diABZI-V/C-Mal
Based on 1 Customer Validation
diABZI-V/C-Mal is a STING agonist (with a STING EC50 of 314 nM in TH1 dual reporter cells) and a Cathepsin B substrate. diABZI-V/C-Mal activates STING, thereby triggering the IRF3 signaling pathway. diABZI-V/C-Mal is cleaved by Cathepsin B to regenerate diABZI-NH2.
For research use only. We do not sell to patients.
- Purity : 99.28%
- CAS No.: 3035557-55-9
- Formula: C67H83N19O14
- Molecular Weight:1378.49
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
IC50 & Target
[1]|
Cathepsin B |
In Vitro
diABZI-V/C-Mal (50 μM; 48 h) undergoes cleavage by activated cathepsin B in a cell-free system, as evidenced by a shift in molecular weight matching the liberated diABZI-NH2 fragment[1].
diABZI-V/C-Ma activates STING-mediated IRF3 signaling in THP-1 dual reporter cells with potency comparable to diABZI-NH2[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 3035557-55-9
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Appearance Solid
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Molecular Weight 1378.49
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Formula C67H83N19O14
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Color White to off-white
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SMILES
O=C(C1=CC(C)=NN1CC)NC2=NC3=CC(C(N)=O)=CC(OC)=C3N2C/C=C/CN4C5=C(OCCCNC(OCC6=CC=C(C=C6)NC([C@H](CCCNC(N)=O)NC([C@H](C(C)C)NC(CCCCCN7C(C=CC7=O)=O)=O)=O)=O)=O)C=C(C(N)=O)C=C5N=C4NC(C8=CC(C)=NN8CC)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (72.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Naïve CD4+ T-cell subset differentiation/polarization
Naïve CD4+ T-cell subset differentiation/polarization is an in vitro assay in which purified naïve CD4+ T cells are activated through TCR and CD28 costimulation and cultured with defined cytokines and neutralizing antibodies to generate Th0, Th1, Th2, Th17, or induced Treg-like populations. Differentiation is detected by subset-associated cytokines and transcription factors: IFN-γ/T-bet for Th1, IL-4/GATA3 for Th2, IL-17A/RORγt for Th17, and Foxp3 for induced Treg cells. The assay readout is usually generated by intracellular cytokine staining after restimulation, transcription-factor staining by flow cytometry, ELISA of secreted cytokines, or gene-expression analysis. The result reflects cytokine-directed lineage commitment or polarization rather than antigen-specific immune protection by itself.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7254 mL | 3.6272 mL | 7.2543 mL | 18.1358 mL |
| 5 mM | 0.1451 mL | 0.7254 mL | 1.4509 mL | 3.6272 mL | |
| 10 mM | 0.0725 mL | 0.3627 mL | 0.7254 mL | 1.8136 mL | |
| 15 mM | 0.0484 mL | 0.2418 mL | 0.4836 mL | 1.2091 mL | |
| 20 mM | 0.0363 mL | 0.1814 mL | 0.3627 mL | 0.9068 mL | |
| 25 mM | 0.0290 mL | 0.1451 mL | 0.2902 mL | 0.7254 mL | |
| 30 mM | 0.0242 mL | 0.1209 mL | 0.2418 mL | 0.6045 mL | |
| 40 mM | 0.0181 mL | 0.0907 mL | 0.1814 mL | 0.4534 mL | |
| 50 mM | 0.0145 mL | 0.0725 mL | 0.1451 mL | 0.3627 mL | |
| 60 mM | 0.0121 mL | 0.0605 mL | 0.1209 mL | 0.3023 mL |
diABZI-V/C-Mal Related Classifications
HY-164278 Related Classifications
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