Clomiphene hydrochloride (Synonyms: Clomifene hydrochloride; (Z/E)-Enclomiphene hydrochloride; (Z/E)-Enclomifene hydrochloride)

Cat. No.: HY-A0287A: Data Sheet Handling Instructions
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Clomiphene (Clomifene; (Z/E)-Enclomiphene; (Z/E)-Enclomifene) hydrochloride is an orally active ovulation-inducing agent. Clomiphene hydrochloride binds to hypothalamic estrogen receptors to elevate FSH levels, and exhibits antiestrogenic or estrogenic properties. Clomiphene hydrochloride can induce erturbations during meiotic maturation and cytogenetic abnormalities in mouse oocytes. Clomiphene hydrochloride ameliorates memory impairment in PCOS models. Clomiphene hydrochloride mobilizes cytosolic calcium and reduces viability in prostate cancer cells. Clomiphene hydrochloride can be used for the research of polycystic ovary syndrome (PCOS) and prostate cancer.

For research use only. We do not sell to patients.

Clomiphene hydrochloride Chemical Structure

CAS No. : 57049-00-0

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Other In-stock Forms of Clomiphene hydrochloride:

Clomiphene citrate

Other Forms of Clomiphene hydrochloride:

5 Publications Citing Use of MCE Clomiphene hydrochloride

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Description

In Vitro

Clomiphene (1.0-5.0 μg/mL; 18 h) hydrochloride significantly increases hyperploidy and diploidy in cultured mouse germinal vesicle-stage oocytes, while reducing the proportion of metaphase I oocytes, indicating accelerated oocyte maturation^[1].
Clomiphene (10-50 μM) hydrochloride increases cytosolic free calcium levels in PC3 human prostate cancer cells with an EC₅₀ of approximately 30 μM^[4].
Clomiphene (50 μM; 2-30 min) hydrochloride induces time-dependent mild cytotoxicity in PC3 human prostate cancer cells, reducing viability by 10-50% over 2-15 minutes of incubation with no further decrease after 15 minutes^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:	PC3 human prostate cancer cells
Concentration:	50 μM
Incubation Time:	2 min; 5 min; 10 min; 15 min; 20 min; 30 min
Result:	Reduced cell viability in a time-dependent manner: 2 minutes of incubation reduced viability by 10%, 15 minutes of incubation reduced viability by 50%, and extending incubation to 30 minutes did not further reduce viability.

In Vivo

Clomiphene (25-100 mg/kg; i.p.; 3 doses over 4 hours) hydrochloride induces a significant 1.5% hyperploidy rate in mouse oocytes in vivo at the highest dose, while lower doses increase premature centromere separation and single unpaired chromatids, and all tested doses reduce ovulated oocyte counts^[1].
Clomiphene (1 mg/kg; p.o.; daily; 15 days) hydrochloride improves spatial and non-spatial memory impairment, restores reproductive hormone levels, increases serum antioxidant enzyme concentrations, and downregulates prefrontal cortex AchE gene expression in Letrozole (HY-14248)-induced PCOS rats, but does not reduce anxiety-like behavior^[2].
Clomiphene hydrochloride decreases serum gonadotropin levels and reduces testosterone secretion in rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	ICR (female, 8-12 weeks, 25-34 g, ovarian stimulation with PMSG and hCG)^[1]
Dosage:	25, 50, 100 mg/kg
Administration:	i.p.; 3 doses over 4 hours
Result:	Increased hyperploidy to 1.5% (4 of 265 analyzed oocytes) and reduced average number of ovulated oocytes per mouse to 20.5 at 100 mg/kg. Increased premature centromere separation to 5.0% (15 of 300 analyzed oocytes) and reduced average ovulated oocytes per mouse to 35.0 at 25 mg/kg. Increased premature centromere separation to 3.6% (12 of 329 analyzed oocytes), increased single unpaired chromatids to 1.8% (6 of 329 analyzed oocytes), and reduced average ovulated oocytes per mouse to 37.7 at 50 mg/kg.

Animal Model:	Wistar (female, 190-210 g, Letrozole-induced PCOS)^[2]
Dosage:	1 mg/kg
Administration:	p.o.; daily; 15 days
Result:	Significantly increased spontaneous alternation performance count and percentage in the Y maze compared to PCOS-only rats. Increased serum superoxide dismutase (SOD) and catalase concentrations compared to PCOS-only rats. Showed weak non-significant reduction in serum interleukin-1β (IL-1B) levels compared to PCOS-only rats. Reversed upregulated acetylcholine esterase (AchE) gene expression in the prefrontal cortex compared to PCOS-only rats. Reduced follicle-stimulating hormone (FSH) to 0.31 mIU/mL, reduced luteinizing hormone (LH) to 3.14 mIU/mL, increased estradiol to 27.68 ng/mL, and reduced testosterone to 0.28 ng/mL compared to PCOS-only rats. Did not reduce anxiety-like behavior, as anxiety index values remained statistically high compared to control rats.

Molecular Weight

442.42

Formula

C₂₆H₂₉Cl₂NO

CAS No.

57049-00-0

SMILES

Cl/C(C1=CC=CC=C1)=C(C2=CC=C(OCCN(CC)CC)C=C2)/C3=CC=CC=C3.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. London SN, et al. Clomiphene citrate-induced perturbations during meiotic maturation and cytogenetic abnormalities in mouse oocytes in vivo and in vitro. Fertil Steril. 2000;73(3):620-626. [Content Brief]

[2]. Akintoye OO, et al. Synergistic action of carvedilol and clomiphene in mitigating the behavioral phenotypes of letrozole-model of PCOS rats by modulating the NRF2/NFKB pathway. Life Sci. 2023;324:121737. [Content Brief]

[3]. Legro R S, et al. Clomiphene, metformin, or both for infertility in the polycystic ovary syndrome[J]. New England Journal of Medicine, 2007, 356(6): 551-566.

[4]. Jiann BP, et al. Effect of clomiphene on Ca(2+) movement in human prostate cancer cells. Life Sci. 2002;70(26):3167-3178. [Content Brief]

Clomiphene hydrochloride Related Classifications

Metabolic Disease Cancer

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Clomiphene57049-00-0Clomifene(Z/E)-Enclomiphene(Z/E)-EnclomifeneEstrogen Receptor/ERRoralinfertilityovulationsafewell-toleratedefficaciousInhibitorinhibitorinhibit

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