Art. -Nr.: HY-139665

VB124

8 Cited Publications

Kundenbewertung

Based on 8 publication(s) in Google Scholar

VB124 is an orally active, potent, and selective MCT4 inhibitor. VB124 can specifically inhibit lactate efflux with IC₅₀s of 8.6 nM and 19 nM for lactate import and export in MDA-MB-231 cells, respectively. VB124 is highly selective for MCT4 over MCT1. VB124 can be used for the research of cardiac hypertrophy, heart failure, and metabolism.

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Reinheit: 98.91%
CAS. Nr.: 2230186-18-0
Formel: C₂₃H₂₃ClN₂O₄
Molecular Weight:426.89

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

VB124 Chemical Structure

CAS. Nr.: 2230186-18-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (8)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

MCT4

Alle Monocarboxylate Transporter Isoform-spezifische Produkte anzeigen :

In Vitro

VB124 (10 μM) inhibits the cell proliferation of MDA-MB-231 cells, and the cell proliferation rate is less than 50%^[1].
VB124 is highly selective for MCT4 over MCT1, showing very little MCT1 inhibitory activity (lactate export IC₅₀=24 μM) in MCT1-expressing BT20 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

VB124 (30 mg/kg; p.o.; daily for 28 days) attenuates isoproterenol-induced cardiac hypertrophy in mice^[1].
VB124 (30 mg/kg; twice per day for 180 days) has no effect on the body, heart, liver, or lung weight of mice, suggesting no overt toxicities^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

12 weeks old C57BL/6 mice^[1]
Dosage:

30 mg/kg
Administration:

Oral gavage; daily for 28 days (dissolved in 0.5% methylcellulose and 0.1% Tween-20)
Result:

Prevented cardiac hypertrophy in mice.

Chemical Information

CAS. Nr. 2230186-18-0
Appearance Solid
Molecular Weight 426.89
Formel C₂₃H₂₃ClN₂O₄
Color White to off-white
SMILES

CC(C)(OCC1=NN(C2=CC=CC=C2Cl)C(C3=CC=CC(OC4CC4)=C3)=C1)C(O)=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (8)

All Cell Proliferation/Viability AssayWBFlow CytometryIn Vivo Efficacy Study

Sort by:

Journal Impact Factor

Cell Metab

Lactate controls cancer stemness and plasticity through epigenetic regulation. [Abstract]

2025 Apr 1;37(4):903-919.e10. PMID: 39933514
Nat Metab

Intracellular metabolic gradients dictate dependence on exogenous pyruvate. [Abstract]

2025 Jun;7(6):1168-1182. PMID: 40295877
Cell Rep Med

ACVR2A attenuation impacts lactate production and hyperglycolytic conditions attracting regulatory T cells in hepatocellular carcinoma. [Abstract]

2025 Mar 18:102038. PMID: 40139191

VB124 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Mar 18:102038. [Abstract]

Dose-response curves of the MCT4 inhibitor VB124 in HuH7 KO and Hepa1-6 KO cells.

VB124 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Mar 18:102038. [Abstract]

Colony formation assays of HuH7 KO and Hepa1-6 KO cells treated with VB124 (10 μM).

VB124 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Mar 18:102038. [Abstract]

KD of LDHA and treatment with the MCT1 inhibitor AR-C155858 and the MCT4 inhibitor VB124 (1, 10, 20 nM) significantly reduced the population of Foxp-3⁺ Treg cells.

VB124 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Mar 18:102038. [Abstract]

Tumorigenicity assay of Hepa1-6 KO cells in immunoproficient mice treated with anti-PD-1 antibody and VB124 (30 mg/kg, p.o.).
Mol Biomed

Lactate-driven pyrimidine synthesis promotes ferroptosis resistance in hepatocellular carcinoma. [Abstract]

2026 Apr 17;7(1):53. PMID: 41996004
Cell Rep

Macrophage MCT4 inhibition activates reparative genes and protects from atherosclerosis by histone H3 lysine 18 lactylation. [Abstract]

2024 May 9;43(5):114180. PMID: 38733581
Mol Metab

microRNA-1 regulates metabolic flexibility by programming adult skeletal muscle pyruvate metabolism. [Abstract]

2025 Aug:98:102182. PMID: 40490136
Chin Med

HuoXue QianYang QuTan recipe attenuates myocardial hypertrophy in obese hypertensive rats by regulating MPC1/MCT4 mediated pyruvate-lactate metabolic axis. [Abstract]

2025 Oct 23;20(1):180. PMID: 41131633

VB124 purchased from MedChemExpress. Usage Cited in: Chin Med. 2025 Oct 23;20(1):180. [Abstract]

Representative immunoblot demonstrating β-MHC and ANP protein expression in H9C2 cells treated with VB124
Res Sq

2025 May 08.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (234.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3425 mL

11.7126 mL

23.4252 mL

5 mM

0.4685 mL

2.3425 mL

4.6850 mL

10 mM

0.2343 mL

1.1713 mL

2.3425 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (5.86 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Cluntun AA, et.al. The pyruvate-lactate axis modulates cardiac hypertrophy and heart failure. Cell Metab. 2021 Mar 2;33(3):629-648.e10. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3425 mL	11.7126 mL	23.4252 mL	58.5631 mL
	5 mM	0.4685 mL	2.3425 mL	4.6850 mL	11.7126 mL
	10 mM	0.2343 mL	1.1713 mL	2.3425 mL	5.8563 mL
	15 mM	0.1562 mL	0.7808 mL	1.5617 mL	3.9042 mL
	20 mM	0.1171 mL	0.5856 mL	1.1713 mL	2.9282 mL
	25 mM	0.0937 mL	0.4685 mL	0.9370 mL	2.3425 mL
	30 mM	0.0781 mL	0.3904 mL	0.7808 mL	1.9521 mL
	40 mM	0.0586 mL	0.2928 mL	0.5856 mL	1.4641 mL
	50 mM	0.0469 mL	0.2343 mL	0.4685 mL	1.1713 mL
	60 mM	0.0390 mL	0.1952 mL	0.3904 mL	0.9761 mL
	80 mM	0.0293 mL	0.1464 mL	0.2928 mL	0.7320 mL
	100 mM	0.0234 mL	0.1171 mL	0.2343 mL	0.5856 mL

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VB124

Membrane Transporter/Ion Channel Compound Library

Anti-Cardiovascular Disease Compound Library

Orally Active Compound Library

Anti-Cancer Metabolism Compound Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Lactic Acid Metabolic Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) VB124

Alle Monocarboxylate Transporter Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (8)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

VB124

Membrane Transporter/Ion Channel Compound Library

Anti-Cardiovascular Disease Compound Library

Orally Active Compound Library

Anti-Cancer Metabolism Compound Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Lactic Acid Metabolic Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) VB124

Alle Monocarboxylate Transporter Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (8)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

VB124 Related Classifications

HY-139665 Related Classifications