Art. -Nr.: HY-N0683A

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rel-α-Vitamin E

(Synonyms: rel-D-α-Tocopherol)

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rel-α-Vitamin E (rel-D-α-Tocopherol) is a vitamin with antioxidant properties and also a mixture.

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Reinheit: 70.0%
CAS. Nr.: 2074-53-5
Formel: C₂₉H₅₀O₂
Molecular Weight:430.72

Speicherung:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

rel-α-Vitamin E Chemical Structure

CAS. Nr.: 2074-53-5

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

rel-α-Vitamin E (rel-D-α-Tocopherol) is a vitamin with antioxidant properties and also a mixture^[1].

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
Erythrocyte	IC₅₀	3.658 μg/mL Compound: alpha-tocopherol	Antioxidant activity in rat erythrocytes assessed as inhibition of phenyl hydrazine-induced haemolysis after 1 hr Antioxidant activity in rat erythrocytes assessed as inhibition of phenyl hydrazine-induced haemolysis after 1 hr	[PMID: 23395966]
Fibroblast	EC₅₀	>500 nM Compound: alpha-TOH	Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay	[PMID: 33214825]
Fibroblast	EC₅₀	234 nM Compound: 2, Vitamin E	Cytoprotective activity against BSO-induced human FRDA primary patient fibroblasts compound pretreated 12 hrs prior to BSO-induction measured after 48 hrs by calcein-AM-based cell viability assay Cytoprotective activity against BSO-induced human FRDA primary patient fibroblasts compound pretreated 12 hrs prior to BSO-induction measured after 48 hrs by calcein-AM-based cell viability assay	[PMID: 22137789]
Fibroblast	GI₅₀	>=300 μM Compound: 2, Vitamin E	Growth inhibition of human FRDA primary patient fibroblasts after 48 hrs by calcein-AM-based cell viability assay Growth inhibition of human FRDA primary patient fibroblasts after 48 hrs by calcein-AM-based cell viability assay	[PMID: 22137789]
HepG2	IC₅₀	>300 μM Compound: alpha-TOH	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]
HL-60	IC₅₀	255 μM Compound: Vitamin E	Antioxidant activity against TPA-induced ROS production in human HL60 cells by 2',7'-dichlorodihydrofluorescein diacetate cellular-based assay Antioxidant activity against TPA-induced ROS production in human HL60 cells by 2',7'-dichlorodihydrofluorescein diacetate cellular-based assay	[PMID: 12762791]
J774	IC₅₀	>500 μM Compound: 1	Cytotoxicity against mouse J774 cells by MTT assay Cytotoxicity against mouse J774 cells by MTT assay	[PMID: 17627828]
Lymphocyte	EC₅₀	>500 nM Compound: alpha-TOH	Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis	[PMID: 33214825]
MCF7	IC₅₀	>300 μM Compound: alpha-TOH	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]
MCF7	IC₅₀	329 μM Compound: 1 (alpha-TOH)	Compound was tested for antiproliferative activity against human breast cancer cell line MCF-7 in MTT assay Compound was tested for antiproliferative activity against human breast cancer cell line MCF-7 in MTT assay	[PMID: 9873556]
MT4	IC₅₀	165 μM Compound: alpha-TOH	Cytotoxicity against human MT4 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MT4 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]
RPMI-8226	IC₅₀	>300 μM Compound: alpha-TOH	Cytotoxicity against human RPMI8226 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human RPMI8226 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]
THP-1	IC₅₀	>500 μM Compound: 1	Cytotoxicity against human THP1 cells by MTT assay Cytotoxicity against human THP1 cells by MTT assay	[PMID: 17627828]
U-937	IC₅₀	100 μM Compound: alpha-TOH	Cytotoxicity against human U937 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human U937 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27344490]

In Vitro

Vitamin E is a lipid-soluble, chain-breaking type of antioxidant present in human blood. Vitamin E works as a free radical scavenger and has the primary function of destroying peroxyl radicals. Thus, it protects long-chain polyunsaturated fatty acids (e.g., cell membranes or low-density lipoprotein cholesterol) from oxidation or destruction. Its association with vitamin C is of great pathophysiological importance, because inhibition of lipid peroxidation by α-tocopherol occurs through its conversion into an oxidized α-tocopheroxyl radical, which in turn is regenerated to α-tocopherol through reduction by redox-active reagents^[1].
α-Tocopherol can inhibit the recruitment of lymphocytes and eosinophils^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In order to longitudinally relate vitamin E status, evaluated by means of α- and γ-tocopherol measured in erythrocytes membranes, to inflammatory state and oxidative stress by means of total antioxidant capacity of plasma and lipid peroxidation biomarkers, and to assess its association to hard long-term outcomes (e.g., overall and cause-specific graft and recipient loss), and to be able to likewise account for liver, lung, and heart transplant recipients^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 2074-53-5
Appearance Oil
Molecular Weight 430.72
Formel C₂₉H₅₀O₂
Color Light yellow to yellow
SMILES

OC1=C(C)C(C)=C2C(CC[C@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)O2)=C1C
Synonyms

rel-D-α-Tocopherol
Structure Classification
- Phenols
- Monophenols
- Others
Initial Source
- Plants
- other families
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

Ethanol : 50 mg/mL (116.08 mM; Need ultrasonic)

DMSO : 16.11 mg/mL (37.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3217 mL

11.6085 mL

23.2169 mL

5 mM

0.4643 mL

2.3217 mL

4.6434 mL

10 mM

0.2322 mL

1.1608 mL

2.3217 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.61 mg/mL (3.74 mM); Clear solution

This protocol yields a clear solution of ≥ 1.61 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.1 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.61 mg/mL (3.74 mM); Clear solution

This protocol yields a clear solution of ≥ 1.61 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.1 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Sotomayor CG, et al. Plasma versus Erythrocyte Vitamin E in Renal Transplant Recipients, and Duality of Tocopherol Species. Nutrients. 2019 Nov 19;11(11). pii: E2821. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / Ethanol	1 mM	2.3217 mL	11.6085 mL	23.2169 mL	58.0423 mL
	5 mM	0.4643 mL	2.3217 mL	4.6434 mL	11.6085 mL
	10 mM	0.2322 mL	1.1608 mL	2.3217 mL	5.8042 mL
	15 mM	0.1548 mL	0.7739 mL	1.5478 mL	3.8695 mL
	20 mM	0.1161 mL	0.5804 mL	1.1608 mL	2.9021 mL
	25 mM	0.0929 mL	0.4643 mL	0.9287 mL	2.3217 mL
	30 mM	0.0774 mL	0.3869 mL	0.7739 mL	1.9347 mL
Ethanol	40 mM	0.0580 mL	0.2902 mL	0.5804 mL	1.4511 mL
	50 mM	0.0464 mL	0.2322 mL	0.4643 mL	1.1608 mL
	60 mM	0.0387 mL	0.1935 mL	0.3869 mL	0.9674 mL
	80 mM	0.0290 mL	0.1451 mL	0.2902 mL	0.7255 mL
	100 mM	0.0232 mL	0.1161 mL	0.2322 mL	0.5804 mL

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rel-α-Vitamin E

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Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

rel-α-Vitamin E

Antioxidant Compound Library

Oxygen Sensing Compound Library

Antibacterial Compound Library

Ferroptosis Compound Library

Phenols Library

Plant-Sourced Natural Product Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Bioactive Compound Library

Anti-Fibrosis Compound Library

Vitamin Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Lactylation Compound Library

Natural Product and Natural Product-Like Compound Library

Anti-Infection Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Natural Product Library

Antiviral Compound Library

Anti-Aging Compound Library

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

rel-α-Vitamin E Related Classifications

HY-N0683A Related Classifications