Art. -Nr.: HY-N0326

L-Methionine

10 Cited Publications

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Based on 10 publication(s) in Google Scholar

L-Methionine is an L-isomer of orally active Methionine, an essential amino acid. Methionine is a strong liver antidote that acts as a liver protector. L-Methionine can inhibit cell proliferation and induce cell apoptosis. L-Methionine has antitumor and antioxidant activity.

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Reinheit: 99.84%
CAS. Nr.: 63-68-3
Formel: C₅H₁₁NO₂S
Molecular Weight:149.22

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

L-Methionine Chemical Structure

CAS. Nr.: 63-68-3

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Use of MCE Products

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (10)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

In Vitro

L-Methionine (5 mg/mL, 7 or 3 days) can inhibit the proliferation of BXPC-3 (mutant p53) and HPAC (wild-type p53) pancreatic cancer cells, interfere with cell cycle, and induce apoptosis of BXPC-3 cells^[1].
L-Methionine (0.3-10 mM, 24 h) reduces the formation of free radicals in endothelial cells by inducing heme oxidase-1 and ferritin^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

BXPC-3, HPAC
Concentration:

5 mg/mL
Incubation Time:

7 or 3 days
Result:

Reduced growth of BXPC-3 cells by 31 and 32%, respectively.
Reduced growth of HPAC cells by 35% and 18%, respectively.
Reduced S to G2 transition in BXPC-3 cells and intervened in both G1–S and S–G2 transitions in HPAC cells.
Increased late cell apoptosis at a percentage of 76% in BXPC-3 cells.

Western Blot Analysis^[2]

Cell Line:

ECV304
Concentration:

0.3, 1, 3, 5, 10 mM
Incubation Time:

24 h
Result:

Increased the level of HO-1 protein up to 9.5-fold and the HO-1 activity.

In Vivo

L-Methionine (215, 268.8, 322.5, 430 mg/kg, orally, for 14 consecutive days) induces endogenous antioxidant activity by activating the Nrf2-ARE pathway and inhibits ROS-induced oxidative stress in growing rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Wistar rats (body weight 70-80 g)^[3]
Dosage:

215,268.8, 322.5, 430 mg/kg
Administration:

p.o.
Result:

Reduced the hepatic ROS levels and the hepatic MDA contents.
Increased the hepatic T-AOC, CAT and T-SOD.
Increased the hepatic activity of GCL, GR, GST and GPx.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 63-68-3
Appearance Solid
Molecular Weight 149.22
Formel C₅H₁₁NO₂S
Color White to off-white
SMILES

N[C@@H](CCSC)C(O)=O
Structure Classification
- Amino acids
Initial Source
- Microorganisms
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (10)

All WBELISAIHCIn Vivo Efficacy StudyFlow Cytometry

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Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Nat Commun

Methylcobalamin protects against liver failure via engaging gasdermin E. [Abstract]

2025 Jan 31;16(1):1233. PMID: 39890804
Adv Sci (Weinh)

Cars2-Mediated Cysteine Catabolism Drives Brown Fat Development and Thermogenesis Through Persulfidating EBF2. [Abstract]

2026 Mar 18:e22690. PMID: 41849685
Adv Sci (Weinh)

Methionine Metabolism Dictates PCSK9 Expression and Antitumor Potency of PD-1 Blockade in MSS Colorectal Cancer. [Abstract]

2025 Mar 24:e2501623. PMID: 40125618

L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar 24:e2501623. [Abstract]

The expression and secretion of PCSK9 in human with methionine deprivation for 6 h and then methionine (0-50 μM) supplementation for 24h. Immunoblotting analysis of PCSK9 in human CRC cell lines (SW480, SW620, HCT116, and LoVo).

L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar 24:e2501623. [Abstract]

L-Methionine (0, 20, 50 μM). ELISA analysis of PCSK9 in culture supernatant of human CRC cell lines (SW480, SW620, HCT116, and LoVo).

L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar 24:e2501623. [Abstract]

Mice were housed on either a normal diet (ND) with 0.86% methionine or a dietary methionine restriction (DMR) diet with 0.12% methionine from the day after injection until the endpoints. Immunohistochemistry analysis of PCSK9 in tumors from MC38‐ (n = 9) and Colon 26‐ (n = 4 (ND), n = 8 (DMR)) bearing mice fed with normal diet and methionine restriction diet (left). The abundance of PCSK9 was assessed (right).

L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar 24:e2501623. [Abstract]

Mice were housed on either a normal diet (ND) with 0.86% methionine or a dietary methionine restriction (DMR) diet with 0.12% methionine from the day after injection until the endpoints. Tumor volume of PCSK9‐KD (n = 10) or PCSK9‐KO (n = 9) MC38‐bearing mice fed with normal diet and methionine restriction diet. EAA, essential amino acid; NEEA, non‐essential amino acid; CM, complete medium; ND, normal diet; DMR, dietary methionine restriction.

L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar 24:e2501623. [Abstract]

Mice were housed on either a normal diet (ND) with 0.86% methionine or a dietary methionine restriction (DMR) diet with 0.12% methionine from the day after injection until the endpoints. Flow cytometric analysis of TNFα and Granzyme B in intratumoral CD8+T cells from Colon 26‐bearing mice fed with a methionine restriction diet alone or in combination with anti‐PD‐1 therapy.
Neoplasia

L-methionine promotes CD8+ T cells killing hepatocellular carcinoma by inhibiting NR1I2/PCSK9 signaling. [Abstract]

2025 Mar 29:64:101160. PMID: 40158232
Emerg Microbes Infect

SARS-CoV-2 and HCoV-OC43 regulate host m6A modification via activation of the mTORC1 signaling pathway to facilitate viral replication. [Abstract]

2025 Jan 2:2447620. PMID: 39745173
Molecules

Seeking Correlation Among Porin Permeabilities and Minimum Inhibitory Concentrations Through Machine Learning: A Promising Route to the Essential Molecular Descriptors. [Abstract]

2025 Mar 9;30(6):1224. PMID: 40142001
J Chromatogr A

Quantification of five intracellular and extracellular methionine pathway intermediates using stable isotope dilution UHPLC-MS/MS. [Abstract]

2025 May 10:1755:466036. PMID: 40398135
Sci Rep

Interaction between ELMO1 DNA methylation and Med31 promotes H. pylori-induced gastric cancer EMT and intestinal metaplasia via M2 polarization. [Abstract]

2026 Jan 14;16(1):5201. PMID: 41535333
bioRxiv

2024 August 18.

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : 10 mg/mL (67.02 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.7015 mL

33.5076 mL

67.0151 mL

5 mM

1.3403 mL

6.7015 mL

13.4030 mL

10 mM

0.6702 mL

3.3508 mL

6.7015 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 12.5 mg/mL (83.77 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Benavides MA, et al. L-Methionine inhibits growth of human pancreatic cancer cells. Anticancer Drugs. 2014 Feb;25(2):200-3. [Content Brief]

[2]. Erdmann K, et al. L-methionine reduces oxidant stress in endothelial cells: role of heme oxygenase-1, ferritin, and nitric oxide. AAPS J. 2005 Aug 29;7(1):E195-200. [Content Brief]

[3]. Wang Z, et al. l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats. J Sci Food Agric. 2019 Aug 15;99(10):4849-4862. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	6.7015 mL	33.5076 mL	67.0151 mL	167.5379 mL
	5 mM	1.3403 mL	6.7015 mL	13.4030 mL	33.5076 mL
	10 mM	0.6702 mL	3.3508 mL	6.7015 mL	16.7538 mL
	15 mM	0.4468 mL	2.2338 mL	4.4677 mL	11.1692 mL
	20 mM	0.3351 mL	1.6754 mL	3.3508 mL	8.3769 mL
	25 mM	0.2681 mL	1.3403 mL	2.6806 mL	6.7015 mL
	30 mM	0.2234 mL	1.1169 mL	2.2338 mL	5.5846 mL
	40 mM	0.1675 mL	0.8377 mL	1.6754 mL	4.1884 mL
	50 mM	0.1340 mL	0.6702 mL	1.3403 mL	3.3508 mL
	60 mM	0.1117 mL	0.5585 mL	1.1169 mL	2.7923 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

L-Methionine

10 Cited Publications

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L-Methionine

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Publications Citing Use of MedChemExpress (MCE) L-Methionine

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (10)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

L-Methionine Related Classifications

HY-N0326 Related Classifications