2. Metabolic Enzyme/Protease Neuronal Signaling Membrane Transporter/Ion Channel Immunology/Inflammation
  3. Angiotensin-converting Enzyme (ACE) Calcium Channel NO Synthase
  4. Dipeptide 2

Dipeptide 2  (Synonyms: N-Valyltryptophan; Val-Trp)

Cat. No.: HY-118060 Purity: 98.12%
COA
SDS
Handling Instructions Technical Support

Dipeptide 2 (N-Valyltryptophan) is an orally active, competitive angiotensin-converting enzyme (ACE) inhibitory peptide with an IC50 of 10.50 μM. Dipeptide 2 reduces intracellular Calcium ions. Dipeptide 2 significantly increases the content of NO, inhibits the production of ET-1, and induces the phosphorylation of eNOS. Dipeptide 2 decreases the systolic and diastolic blood pressure of spontaneously hypertensive rats.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Dipeptide 2

Dipeptide 2 Chemical Structure

CAS No. : 24587-37-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 g In-stock
5 g In-stock
25 g In-stock
50 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Dipeptide 2:

Dipeptide 2 Related Antibodies

Top Publications Citing Use of Products

View All Calcium Channel Isoform Specific Products:

View All Isoforms
N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

View All NO Synthase Isoform Specific Products:

View All Isoforms
nNOS iNOS eNOS

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Dipeptide 2 (N-Valyltryptophan) is an orally active, competitive angiotensin-converting enzyme (ACE) inhibitory peptide with an IC50 of 10.50 μM. Dipeptide 2 reduces intracellular Calcium ions. Dipeptide 2 significantly increases the content of NO, inhibits the production of ET-1, and induces the phosphorylation of eNOS. Dipeptide 2 decreases the systolic and diastolic blood pressure of spontaneously hypertensive rats[1].

IC50 & Target[1]

eNOS

 

Cellular Effect
Cell Line Type Value Description References
MDCK EC50
0.04 mM
Compound: 79
Activation of human PEPT1 expressed in MDCK cells
Activation of human PEPT1 expressed in MDCK cells
[PMID: 16759105]
MDCK IC50
0.1 mM
Compound: 79
Binding affinity to human PEPT1 assessed as inhibition of [14C]Gly-Sar uptake in MDCK cells
Binding affinity to human PEPT1 assessed as inhibition of [14C]Gly-Sar uptake in MDCK cells
[PMID: 16759105]
In Vitro

Dipeptide 2 potently inhibits purified ACE with an IC50 of 10.50 ± 0.97 μM[2].
Dipeptide 2 (12.5-50 μM; 24 h) is non-toxic to HUVECs[2].
Dipeptide 2 (12.5-50 μM; 4 h) reduces intracellular calcium concentration in Ang I-induced HUVECs, promoting vascular smooth muscle relaxation[2].
Dipeptide 2 (12.5-50 μM; 4 h) promotes NO production in Ang I-induced HUVECs, with a 12.9% increase at 50 μM[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dipeptide 2 Related Antibodies

Western Blot Analysis[2]

Cell Line: Ang I-induced human umbilical vein endothelial cells (HUVECs)
Concentration: 12.5-50 μM
Incubation Time: 4 h
Result: Significantly inhibited ET-1 protein expression and stimulated eNOS phosphorylation compared to the Ang I-only group.
Caused a 60.4% reduction in ET-1 and a 92.9% increase in p-eNOS protein expression in the 50 μM group.
In Vivo

Dipeptide 2 (10-40 mg/kg; p.o.; once daily; for 12 consecutive days) exerts significant in vivo hypotensive activity in spontaneously hypertensive rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: SHRs (male, 8 weeks old, 220 ± 20 g, spontaneously hypertensive model)[2]
Dosage: 10 mg/kg; 40 mg/kg
Administration: p.o.; once daily; 12 days
Result: Significantly suppressed diastolic blood pressure (DBP) in SHRs between days 8 and 12, with DBP reductions ranging from 3 to 27 mmHg.
Produced a maximum DBP reduction of 27 mmHg on day 12 at 40 mg/kg.
Reduced systolic blood pressure (SBP) by 32 mmHg on day 12.
Molecular Weight

303.36

Formula

C16H21N3O3
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Val-Trp
Sequence Shortening

VW
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (41.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2964 mL 16.4821 mL 32.9641 mL
5 mM 0.6593 mL 3.2964 mL 6.5928 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.12%

SDS (393 KB)

COA (255 KB) HNMR (134 KB) LCMS (331 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2964 mL 16.4821 mL 32.9641 mL 82.4103 mL
5 mM 0.6593 mL 3.2964 mL 6.5928 mL 16.4821 mL
10 mM 0.3296 mL 1.6482 mL 3.2964 mL 8.2410 mL
15 mM 0.2198 mL 1.0988 mL 2.1976 mL 5.4940 mL
20 mM 0.1648 mL 0.8241 mL 1.6482 mL 4.1205 mL
25 mM 0.1319 mL 0.6593 mL 1.3186 mL 3.2964 mL
30 mM 0.1099 mL 0.5494 mL 1.0988 mL 2.7470 mL
40 mM 0.0824 mL 0.4121 mL 0.8241 mL 2.0603 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Dipeptide 2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dipeptide 224587-37-9N-Valyltryptophan Val-TrpDipeptide2Dipeptide-2Angiotensin-converting Enzyme (ACE)Calcium ChannelNO SynthaseCa2+ channelsCa channelsNitric oxide synthasesNOSangiotensin Ispontaneously hypertensive ratshuman endothelial cellshypertensionangiotensin-converting enzymebradykininACEHUVECsangiotensin IIeNOSInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Dipeptide 2
Cat. No.:
HY-118060
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.