D-Luciferin

D-Luciferin purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Mar 28;11(13):eads2295. [Abstract]

Customer Validation
Briefly, d-luciferin sodium (150 mg/kg in PBS) was injected intraperitoneally into the mice. Fifteen minutes later, mice were euthanized, and the heart, liver, spleen, lungs, kidneys, and spinal cord were collected. Bioluminescence images (yellow-green light) in these tissues were analyzed using Living Image Software (PerkinElmer). Representative in vivo imaging system images of major organs in SCI mice after being administered with firefly luciferase (FLuc) mRNA-LNPs (0.5 mg mRNA/kg).

D-Luciferin purchased from MedChemExpress. Usage Cited in: J Med Chem. 2024 Nov 28;67(22):20100-20117. [Abstract]

Customer Validation
The tumor model was established by tail vein injection of luciferase-labeled MOLM-13-Luc cells. After allowing 6 days for tumor growth post-inoculation, daily oral gavage treatment was initiated (designated as day 0 of treatment) at doses of 200 or 400 mg/kg. Subsequently, bioluminescence imaging was performed via intraperitoneal injection of 150 mg/kg D-Luciferin potassium.

D-Luciferin purchased from MedChemExpress. Usage Cited in: mBio. 2025 Apr 9;16(4):e0366524. [Abstract]

Customer Validation
Luciferase complementation imaging assay (LCA) of the interactions between BPA1 and either RipD or ACD11 in N. benthamiana. The interaction of Ca1 (AT3G01500) and Ca4 (AT1G70410) was used as a positive control, and n-Luc or c-Luc was used as a negative control.
The interacting target proteins are fused to either the N-terminal or C-terminal fragment of luciferase, and the recombinant constructs are transformed into Agrobacterium tumefaciens GV3101. Forty hours after agroinfiltration,

D-Luciferin purchased from MedChemExpress. Usage Cited in: ACS Nano. 2024 Dec 10;18(49):33366-33380. [Abstract]

Customer Validation
4T1-luc tumor-bearing BALB/c mice were intravenously (i.v.) injected with 100 mg/kg of iLNPs and 100 mg/kg piLNPs. D-Luciferin sodium (150 mg/kg) was administered to mice 5 min before fluorescence measurement, and ICG fluorescence was measured using an in vivo imaging system (IVIS) at each indicated time.

D-Luciferin purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Aug 16;10(33):eado1533. [Abstract]

Customer Validation
Monitoring of primary tumor growth and the occurrence of lung metastasis was conducted using bioluminescence imaging after intraperitoneal injection of D-luciferin sodium (150 mg/kg).

D-Luciferin purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2023 Sep 15.

Customer Validation
In vivo bioluminescence images of mice in different groups. Lung metastasis of the tumors through in vivo imaging by intraperitoneal injection of D-luciferin sodium (75 mg/kg, 100 μL) at 20 min before bioluminescence imaging.

D-Luciferin purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2023 Dec;13(12):5074-5090. [Abstract]

Customer Validation
The bioluminescence images of lungs harvested from mice post different treatments. On day 13 after intravenous injection of B16F10-luc cells to establish a lung metastasis model of melanoma in mice, D-luciferin potassium (150 mg/kg) is intraperitoneally injected into the mice.

D-Luciferin purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Oct 19;21(1):383. [Abstract]

Customer Validation
On day 15 after intravenous injection of B16F10-luc cells into C57BL/6 mice to establish a melanoma lung metastasis model, D-Luciferin potassium (150 mg/kg) is intrabitoneally injected into the mice, and lung tissue is collected from the mice for bioluminescence imaging.

D-Luciferin purchased from MedChemExpress. Usage Cited in: New Phytol. 2023 Sep;239(5):1887-1902. [Abstract]

Customer Validation
The full-length coding sequences (CDS) of target genes are cloned into either the pCAMBIA-nLuc or pCAMBIA-cLuc vectors. The resulting recombinant constructs are transformed into Agrobacterium tumefaciens strain GV3101, which is then infiltrated into Nicotiana benthamiana leaves. After 2 days, the infiltrated leaves are painted with 1 mM D-Luciferin potassium.

D-Luciferin purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2023 Mar;13(3):967-981. [Abstract]

Customer Validation
Tumor growth is monitored by bioluminescence imaging. Prior to imaging, D-luciferin substrate is injected intraperitoneally into the mice (150 mg/kg per mouse).

D-Luciferin purchased from MedChemExpress. Usage Cited in: New Phytol. 2022 Dec;236(5):1796-1808. [Abstract]

Customer Validation
ERF16-nLUC and CaM2-cLUC vectors are cotransfected in N. benthamiana leaves. 10 min after the abaxial sides of leaves are sprayed with 1 mM Luciferin.

D-Luciferin purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2021 Apr;166:105527. [Abstract]

Customer Validation
After cell inoculation for 7 days, mice were injected intraperitoneally with D-luciferin substrate at 150 mg/kg and anesthetized with 10% chloral hydrate (5 mL/kg).

D-Luciferin purchased from MedChemExpress. Usage Cited in: Cell Mol Gastroenterol Hepatol. 2021;12(3):839-856. [Abstract]

Customer Validation
HEK293T cells were seeded into a 6-well plate and transfected with in vitro synthesized mRNA containing the luciferase gene by Lipofectamine 2000. Luciferase substrate D-Luciferin sodium salt (1 mM) was added into the culture medium immediately after transfection. Luciferase activity was detected on the GloMax 96 Microplate Luminometer.

D-Luciferin purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Sep 17;11(9):765. [Abstract]

Customer Validation
At Day 10, 25, and 31, mice were anesthetized with isoflurane and then injected with 75 mg/kg D-Luciferin solution for imaging.