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Art. -Nr.: HY-124616

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CBB3001

CBB3001 is an inhibitor of LSD1/KDM1A. CBB3001 exerts its effect by inhibiting Histone Demethylase activity, with an IC₅₀ of 21.25 μM. It downregulates the expression of pluripotency-related proteins and genes such as SOX2 and OCT4, and induces cell differentiation, growth arrest, and apoptosis. CBB3001 can be used for research on testicular germ cell tumors, teratomas, and embryonal carcinomas.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr.: 1639358-50-1
Formel: C₁₉H₂₇NO₅
Molecular Weight:349.43

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

CBB3001 Chemical Structure

CAS. Nr.: 1639358-50-1

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

^[2]

KDM1/LSD1

SOX2

OCT4

Alle Histone Demethylase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
PA-1	IC50	20 μM	Growth inhibition against human ovarian teratocarcinoma PA-1 cells. Growth inhibition against human ovarian teratocarcinoma PA-1 cells.	30765888
F9	IC50	20 μM	Growth inhibition against mouse embryonal carcinoma F9 cells. Growth inhibition against mouse embryonal carcinoma F9 cells.	30765888

In Vitro

CBB3001 (1-100 μM) potently inhibits the demethylase activity of purified recombinant GST-LSD1 protein in vitro, with an IC₅₀ of 21.25 μM^[2].
CBB3001 (20 μM; 16 h) inhibits LSD1 histone demethylase activity in human colorectal cancer HCT116 cells and human ovarian teratocarcinoma PA-1 cells, thereby increasing the levels of monomethylated and dimethylated H3K4, but does not affect the level of trimethylated H3K4^[2].
CBB3001 (20-40 μM; 16-30 h) downregulates the expression of the pluripotent stem cell proteins SOX2 and OCT4 in human ovarian teratocarcinoma PA-1 cells and mouse embryonal carcinoma F9 cells^[2].
CBB3001 (20 μM; 20 h) reduces EZH2 protein levels in wild-type mouse embryonic fibroblasts^[4].
CBB3001 (10 h treatment) reduces the protein levels of mSWI/SNF complex subunits in CAGGCre-ER/LSD1^fl/fl mouse embryonic fibroblasts (MEFs) within 10 h^[5].
CBB3001 (1-40 μM; 12-30 h) selectively inhibits the growth of human ovarian teratocarcinoma PA-1 cells and mouse embryonal carcinoma F9 cells, but exerts no significant effect on the growth of human colorectal cancer HCT116 cells or mouse fibroblast NIH3T3 cells^[2].
CBB3001 (20-40 μM) potently inhibits the growth of human ovarian teratocarcinoma (PA-1) cells and mouse embryonal carcinoma (F9) cells, with an IC₅₀ of approximately 20 μM. It also reduces the expression of pluripotency markers and exerts no effect on non-pluripotent tumor cells^[3].
CBB3001, a histone demethylase inhibitor, inhibits cell growth, downregulates pluripotency, and upregulates differentiation in testicular germ cell tumor cell lines^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

human colorectal carcinoma HCT116 cells, human ovarian teratocarcinoma PA-1 cells
Concentration:

20 μM
Incubation Time:

16 h
Result:

Increased levels of mono-methylated H3K4 and di-methylated H3K4 in both HCT116 and PA-1 cells.
Left the level of trimethylated H3K4 unchanged in both HCT116 and PA-1 cells.

Cell Proliferation Assay^[2]

Cell Line:

human ovarian teratocarcinoma PA-1 cells, human colorectal carcinoma HCT116 cells, mouse embryonic carcinoma F9 cells, mouse fibroblast NIH3T3 cells
Concentration:

1, 5, 10, 20, 40 μM (PA-1 and HCT116 cells); 20, 40 μM (F9 and NIH3T3 cells)
Incubation Time:

16 hours (PA-1 and HCT116 cells); 30 hours (F9 and NIH3T3 cells)
Result:

Potently inhibited the growth of PA-1 cells at concentrations between 5-40 μM, with reduced relative cell percentages observed at each tested concentration.
Did not significantly affect the growth of HCT116 cells even at concentrations up to 40 μM.
Inhibited the growth of F9 cells at 20 and 40 μM.
Had no significant effect on NIH3T3 cell growth at 20 and 40 μM.

Western Blot Analysis^[2]

Cell Line:

human ovarian teratocarcinoma PA-1 cells, mouse embryonic carcinoma F9 cells
Concentration:

20, 40 μM (PA-1 cells); 40 μM (F9 cells)
Incubation Time:

16 hours (PA-1 cells); 30 hours (F9 cells)
Result:

Downregulated SOX2 and OCT4 protein levels in PA-1 cells compared to control-treated cells.
Downregulated SOX2 and OCT4 protein levels in F9 cells compared to control-treated cells.

Cell Proliferation Assay^[2]

Cell Line:

human ovarian teratocarcinoma PA-1 cells
Concentration:

20, 40 μM
Incubation Time:

24 hours initial incubation, followed by 24 hours recovery in fresh medium
Result:

Did not reverse growth inhibitory effects on PA-1 cells after removing the compound and allowing 24 hours of recovery in fresh medium.
Left reduced relative cell percentages remaining similar to levels immediately after treatment.

Western Blot Analysis^[4]

Cell Line:

wild-type mouse embryonic fibroblasts (MEFs)
Concentration:

20 μM
Incubation Time:

20 h
Result:

Reduced EZH2 protein level significantly.
Left KDM1A protein levels unchanged.

Chemical Information

CAS. Nr. 1639358-50-1
Molecular Weight 349.43
Formel C₁₉H₂₇NO₅
SMILES

N(C(OC(C)(C)C)=O)[C@H]1[C@@H](C1)C2=CC=C(OC(OC(C)(C)C)=O)C=C2
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Tong D, et al. The emerging roles of histone demethylases in cancers. Cancer metastasis reviews. 2024 Jun;43(2):795-821.

[2]. Hoang N, et al. New histone demethylase LSD1 inhibitor selectively targets teratocarcinoma and embryonic carcinoma cells. Bioorganic & medicinal chemistry. 2018 May 01;26(8):1523-1537.

[3]. Jostes S, et al. Epigenetic drugs and their molecular targets in testicular germ cell tumours. Nature reviews. Urology. 2019 Apr;16(4):245-259.

[4]. Guo P, et al. A methylation-phosphorylation switch controls EZH2 stability and hematopoiesis. eLife. 2024 Feb 12;13:e86168.

[5]. Guo P, et al. The assembly of mammalian SWI/SNF chromatin remodeling complexes is regulated by lysine-methylation dependent proteolysis. Nature communications. 2022 Nov 05;13(1):6696.

[6]. Marroncelli N, et al. Is Human Chorionic Gonadotropin a Reliable Marker for Testicular Germ Cell Tumor? New Perspectives for a More Accurate Diagnosis. Cancers. 2025 Jul 21;17(14):2409.

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

MOQ

Minimum order quantity

100 mg

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise

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CBB3001

Alle Histone Demethylase Isoform-spezifische Produkte anzeigen