Art. -Nr.: HY-15121

L-Theanine

(Synonyms: L-Glutamic Acid γ-ethyl amide; Nγ-Ethyl-L-glutamine)

5 Cited Publications

Kundenbewertung

Based on 5 publication(s) in Google Scholar

L-Theanine (L-Glutamic Acid γ-ethyl amide) is a non-protein amino acid contained in green tea leaves, which blocks the binding of L-glutamic acid to glutamate receptors in the brain, and with neuroprotective, anticancer and anti-oxidative activities. L-Theanine can pass through the blood–brain barrier and is orally active.

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Reinheit: 99.64%
CAS. Nr.: 3081-61-6
Formel: C₇H₁₄N₂O₃
Molecular Weight:174.20

Speicherung:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

L-Theanine Chemical Structure

CAS. Nr.: 3081-61-6

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (5)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

In Vitro

L-Theanine (L-Glutamic Acid γ-ethyl amide) inhibits the incorporation of extracellular glutamine into neurons, resulting in the suppression of exocytotic release of glutamate^[3].
L-Theanine (500 μM; 72 h) protects against excess Dopamine (HY-B0451)-induced neuronal death in presence of astrocytes and increases glutathione level in astrocytes^[3].
L-Theanine (0-5 mM; 72 h) is involved in glutathione synthesis^[3].
L-Theanine (0.1-5 mM; 24 h) dose-dependently inhibits the viability of melanoma but not normal epidermal melanocytes^[4].
L-Theanine (1-5 mM; 24 h) arrests cell cycle at G0/G1 phase, suppresses cell migration, induces apoptosis in A375 cells^[4].
L-Theanine (1-5 mM; 24 h) also affects the proliferation, migration, and apoptosis of B16–F10 melanoma cells^[4].
L-Theanine shows protective effect on cadmium-induced apoptosis in PC12 cells by inhibiting the mitochondria-mediated pathway and decreased ROS production^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[4]

Cell Line:

A375 and PIG1 cells
Concentration:

0.1, 0.5, 1, 2 and 5 mM
Incubation Time:

24 h
Result:

Dose-dependently decreased the viability of A375, but not PIG1 cells.

Cell Cycle Analysis^[4]

Cell Line:

A375
Concentration:

1, 2 and 5 mM
Incubation Time:

24 h
Result:

Led to a dose dependent accumulation of A375 cells in G0/G1 phase and prevented cells from entering the S phase.

Western Blot Analysis^[4]

Cell Line:

A375
Concentration:

1, 2 and 5 mM
Incubation Time:

24 h
Result:

Remarkably reduced the expression of proliferating cell nuclear antigen (PCNA), decreased protein levels of cyclinD1, cyclinE1, and cyclin-dependent protein kinase (CDK2 and CDK4). Potentiated the expression of cyclin-dependent kinase inhibitor 1A (CDKN1A, p21).
Dose-dependently increased the levels of apoptosis-promoting proteins including BAX and cleaved-caspase3 and decreased the level of antiapoptotic protein BCL-2.
Concentration dependently reduced the protein levels of ICAM-1, VCAM-1, MMP9, and MMP2.
Dose-dependently increased the p53 expression.

Cell Migration Assay ^[4]

Cell Line:

A375
Concentration:

1, 2 and 5 mM
Incubation Time:

24 h
Result:

Apparently suppressed the migration.

Apoptosis Analysis^[4]

Cell Line:

A375
Concentration:

1, 2 and 5 mM
Incubation Time:

24 h
Result:

Showed pro-apoptotic effect.

RT-PCR^[4]

Cell Line:

A375
Concentration:

1, 2 and 5 mM
Incubation Time:

24 h
Result:

Markedly elevated the mRNA levels of Bmal1, Clock, Rora, and Rev-erbβ.

In Vivo

L-Theanine (4.0 mg/kg; p.o.; daily for 14 days) upregulates glutathione contents in striata of normal mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Healthy male ICR mice weighing 32–34 g^[3]
Dosage:

4.0 mg/kg
Administration:

Oral administration, daily for 14 days
Result:

Significantly increased glutathione content in the striatum, but not in the midbrain.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 3081-61-6
Appearance Solid
Molecular Weight 174.20
Formel C₇H₁₄N₂O₃
Color White to off-white
SMILES

OC([C@@H](N)CCC(NCC)=O)=O
Synonyms

L-Glutamic Acid γ-ethyl amide; Nγ-Ethyl-L-glutamine
Structure Classification
- Amino acids
Initial Source
- Microorganisms
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (5)

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Journal Impact Factor

Mol Med

L-theanine alleviates myocardial ischemia/reperfusion injury by suppressing oxidative stress and apoptosis through activation of the JAK2/STAT3 pathway in mice. [Abstract]

2024 Jun 28;30(1):98. PMID: 38943069
Phytother Res

l-Theanine Improves Learning and Memory Through Attenuation of NOX4-Mediated Ferroptosis in Hippocampal Neurons of Sleep-Deprived Mice. [Abstract]

2025 Jul 19. PMID: 40682446
Journal of Electroanalytical Chemistry 915 (2022) 116334
Poult Sci

L-Theanine attenuates oxidative damage induced by heat stress through the PI3K/AKT/Nrf2 signaling pathway in skeletal muscle cells. [Abstract]

2025 Apr 4;104(6):105140. PMID: 40203618
Naunyn Schmiedebergs Arch Pharmacol

L-theanine prevents myocardial injury in sleep‑deprived mice by suppressing ferroptosis through SIRT1. [Abstract]

2025 May 6. PMID: 40328913

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : 150 mg/mL (861.08 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.7405 mL

28.7026 mL

57.4053 mL

5 mM

1.1481 mL

5.7405 mL

11.4811 mL

10 mM

0.5741 mL

2.8703 mL

5.7405 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (574.05 mM); Clear solution; Need ultrasonic

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Vuong QV, et al. L-Theanine: properties, synthesis and isolation from tea. J Sci Food Agric. 2011 Aug 30;91(11):1931-9. [Content Brief]

[2]. Kimura K, et al. L-Theanine reduces psychological and physiological stress responses. Biol Psychol. 2007 Jan;74(1):39-45. [Content Brief]

[3]. Takeshima M, et al. l-Theanine protects against excess dopamine-induced neurotoxicity in the presence of astrocytes. J Clin Biochem Nutr. 2016 Sep;59(2):93-99. [Content Brief]

[4]. Zhang R, et al. L-Theanine inhibits melanoma cell growth and migration via regulating expression of the clock gene BMAL1. Eur J Nutr. 2022 Mar;61(2):763-777. [Content Brief]

[5]. Ben P, et al. Protective Effect of L-Theanine on Cadmium-Induced Apoptosis in PC12 Cells by Inhibiting the Mitochondria-Mediated Pathway. Neurochem Res. 2015 Aug;40(8):1661-70. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	5.7405 mL	28.7026 mL	57.4053 mL	143.5132 mL
	5 mM	1.1481 mL	5.7405 mL	11.4811 mL	28.7026 mL
	10 mM	0.5741 mL	2.8703 mL	5.7405 mL	14.3513 mL
	15 mM	0.3827 mL	1.9135 mL	3.8270 mL	9.5675 mL
	20 mM	0.2870 mL	1.4351 mL	2.8703 mL	7.1757 mL
	25 mM	0.2296 mL	1.1481 mL	2.2962 mL	5.7405 mL
	30 mM	0.1914 mL	0.9568 mL	1.9135 mL	4.7838 mL
	40 mM	0.1435 mL	0.7176 mL	1.4351 mL	3.5878 mL
	50 mM	0.1148 mL	0.5741 mL	1.1481 mL	2.8703 mL
	60 mM	0.0957 mL	0.4784 mL	0.9568 mL	2.3919 mL
	80 mM	0.0718 mL	0.3588 mL	0.7176 mL	1.7939 mL
	100 mM	0.0574 mL	0.2870 mL	0.5741 mL	1.4351 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

L-Theanine

5 Cited Publications

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L-Theanine

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Publications Citing Use of MedChemExpress (MCE) L-Theanine

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (5)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

L-Theanine Related Classifications

HY-15121 Related Classifications