Azonafide-PEABA
Azonafide-PEABA is a cytotoxic agent moiety.
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- CAS. Nr.: 2752193-52-3
- Formel: C32H33N5O4
- Molecular Weight:551.64
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
Traditional Cytotoxic Agents |
Chemical Information
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CAS. Nr. 2752193-52-3
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Molecular Weight 551.64
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Formel C32H33N5O4
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SMILES
O=C(N1CCN2CCN(CCNC(C3=CC=C(N)C=C3)=O)CC2)C4=C5C(C(OC)=CC=C5C1=O)=CC6=CC=CC=C46
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)