5-Bromouridine
Based on 2 publication(s) in Google Scholar
5-Bromouridine is a brominated uridine nucleoside. The triphosphate form of 5-Bromouridine, 5BrdUTP, can substitute for TTP as a substrate for DNA polymerase and be incorporated into nascent DNA strands, rendering the resulting 5-bromouridine-labeled DNA photosensitive to 300 nm UV irradiation; upon UV irradiation, the 5BrdU signal decreases and dU is generated, accompanied by DNA strand breaks and cross-link formation. 5-Bromouridine is useful for studies on nucleoside structure and halogenated nucleoside-labeled nucleic acids.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 957-75-5
- Formula: C9H11BrN2O6
- Molecular Weight:323.10
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-W014306
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5-Fluorouracil -
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Fialuridine -
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3′-Deoxythymidine -
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Capecitabine -
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5-Fluorouracil (Standard) -
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2′-O-Methyluridine
Publications Citing Use of MedChemExpress (MCE) 5-Bromouridine
MoreAll DNA/RNA Synthesis Isoforms
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Biological Activity
Description
In Vitro
300 nm irradiation of 5-bromouridine-labeled 500 bp DNA for 60 min induces single-strand breaks, cross-links, and heat-labile sites, with greater damage observed at 25 °C than at 0 °C[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 957-75-5
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Appearance Solid
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Molecular Weight 323.10
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Formula C9H11BrN2O6
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Color White to off-white
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C(NC(C(Br)=C2)=O)=O)O[C@@H]1CO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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ACS Chem Biol
Li-BrU-seq: A Low-Input and Simplified Metabolic Labeling Method for Nascent RNA Sequencing. [Abstract]2026 Mar 20;21(3):601-612. PMID: 41773915 -
Virology
5-Bromo-2'-deoxyuridine inhibits African swine fever virus (ASFV) replication via interfering viral DNA replication and suppressing the formation of viral factories. [Abstract]2024 Dec:600:110237. PMID: 39288610
Solvent & Solubility
In Vitro:
H2O : 200 mg/mL (619.00 mM; Need ultrasonic)
DMSO : 100 mg/mL (309.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.74 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.74 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.0950 mL | 15.4751 mL | 30.9502 mL | 77.3754 mL |
| 5 mM | 0.6190 mL | 3.0950 mL | 6.1900 mL | 15.4751 mL | |
| 10 mM | 0.3095 mL | 1.5475 mL | 3.0950 mL | 7.7375 mL | |
| 15 mM | 0.2063 mL | 1.0317 mL | 2.0633 mL | 5.1584 mL | |
| 20 mM | 0.1548 mL | 0.7738 mL | 1.5475 mL | 3.8688 mL | |
| 25 mM | 0.1238 mL | 0.6190 mL | 1.2380 mL | 3.0950 mL | |
| 30 mM | 0.1032 mL | 0.5158 mL | 1.0317 mL | 2.5792 mL | |
| 40 mM | 0.0774 mL | 0.3869 mL | 0.7738 mL | 1.9344 mL | |
| 50 mM | 0.0619 mL | 0.3095 mL | 0.6190 mL | 1.5475 mL | |
| 60 mM | 0.0516 mL | 0.2579 mL | 0.5158 mL | 1.2896 mL | |
| 80 mM | 0.0387 mL | 0.1934 mL | 0.3869 mL | 0.9672 mL | |
| 100 mM | 0.0310 mL | 0.1548 mL | 0.3095 mL | 0.7738 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
5-Bromouridine Related Classifications
HY-W009163 Related Classifications
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Keywords
- 5-Bromouridine
- 957-75-5
- Nucleoside Antimetabolite/Analog
- DNA/RNA Synthesis
- inter-strand cross-links
- synthetic nucleoside derivative
- DNA single- and double-strand breaks
- malignant brain tumors
- DNA radiosensitizer
- C2'-endo ribose sugar pucker
- monoclinic unit cell
- brominated pyrimidine nucleoside analog
- thermostable DNA polymerases
- chromosomal aberrations
- Inhibitor
- inhibitor
- inhibit