

Customer Validation
αSMA, Bodipy and Nile Red staining of HSCs, data are representative of n = 3 biological independent samples. Scale bar, 50 μm.