Cardionogen-2
Cardionogen-2 is a biphasic modulator of cardiogenesis, either promoting or inhibiting heart formation depending on the stage of treatment. Cardionogen-2 inhibits Wnt/β-catenin-dependent transcription in murine embryonic stem (ES) cells and zebrafish embryos.
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- CAS No.: 578755-52-9
- Formule: C16H13N5O2S
- Masse moléculaire:339.37
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
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- Pureté et documentation
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Activité biologique
Description
In Vitro
In zebrafish embryos and murine embryonic stem (ES) cells, Cardionogen-2 treatment promotes cardiogenesis during and after gastrulation, whereas it inhibits heart formation before gastrulation. Cardionogen-2-induced effects can be antagonized by increasing Wnt/β-catenin signaling activity. Cardionogen-2 inhibits Wnt/β-catenin-dependent transcription in murine ES cells and zebrafish embryos[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 578755-52-9
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Masse moléculaire 339.37
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Formule C16H13N5O2S
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SMILES
COC(C=CC(C1=NN2C(C3=CC=CC=N3)=NN=C2S1)=C4)=C4OC
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Human pluripotent stem cell cardiomyocyte differentiation
Human pluripotent stem cell cardiomyocyte differentiation is commonly driven by timed modulation of developmental signaling: early Wnt/β-catenin activation through GSK3 inhibition promotes mesoderm induction, and subsequent Wnt inhibition promotes cardiac specification; this principle was demonstrated in defined, growth-factor-free monolayer systems that generated functional cardiomyocytes from multiple hPSC lines. The experimental readout is the emergence of cardiomyocyte identity and function, measured by spontaneous contraction, immunostaining or flow cytometry for cardiac proteins such as cTnT, α-actinin, MLC2a, or sarcomeric myosin, and functional assays such as electrophysiology or calcium/action-potential responses when required.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cardionogen-2 Related Classifications
HY-115474 Related Classifications
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