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FM4-64

22 Cited Publications
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Based on 22 publication(s) in Google Scholar

FM4-64 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM4-64 is widely used in endocytic and exospic membrane structure markers.

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  • Pureté: 99.94%
  • CAS No.: 162112-35-8
  • Formule: C30H45Br2N3
  • Masse moléculaire:607.51
  • Stockage:

    -20°C, sealed storage, away from moisture and light

    * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

  • FM4-64 Chemical Structure
  • Experimental Validation Results for FM4-64
  • Experimental Validation Results for FM4-64
  • Experimental Validation Results for FM4-64
  • Experimental Validation Results for FM4-64
  • Experimental Validation Results for FM4-64
  • FM4-64 Chemical Structure
    FM4-64 Chemical Structure
  • Experimental Validation Results for FM4-64
  • Experimental Validation Results for FM4-64
  • Experimental Validation Results for FM4-64
  • Experimental Validation Results for FM4-64
  • Experimental Validation Results for FM4-64
CAS No.: 162112-35-8
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Publications Citing Use of MedChemExpress (MCE) FM4-64

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Cell Imaging/Staining
  • Cell Imaging/Staining
  • Cell Imaging/Staining
  • Cell Imaging/Staining
  • Cell Imaging/Staining
  • Cell Imaging/Staining
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  • FM4-64 Chemical Structure
    FM4-64 Chemical Structure
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  • 1/16

    FM4-64 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Jul;14(7):e70131. [Abstract]

    Customer Validation
     FM4-64 (1 µM; 30 min). Confocal microscopy images of MG1655 stained with SYTOX (nucleic acid dye, green) and FM4‐64 (outer membrane dye, red) revealed that the SYTOX/FM4‐64 ratio increased under ENR treatment, indicating elevated membrane permeability.

  • 1/16

    FM4-64 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Jul;14(7):e70131. [Abstract]

    Customer Validation
     FM4-64 (1 µM; 30 min). Fluorescence microscopy indicated no significant change in peptidoglycan integrity in ENR-treated ΔrecA strains compared to untreated controls.

  • 1/16

    FM4-64 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Jul;14(7):e70131. [Abstract]

    Customer Validation
     FM4-64 (1 µM; 30 min). Fluorescence microscopy analysis using the SYTOX and FM4‐64 revealed increased cytoplasmic staining in both MG1655 and endolysin‐deficient strains (Δessd-1ΔrrrdΔrzod) under ENR stress, indicating membrane damage.

  • 1/16

    FM4-64 purchased from MedChemExpress. Usage Cited in: Microbiome. 2024 Dec 21;12(1):272. [Abstract]

    Customer Validation
     FM4-64 (20 μg/mL; 1 min). Representative images obtained from confocal laser scanning microscopy to analyze the behavior of AMP1. B. subtilis cells were treated with coumarin 343-labeled AMP1 at a concentration of 100 μM for various durations before observation. During observation, the B. subtilis cells membrane was stained with FM4-64.

  • 1/16

    FM4-64 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 12:e2406473. [Abstract]

    Customer Validation
     FM4-64 (1 µg/mL; 30 min). Confocal micrographs of vacuole staining (FM4‐64) and localization of Atg8 proteins in the GFP‐Atg8 mutant cells following the indicated treatment. DIC is Differential Interference Contrast; FM4‐64 was visualized as red fluorescent; Atg 8 proteins were visualized as green fluorescent.

  • FM4-64