Clomiphene citrate
Based on 5 publication(s) in Google Scholar
Clomiphene citrate (Clomifene citrate) is an orally active estrogen-receptor modulator. Clomiphene citrate has anti-cancer actixity, induces perturbations during meiotic maturation and cytogenetic abnormalities and ameliorates in managing psychiatric and cognitive impairment.
For research use only. We do not sell to patients.
- Purity: 98.22%
- CAS No.: 50-41-9
- Formula: C32H36ClNO8
- Molecular Weight:598.08
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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FDA-Approved Drug Library
HY-L022
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Anti-Cancer Compound Library
HY-L025
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Drug Repurposing Compound Library
HY-L035
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Endocrinology Compound Library
HY-L047
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NMPA-Approved Drug Library
HY-L053
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Orally Active Compound Library
HY-L061
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FDA Approved & Pharmacopeial Drug Library
HY-L066
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Drug-Induced Liver Injury (DILI) Compound Library
HY-L076
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Tumorigenesis-Related Compound Library
HY-L100
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FDA-Approved Anticancer Drug Library
HY-L122
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Human Metabolite Library
HY-L123
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Nuclear Receptor Compound Library
HY-L126
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Off-patent Drug Library
HY-L141
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Membrane Receptor-targeted Compound Library
HY-L150
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Drug Repurposing Compound Library Plus
HY-L035P
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FDA-Approved Drug Library Plus
HY-L022P
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FDA-Approved Drug Library Mini
HY-L022M
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Mass Spectrometry Human Metabolite Library
HY-L215
Publications Citing Use of MedChemExpress (MCE) Clomiphene citrate
More
Biological Activity
Clomiphene citrate (50 μM, 1-30 min) inhibits the viability of PC3 human prostate cancer cells[1].
Clomiphene citrate (1-5 μg/mL, 18 h) induces chromosome abnormalities in mouse oocytes[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PC3 human prostate cancer cells
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Concentration:50 μM
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Incubation Time:1 min, 2 min, 5 min, 10 min, 15 min, 20 min, 30 min
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Result:Reduced cell viability by 10-50% for 2-15 min in a time-dependent manner.
Clomiphene citrate (1 mg/kg, Oral, once a day for 15 days) with carvedilol (HY-B0006) has anxiolytic potential and improved cognitive functions in letrozole(HY-14248)-induced polycystic ovary syndrome (PCOS) rats[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female ICR mice[2]
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Dosage:25 mg/kg, 50mg/kg, 100mg/kg
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Administration:Intraperitoneal injection (i.p.)
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Result:Resulted in a decrease in the number of ovulated oocytes and a significant increase in hyperploidy at 100 mg/kg.
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Animal Model:letrozole(HY-14248)-induced PCOS rats [3]
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Dosage:1 mg/kg
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Administration:Oral
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Result:Demonstrated a statistically raised NRF-2 gene expression.
Up-regulated expression of NF-κB.
Showed a reversal of the memory defect.
Down-regulated of acetylcholine esterase expression.
Chemical Information
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CAS No. 50-41-9
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Appearance Solid
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Molecular Weight 598.08
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Formula C32H36ClNO8
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Color White to off-white
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SMILES
Cl/C(C1=CC=CC=C1)=C(C2=CC=CC=C2)/C3=CC=C(OCCN(CC)CC)C=C3.OC(CC(O)=O)(C(O)=O)CC(O)=O
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Synonyms
Clomifene citrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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J Cell Mol Med
2026 Apr;30(7):e71101. PMID: 41896195 -
J Pharm Biomed Anal
Screening method of mildronate and over 300 doping agents by reversed-phase liquid chromatography-high resolution mass spectrometry. [Abstract]2021 Feb 20:195:113870. PMID: 33453569 -
Biochem Biophys Res Commun
Combinatorial screening of a panel of FDA-approved drugs identifies several candidates with anti-Ebola activities. [Abstract]2020 Feb 19;522(4):862-868. PMID: 31806372 -
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Solvent & Solubility
DMSO : ≥ 50 mg/mL (83.60 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 1 mg/mL (1.67 mM; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 7.14 mg/mL (11.94 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
[1]. Jiann B P, et al. Effect of clomiphene on Ca2+ movement in human prostate cancer cells [J]. Life Sciences, 2002, 70(26): 3167-3178. [Content Brief]
[2]. London S N, et al. Clomiphene citrate-induced perturbations during meiotic maturation and cytogenetic abnormalities in mouse oocytes in vivo and in vitro [J]. Fertility and sterility, 2000, 73(3): 620-626. [Content Brief]
[3]. Akintoye O O, et al. Synergistic action of carvedilol and clomiphene in mitigating the behavioral phenotypes of letrozole-model of PCOS rats by modulating the NRF2/NFKB pathway [J]. Life Sciences, 2023, 324: 121737. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.6720 mL | 8.3601 mL | 16.7202 mL | 41.8004 mL |
| DMSO | 5 mM | 0.3344 mL | 1.6720 mL | 3.3440 mL | 8.3601 mL |
| 10 mM | 0.1672 mL | 0.8360 mL | 1.6720 mL | 4.1800 mL | |
| 15 mM | 0.1115 mL | 0.5573 mL | 1.1147 mL | 2.7867 mL | |
| 20 mM | 0.0836 mL | 0.4180 mL | 0.8360 mL | 2.0900 mL | |
| 25 mM | 0.0669 mL | 0.3344 mL | 0.6688 mL | 1.6720 mL | |
| 30 mM | 0.0557 mL | 0.2787 mL | 0.5573 mL | 1.3933 mL | |
| 40 mM | 0.0418 mL | 0.2090 mL | 0.4180 mL | 1.0450 mL | |
| 50 mM | 0.0334 mL | 0.1672 mL | 0.3344 mL | 0.8360 mL | |
| 60 mM | 0.0279 mL | 0.1393 mL | 0.2787 mL | 0.6967 mL | |
| 80 mM | 0.0209 mL | 0.1045 mL | 0.2090 mL | 0.5225 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Clomiphene citrate Related Classifications
HY-B0463 Related Classifications
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