3′-Oxolutein
3′-Oxolutein, a metabolite of dietary lutein, is an anti-inflammatory agent and antioxidant, 3′-Oxolutein mitigates the effects of glutamate-induced ROS production and proinflammatory cytokine secretions in SH-SY5Y cells. 3'-Oxolutein reduces glutamate-induced iron content and increases thiol levels. 3′-Oxolutein can be used for the study of inflammation.
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- CAS No.: 23984-55-6
- Formule: C40H54O2
- Masse moléculaire:566.86
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
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Activité biologique
Description
In Vitro
3'-Oxolutein (10 ng/μL, 10-30 min) significantly reduces glutamate-induced ROS production in SH-SY5Y cells[1].
3'-Oxolutein (10 ng/μL, 24-72 h) increases small molecule antioxidants level at 48 h but fails to modify TAC levels in glutamate-treated SH-SY5Y cells[1].
3'-Oxolutein (10 ng/μL, 24-72 h) alone does not alter SOD activity and cannot inhibit the decrease in SOD in glutamate-treated SH-SY5Y cells[1].
3'-Oxolutein (10 ng/μL, 24-72 h) reduces glutamate-induced iron content in SH-SY5Y cells[1].
3'-Oxolutein (10 ng/μL, 24-72 h) reduces glutamate-induced relative mRNA expressions of ALOX5, ALOX15, and ACSL4 genes in SH-SY5Y cells[1].
3'-Oxolutein (10 ng/μL, 24-72 h) does not induce alteration in IL-6 secretion in SH-SY5Y cells but reduces glutamate-induced IL-8 secretions and diminishes TNFα levels[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:SH-SY5Y cells
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Concentration:10 ng/μL
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Incubation Time:24, 48, 72 h
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Result:Reduced relative mRNA expressions of ALOX5, ALOX15, and ACSL4 genes in OG1 and OG5 treatments compared to glutamate-treated SH-SY5Y cells.
Chemical Information
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CAS No. 23984-55-6
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Masse moléculaire 566.86
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Formule C40H54O2
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SMILES
CC1(C)C(/C=C/C(C)=C/C=C/C(C)=C/C=C/C=C(C)/C=C/C=C(C)/C=C/[C@@H]2C(C)(CC(C=C2C)=O)C)=C(C)C[C@@H](O)C1
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Synonyms
3'-Dehydrolutein; Philosamiaxanthin
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
3′-Oxolutein Related Classifications
HY-W743769 Related Classifications
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