2. Apoptosis
  3. Apoptosis
  4. 5β,6β-Epoxycholestanol

5β,6β-Epoxycholestanol  (Synonyms: Cholesterol 5β,6β-epoxide; 5β,6β-Epoxycholesterol)

Cat. No.: HY-130502 Purity: 98.0%
COA
SDS
Handling Instructions Technical Support

5β,6β-epoxycholestanol (Cholesterol 5β,6β-epoxide; 5β,6β-Epoxycholesterol) is an oxysterol. 5β,6β-epoxycholestanol induces cytotoxicity in bronchial epithelial cells. 5β,6β-epoxycholestanol induces lactate dehydrogenase (LDH) release and apoptosis in lymphoma cells undergoing macrophage differentiation. 5β,6β-epoxycholestanol is applicable to research related to atherosclerosis.

For research use only. We do not sell to patients.

5β,6β-Epoxycholestanol

5β,6β-Epoxycholestanol Chemical Structure

CAS No. : 4025-59-6

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Based on 1 publication(s) in Google Scholar

Other Forms of 5β,6β-Epoxycholestanol:

5β,6β-Epoxycholestanol Related Antibodies

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  • Purity & Documentation

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Description

5β,6β-epoxycholestanol (Cholesterol 5β,6β-epoxide; 5β,6β-Epoxycholesterol) is an oxysterol. 5β,6β-epoxycholestanol induces cytotoxicity in bronchial epithelial cells. 5β,6β-epoxycholestanol induces lactate dehydrogenase (LDH) release and apoptosis in lymphoma cells undergoing macrophage differentiation. 5β,6β-epoxycholestanol is applicable to research related to atherosclerosis[1][2][3].

Cellular Effect
Cell Line Type Value Description References
HT-29 IC50
27.5 μM
Compound: 3
Cytotoxicity against human HT-29 cells after 96 hrs by Alamar blue assay
Cytotoxicity against human HT-29 cells after 96 hrs by Alamar blue assay
[PMID: 20931970]
HT-29 IC50
37.6 μM
Compound: 1b
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay
[PMID: 19473028]
HT-29 IC50
37.6 μM
Compound: 3
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
In Vitro

5β,6β-epoxycholestanol (10-30 μM; 50 h) is dose- and time-dependently cytotoxic to 16-HBE cells, with 30 μM inducing significant death by 24 h and 10 μM inducing over 50% death by 48 h[1].
5β,6β-epoxycholestanol (0.03-10 μM; 24 h pre-incubation; 3 h [14C]acetate incubation) potently inhibits cholesterol biosynthesis in 16-HBE cells with an IC50 of 350 nM and in A549 cells with an IC50 of 150 nM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5β,6β-Epoxycholestanol Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: 16-HBE cells
Concentration: 10 μM; 20 μM; 30 μM
Incubation Time: 50 h
Result: Induced dose- and time-dependent cytotoxicity.
Caused significant cell death in 24 h at 30 μM dose.
Induced over 50% cell death in 48 h at 10 μM dose.
Showed no cell death with 30 μM α-epoxide after 40 h.
Molecular Weight

402.65

Formula

C27H46O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@]12[C@@]3(C[C@H](CC2)O)[C@](C[C@]4([H])[C@]1([H])CC[C@]5([C@@]4([H])CC[C@]5([H])[C@H](C)CCCC(C)C)C)([H])O3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : 20 mg/mL (49.67 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4835 mL 12.4177 mL 24.8355 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

SDS (418 KB)

COA (248 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 2.4835 mL 12.4177 mL 24.8355 mL 62.0887 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL 12.4177 mL
10 mM 0.2484 mL 1.2418 mL 2.4835 mL 6.2089 mL
15 mM 0.1656 mL 0.8278 mL 1.6557 mL 4.1392 mL
20 mM 0.1242 mL 0.6209 mL 1.2418 mL 3.1044 mL
25 mM 0.0993 mL 0.4967 mL 0.9934 mL 2.4835 mL
30 mM 0.0828 mL 0.4139 mL 0.8278 mL 2.0696 mL
40 mM 0.0621 mL 0.3104 mL 0.6209 mL 1.5522 mL
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5β,6β-Epoxycholestanol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5β,6β-Epoxycholestanol4025-59-6Cholesterol 5β,6β-epoxide 5β,6β-EpoxycholesterolApoptosispulmonary surfactantatherosclerosismicrosomal cholesterol 7α-hydroxylaseA549 cellsmicrosomal hydrolasecholestan-3β5α6β-triolisoprenoid-based cholesterol biosynthesis pathwaycholestan-6-oxo-3β5α-diolhuman bronchial epithelial cellsoxysterolInhibitorinhibitorinhibit

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