Art. -Nr.: HY-43643

DC-5163

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

DC-5163 is a potent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) inhibitor with an IC₅₀ of 176.3 nM and a K_d of 3.192 μM. DC-5163 can inhibit glycolysis pathway partially. DC-5163 also selectively inhibits cancer cell proliferation and induces apoptosis.

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Reinheit: 99.30%
CAS. Nr.: 897771-47-0
Formel: C₁₈H₂₀ClN₃OS
Molecular Weight:361.89

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

DC-5163 Chemical Structure

CAS. Nr.: 897771-47-0

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50: 176.3 nM (Glyceraldehyde-3-phosphate dehydrogenase (GAPDH))^[1]
Kd: 3.192 μM (GAPDH)^[1]

In Vitro

DC-5163 (100 μM; 48 hours; MDA-MB-231 cells) treatment clearly induces the apoptosis of MDA-MB-231 cells. DC-5163 also significantly reduces glucose uptake, lactate production, and 18F-FDG uptake rate compared to controls in MDA-MB-231cells^[1].
DC-5163 treatment at 25 μM inhibits GAPDH activity in BT-549 cells, MCF7 cells, HCT116 cells, MDA-MB-231 cells and A549 cells after 48 hours^[1].
DC-5163 inhibits MDA-MB-231 cell proliferation with an IC₅₀ of 99.22 μM at 48 hours and 52.09 μM at 72 hours^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

100 μM
Incubation Time:

48 hours
Result:

Clearly induced the apoptosis of MDA-MB-231 cells.

Chemical Information

CAS. Nr. 897771-47-0
Appearance Solid
Molecular Weight 361.89
Formel C₁₈H₂₀ClN₃OS
Color White to off-white
SMILES

S=C(N1CCN(CC2=CC=CC=C2)C1)NC3=CC=C(OC)C(Cl)=C3
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Journal Impact Factor

Cancer Cell Int

The therapeutic effect of a novel GAPDH inhibitor in mouse model of breast cancer and efficacy monitoring by molecular imaging. [Abstract]

2024 May 29;24(1):188. PMID: 38811918

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 300 mg/mL (828.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7633 mL

13.8164 mL

27.6327 mL

5 mM

0.5527 mL

2.7633 mL

5.5265 mL

10 mM

0.2763 mL

1.3816 mL

2.7633 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 7.5 mg/mL (20.72 mM); Clear solution

This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Li T, et al. Discovery of novel glyceraldehyde-3-phosphate dehydrogenase inhibitor via docking-based virtual screening. Bioorg Chem. 2020 Mar;96:103620. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7633 mL	13.8164 mL	27.6327 mL	69.0818 mL
	5 mM	0.5527 mL	2.7633 mL	5.5265 mL	13.8164 mL
	10 mM	0.2763 mL	1.3816 mL	2.7633 mL	6.9082 mL
	15 mM	0.1842 mL	0.9211 mL	1.8422 mL	4.6055 mL
	20 mM	0.1382 mL	0.6908 mL	1.3816 mL	3.4541 mL
	25 mM	0.1105 mL	0.5527 mL	1.1053 mL	2.7633 mL
	30 mM	0.0921 mL	0.4605 mL	0.9211 mL	2.3027 mL
	40 mM	0.0691 mL	0.3454 mL	0.6908 mL	1.7270 mL
	50 mM	0.0553 mL	0.2763 mL	0.5527 mL	1.3816 mL
	60 mM	0.0461 mL	0.2303 mL	0.4605 mL	1.1514 mL
	80 mM	0.0345 mL	0.1727 mL	0.3454 mL	0.8635 mL
	100 mM	0.0276 mL	0.1382 mL	0.2763 mL	0.6908 mL

Keywords

DC-5163

1 Cited Publications

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DC-5163

Apoptosis Compound Library

Anti-Cancer Compound Library

Glycolysis Compound Library

Anti-Cancer Metabolism Compound Library

Glucose Metabolism Compound Library

Highly Selective Activators Library

Cell Death Library

Extracellular Vesicles (EVs) Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) DC-5163

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

DC-5163

Apoptosis Compound Library

Anti-Cancer Compound Library

Glycolysis Compound Library

Anti-Cancer Metabolism Compound Library

Glucose Metabolism Compound Library

Highly Selective Activators Library

Cell Death Library

Extracellular Vesicles (EVs) Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) DC-5163

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

DC-5163 Related Classifications

HY-43643 Related Classifications