Coronalolide
Coronalolide is a natural triterpene with anticancer effects. Coronalolide exhibits a broad cytotoxic activity for human breast (BT474), gastric (KATO-3), lung (CHAGO), colon (SW-620), and liver (Hep-G2) cancer cell lines, with IC50 values of 6.59 µg/mL, 5.85 µg/mL, 5.42 µg/mL, 4.98 µg/mL, and 6.41 µg/mL, respectively.
For research use only. We do not sell to patients.
- CAS No.: 268214-51-3
- Formula: C30H42O5
- Molecular Weight:482.65
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BT-474 | IC50 |
6.59 μg/mL
Compound: 7
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Cytotoxicity against human BT474 cells after 3 days by MTT assay
Cytotoxicity against human BT474 cells after 3 days by MTT assay
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[PMID: 19456120] |
| HepG2 | IC50 |
6.41 μg/mL
Compound: 7
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Cytotoxicity against human HepG2 cells after 3 days by MTT assay
Cytotoxicity against human HepG2 cells after 3 days by MTT assay
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[PMID: 19456120] |
| SW-620 | IC50 |
4.98 μg/mL
Compound: 7
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Cytotoxicity against human SW620 cells after 3 days by MTT assay
Cytotoxicity against human SW620 cells after 3 days by MTT assay
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[PMID: 19456120] |
Chemical Information
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CAS No. 268214-51-3
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Molecular Weight 482.65
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Formula C30H42O5
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SMILES
OC(CC[C@@]12[C@]3([C@](C[C@](O4)([H])[C@@]2([H])C(C4=O)=C)([H])[C@]5([C@@](C)([C@]([C@H](C)CC/C=C(C)/C=O)([H])CC5)CC3)C)C1)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Coronalolide Related Classifications
HY-N3626 Related Classifications
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