Cat. No.: HY-B1065

Aceglutamide

(Synonyms: α-N-Acetyl-L-glutamine; N2-Acetylglutamine)

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Aceglutamide (α-N-Acetyl-L-glutamine; N2-Acetylglutamine) is a neuroprotectant that can penetrate the blood-brain barrier. Aceglutamide can enhance the antioxidant systems of glutathione (GSH), thioredoxin (Trx) and Nrf2. Aceglutamide also inhibits ASK1 and TRAF1, activates the Akt/Bcl-2 anti-apoptotic pathway, enhances the activity of antioxidant enzymes and reduces oxidative damage. Aceglutamide can improve neurological deficits after cerebral ischemia, reduce infarct volume, and inhibit neuronal apoptosis, especially substantia nigra dopaminergic neurons. Aceglutamide can reduce cerebral ischemia/reperfusion injury, improve motor dysfunction, and is used in ischemic stroke-related research.

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Pureté: 98.0%
CAS No.: 2490-97-3
Formule: C₇H₁₂N₂O₄
Masse moléculaire:188.18

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Aceglutamide Chemical Structure

CAS No.: 2490-97-3

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Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

Human Endogenous Metabolite

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In Vitro

Aceglutamide (1-10 μM; 24 h) improves the cell viability of H₂O₂-induced PC12 cells, reduces the levels of reactive oxygen species (ROS) and nitric oxide (NO), upregulates glutathione (GSH) content and activates the Nrf2/Trx antioxidant system^[1].
Aceglutamide (10 μM; 24 h) inhibits apoptosis in hypoxia/reoxygenation-injured PC12 cells and primary midbrain neurons, improves mitochondrial membrane potential (MMP), downregulates the pro-apoptotic factor TRAF1 and upregulates the Akt/Bcl-2/Bax signaling pathway^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

PC12 cells (rat pheochromocytoma cell line)
Concentration:

0.01 μM, 0.1 μM, 1 μM, 10 μM
Incubation Time:

24 h (post hypoxia/reoxygenation)
Result:

CCK8 assay showed 1 μM and 10 μM Aceglutamide significantly increased cell viability by 18% and 25% compared to the hypoxia/reoxygenation control group, respectively.

Apoptosis Analysis^[2]

Cell Line:

PC12 cells (rat pheochromocytoma cell line)
Concentration:

0.01 μM, 0.1 μM, 1 μM, 10 μM
Incubation Time:

24 h (post hypoxia/reoxygenation)
Result:

Flow cytometry (Annexin V/PI staining) revealed that 10 μM Aceglutamide reduced early/late apoptotic cells from 39.8% to 21.9%, accompanied by a dose-dependent decrease in apoptotic marker expression.

In Vivo

Aceglutamide (50, 150, 300 mg/kg; intraperitoneal injection; once daily; for 14 consecutive days) improves neurological deficits and reduces cerebral infarct volume in a dose-dependent manner in the cerebral ischemia/reperfusion (MCAO) model in male Sprague-Dawley rats, and exerted protective effects by inhibiting the apoptosis of substantia nigra dopaminergic neurons, upregulating the Akt/Bcl-2 pathway, and downregulating TRAF1 protein expression^[1]^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Sprague-Dawley rats (250-270 g, adult), transient middle cerebral artery occlusion (MCAO) model^[1]^[2]
Dosage:

50 mg/kg, 150 mg/kg, 300 mg/kg
Administration:

Intraperitoneal injection (i.p.), once daily, starting 24 h after reperfusion, for 14 consecutive days
Result:

Behavioral Tests: At 14 days post-reperfusion, 150 mg/kg and 300 mg/kg Aceglutamide significantly reduced neurological severity scores (NSS) and adhesive-removal time, and improved beam-walking performance, indicating enhanced motor and somatosensory function compared to the vehicle group^[1][2]. Infarct Volume: TTC staining showed 150 mg/kg and 300 mg/kg Aceglutamide reduced infarct volume ratio by 40% and 54%, respectively, compared to the vehicle group (32.13% vs. 19.22% and 14.76%)^[1]. Neuronal Protection: Immunohistochemistry revealed a significant increase in TH-positive neurons in the substantia nigra (SN) and TH-positive fiber density in the striatum in 150 mg/kg and 300 mg/kg groups, with 300 mg/kg increasing SN TH-positive cells by 35% compared to the vehicle group^[2].

Chemical Information

CAS No. 2490-97-3
Appearance Solid
Masse moléculaire 188.18
Formule C₇H₁₂N₂O₄
Color White to off-white
SMILES

O=C(N)CC[C@@H](C(O)=O)NC(C)=O
Synonyms

α-N-Acetyl-L-glutamine; N2-Acetylglutamine
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Microorganisms
- Endogenous metabolite
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvant et solubilité

In Vitro:

H₂O : 33.33 mg/mL (177.12 mM; Need ultrasonic)

DMSO : 16.67 mg/mL (88.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.3141 mL

26.5703 mL

53.1406 mL

5 mM

1.0628 mL

5.3141 mL

10.6281 mL

10 mM

0.5314 mL

2.6570 mL

5.3141 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.67 mg/mL (8.87 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 1.67 mg/mL (8.87 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.67 mg/mL (8.87 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 20 mg/mL (106.28 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (278 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Zhang J, et al. Enhanced thioredoxin, glutathione and Nrf2 antioxidant systems by safflower extract and aceglutamide attenuate cerebral ischaemia/reperfusion injury. J Cell Mol Med. 2020 May;24(9):4967-4980. [Content Brief]

[2]. Zhang R, et al. Neuroprotective effects of Aceglutamide on motor function in a rat model of cerebral ischemia and reperfusion. Restor Neurol Neurosci. 2015;33(5):741-59. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	5.3141 mL	26.5703 mL	53.1406 mL	132.8515 mL
	5 mM	1.0628 mL	5.3141 mL	10.6281 mL	26.5703 mL
	10 mM	0.5314 mL	2.6570 mL	5.3141 mL	13.2852 mL
	15 mM	0.3543 mL	1.7714 mL	3.5427 mL	8.8568 mL
	20 mM	0.2657 mL	1.3285 mL	2.6570 mL	6.6426 mL
	25 mM	0.2126 mL	1.0628 mL	2.1256 mL	5.3141 mL
	30 mM	0.1771 mL	0.8857 mL	1.7714 mL	4.4284 mL
	40 mM	0.1329 mL	0.6643 mL	1.3285 mL	3.3213 mL
	50 mM	0.1063 mL	0.5314 mL	1.0628 mL	2.6570 mL
	60 mM	0.0886 mL	0.4428 mL	0.8857 mL	2.2142 mL
	80 mM	0.0664 mL	0.3321 mL	0.6643 mL	1.6606 mL
H₂O	100 mM	0.0531 mL	0.2657 mL	0.5314 mL	1.3285 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Aceglutamide

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Voir tous les produits spécifiques à Isoform Akt

Activité biologique

Chemical Information

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Aceglutamide Related Classifications

HY-B1065 Related Classifications