Cloprostenol isopropyl ester
Based on 1 Customer Validation
Cloprostenol isopropyl ester, a prostaglandin F2α analogs, is the intermediate of (+)-Cloprostenol (HY-107381). Cloprostenol isopropyl ester is a FP receptor agonist with a Ki value of 28 nM.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 157283-66-4
- Formula: C25H35ClO6
- Molecular Weight:466.99
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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FP |
Cloprostenol isopropyl ester (1 μg, 30 μL; injected into the eye; for 2, 4 and 6 h) causes pupil constriction in the cat. Cloprostenol isopropyl ester has an efficacious ocular hypotensive in the lasered monkey[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male mice (50–80 days)[1]
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Dosage:25 μg/kg
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Administration:Intraperitoneal injection; daily, for 4 weeks
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Result:Stimulated the androgen synthesis in a time-dependent manner.
Chemical Information
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CAS No. 157283-66-4
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Appearance Oil
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Molecular Weight 466.99
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Formula C25H35ClO6
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Color Colorless to light yellow
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SMILES
ClC1=CC=CC(OC[C@H](O)/C=C/[C@@H]2[C@H]([C@H](C[C@H]2O)O)C/C=C\CCCC(OC(C)C)=O)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (214.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Sava A, et, al. Electron microscopic aspects of the effects of certain prostaglandin analogs on mouse testes. Rom J Morphol Embryol. 2015;56(2 Suppl):771-5. [Content Brief]
[2]. Hellberg MR, et, al. Identification and characterization of the ocular hypotensive efficacy of travoprost, a potent and selective FP prostaglandin receptor agonist, and AL-6598, a DP prostaglandin receptor agonist. Surv Ophthalmol. 2002 Aug;47 Suppl 1:S13-33. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.1414 mL | 10.7069 mL | 21.4137 mL | 53.5343 mL |
| 5 mM | 0.4283 mL | 2.1414 mL | 4.2827 mL | 10.7069 mL | |
| 10 mM | 0.2141 mL | 1.0707 mL | 2.1414 mL | 5.3534 mL | |
| 15 mM | 0.1428 mL | 0.7138 mL | 1.4276 mL | 3.5690 mL | |
| 20 mM | 0.1071 mL | 0.5353 mL | 1.0707 mL | 2.6767 mL | |
| 25 mM | 0.0857 mL | 0.4283 mL | 0.8565 mL | 2.1414 mL | |
| 30 mM | 0.0714 mL | 0.3569 mL | 0.7138 mL | 1.7845 mL | |
| 40 mM | 0.0535 mL | 0.2677 mL | 0.5353 mL | 1.3384 mL | |
| 50 mM | 0.0428 mL | 0.2141 mL | 0.4283 mL | 1.0707 mL | |
| 60 mM | 0.0357 mL | 0.1784 mL | 0.3569 mL | 0.8922 mL | |
| 80 mM | 0.0268 mL | 0.1338 mL | 0.2677 mL | 0.6692 mL | |
| 100 mM | 0.0214 mL | 0.1071 mL | 0.2141 mL | 0.5353 mL |
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