Art. -Nr.: HY-136144

FIDAS-5

9 Cited Publications

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Based on 9 publication(s) in Google Scholar

FIDAS-5 is a potent and orally active methionine S-adenosyltransferase 2A (MAT2A) inhibitor with an IC₅₀ of 2.1 μM. FIDAS-5 effectively competes against S-adenosylmethionine (SAM) for MAT2A binding. FIDAS-5 has anticancer activities.

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Reinheit: 98.46%
CAS. Nr.: 1391934-98-7
Formel: C₁₅H₁₃ClFN
Molecular Weight:261.72

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years

* The compound is unstable in solutions, freshly prepared is recommended.

FIDAS-5 Chemical Structure

CAS. Nr.: 1391934-98-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (9)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50: 2.1 μM (Methionine S-adenosyltransferase 2A (MAT2A))^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	49.8 μM Compound: 2b	Inhibition of human ERG over-expressed in HEK293 cell membrane by [3H]dofetilide binding assay Inhibition of human ERG over-expressed in HEK293 cell membrane by [3H]dofetilide binding assay	[PMID: 24950374]
LS174T	IC₅₀	7.6 nM Compound: 2b	Antiproliferative activity against human LS 174T cells by cell viability analyzer Antiproliferative activity against human LS 174T cells by cell viability analyzer	[PMID: 24950374]

In Vitro

FIDAS-5 (3 μM; 7 days; LS174T cells) treatment significantly inhibits the proliferation of LS174T cells^[1].
FIDAS-5 (3 μM) treatment inhibits the expression of c-Myc and cyclinD1 in LS174T CRC cells. And FIDAS-5 induces the expression of cell cycle inhibitor, p21^WAF1/CIP1^[1].
FIDAS-5 (3 μM; 36 h) treatment reduces the levels of both S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in LS174T cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

LS174T colorectal cancer (CRC) cells
Concentration:

3 μM
Incubation Time:

7 days
Result:

Significantly inhibited the proliferation of LS174T cells.

In Vivo

FIDAS-5 (20 mg/kg; oral gavage; daily; for two weeks; athymic nude mice) treatment significantly inhibits the growth of xenograft tumors, with minimal difference in body weight^[1].
Mice are treated with FIDAS-5 (20 mg/kg) for 1 week. The liver SAM levels are significantly reduced^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

16 athymic nude mice injected with HT29 CRC cells^[1]
Dosage:

20 mg/kg
Administration:

Oral gavage; daily; for two weeks
Result:

Significantly inhibited the growth of xenograft tumors.

Chemical Information

CAS. Nr. 1391934-98-7
Appearance Solid
Molecular Weight 261.72
Formel C₁₅H₁₃ClFN
Color Light yellow to yellow
SMILES

CNC1=CC=C(/C=C/C2=C(F)C=CC=C2Cl)C=C1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years

* The compound is unstable in solutions, freshly prepared is recommended.

Publications (9)

All In Vivo Efficacy StudyWBHistological Imaging/Staining

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Journal Impact Factor

Cell Metab

Dietary methionine mitigates immune-mediated damage by enhancing renal clearance of cytokines. [Abstract]

2026 Feb 3;38(2):298-315.e6. PMID: 41576934

FIDAS-5 purchased from MedChemExpress. Usage Cited in: Cell Metab. 2026 Feb 3;38(2):298-315.e6. [Abstract]

Survival of infected mice treated with either vehicle or FIDAS-5 (40 mg/kg).
Nat Metab

Multi-omics profiling of cachexia-targeted tissues reveals a spatio-temporally coordinated response to cancer. [Abstract]

2026 Jan;8(1):237-259. PMID: 41540255

FIDAS-5 purchased from MedChemExpress. Usage Cited in: Nat Metab. 2026 Jan;8(1):237-259. [Abstract]

C2C12 myotubes were treated with 100 ng/mL recombinant IL-6 and varying doses of FIDAS-5 for 48 hours. Activation of the rIL-6 signaling cascade was verified by assessing pY705-STAT3 protein levels.
Nat Metab

Vitamin B12 is a limiting factor for induced cellular plasticity and tissue repair. [Abstract]

2023 Nov;5(11):1911-1930. PMID: 37973897

FIDAS-5 purchased from MedChemExpress. Usage Cited in: Nat Metab. 2023 Nov;5(11):1911-1930. [Abstract]

On day 14 after DSS treatment, the recovery of colonic homeostasis in the FIDAS-5 (MAT2Ai: 20 mg/kg) group was evaluated using H&E staining.
Nat Neurosci

The mitochondrial unfolded protein response in human microglia disrupts neuronal-glial communication and promotes senescence. [Abstract]

2026 Jun 26. PMID: 42362883
Sci China Life Sci

Targeting methionine-induced pyroptosis enhances anti-tumor immunity. [Abstract]

2026 May;69(5):1604-1619. PMID: 41793590

FIDAS-5 purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2026 May;69(5):1604-1619. [Abstract]

iBMDM cells were cultured as indicated for cell viability, LDH release, and Western blot analyses. iBMDM cells were pre-cultured with Z-VAD-FMK before exposure to FIDAS-5 or PF-9366. Actin was the loading control.
Food Funct

Methionine restriction attenuates renal injury by suppressing macrophage polarization via IRF1 downregulation. [Abstract]

2025 Oct 27. PMID: 41140247
Development

S-adenosyl-L-methionine supplementation alleviates the destructed intestinal epithelium and inflammatory infiltration caused by Mat2a deficiency. [Abstract]

2023 Oct 15;150(20):dev201135. PMID: 36975381
bioRxiv

Radiation synergizes with BET inhibition to stimulate durable, systemic anti-tumor immunity in murine cancer models. [Abstract]

2026 Feb 18:2026.02.16.706212. PMID: 41757027
bioRxiv

2024 September 03.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 125 mg/mL (477.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.8209 mL

19.1044 mL

38.2088 mL

5 mM

0.7642 mL

3.8209 mL

7.6418 mL

10 mM

0.3821 mL

1.9104 mL

3.8209 mL

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (7.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (7.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Zhang W, et al. Fluorinated N,N-dialkylaminostilbenes repress colon cancer by targeting methionine S-adenosyltransferase 2A. ACS Chem Biol. 2013 Apr 19;8(4):796-803. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.8209 mL	19.1044 mL	38.2088 mL	95.5219 mL
	5 mM	0.7642 mL	3.8209 mL	7.6418 mL	19.1044 mL
	10 mM	0.3821 mL	1.9104 mL	3.8209 mL	9.5522 mL
	15 mM	0.2547 mL	1.2736 mL	2.5473 mL	6.3681 mL
	20 mM	0.1910 mL	0.9552 mL	1.9104 mL	4.7761 mL
	25 mM	0.1528 mL	0.7642 mL	1.5284 mL	3.8209 mL
	30 mM	0.1274 mL	0.6368 mL	1.2736 mL	3.1841 mL
	40 mM	0.0955 mL	0.4776 mL	0.9552 mL	2.3880 mL
	50 mM	0.0764 mL	0.3821 mL	0.7642 mL	1.9104 mL
	60 mM	0.0637 mL	0.3184 mL	0.6368 mL	1.5920 mL
	80 mM	0.0478 mL	0.2388 mL	0.4776 mL	1.1940 mL
	100 mM	0.0382 mL	0.1910 mL	0.3821 mL	0.9552 mL

Keywords

FIDAS-5

9 Cited Publications

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FIDAS-5

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) FIDAS-5

Biologische Aktivität

Chemical Information

Publications (9)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

FIDAS-5

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) FIDAS-5

Biologische Aktivität

Chemical Information

Publications (9)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

FIDAS-5 Related Classifications

Art. -Nr.: HY-136144