Trypsin (MS grade)
Based on 1 Customer Validation
Trypsin MS grade is a serine protease enzyme, and hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin MS grade activates PAR2 and PAR4. Trypsin MS grade induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin MS grade also promotes cell proliferation and differentiation. Trypsin MS grade can be used in the research of wound healing and neurogenic inflammation.
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- CAS No.: 9002-07-7
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
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PAR2 |
PAR4 |
Trypsin MS grade (5 μg/mL, 24 or 48 h) promotes porcine deltacoronavirus (PDCoV) replication in LLC-PK cells[2].
Trypsin MS grade (10 and 50 ng/mL, 12 h) enhances PDCoV cell-to-cell spread in LLC-PK cells by promoting membrane fusion in LLC-PK cells[2].
Trypsin MS grade (0.05%, 3 h) promotes C6 glioma cell proliferation in serum-free and growth factor-free medium[3].
Trypsin MS grade (20 -150 ng/mL, 5 days) potentiates PBMC differentiation[4].
This product can be used for protein sequence analysis, such as protein mass spectrometry, sequencing, and peptide map analysis.
Protocol
This product is recommended to be dissolved or diluted with 1 mM HCl. The recommended enzymatic digestion ratio is recombinant trypsin: target protein = 1:20-1:100, and the optimal pH is 7.0-8.0.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.4.21.4
≥6200 USP U/mg soild
One USP unit is defined as the amount of enzyme that at 25°C and pH 7.6, in reaction system 3.2 mL (1 cm light path), hydrolysis of BAEE per minute increased the absorption value at 253 nm by 0.003.
Chemical Information
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CAS No. 9002-07-7
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Appearance Solid
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Color White to off-white
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SMILES
[Trypsin (MS grade)]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
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Fiche technique (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
[2]. Yue-Lin Yang,et al. Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner. Emerg Microbes Infect. 2020 Feb 24;9(1):457-468. [Content Brief]
[3]. H Amano, et al. Trypsin promotes C6 glioma cell proliferation in serum- and growth factor-free medium. Neurosci Res. 1996 Jul;25(3):203-8. [Content Brief]
[5]. R Costa, et al. Evidence for the role of neurogenic inflammation components in trypsin-elicited scratching behaviour in mice. Br J Pharmacol. 2008 Jul;154(5):1094-103. [Content Brief]
[6]. F Schmidlin, et al. Protease-activated receptors: how proteases signal to cells. Curr Opin Pharmacol. 2001 Dec;1(6):575-82. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Trypsin (MS grade) Related Classifications
HY-129047A Related Classifications
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