Cat. No.: HY-181056

EGFR/COX-2-IN-3

Customer Review

Based on 1 Customer Validation

EGFR/COX-2-IN-3 is a selective EGFR/COX-2 dual inhibitor with IC₅₀ values of 0.098 μM and 0.05 μM, respectively. EGFR/COX-2-IN-3 is predicted to have oral activity and blood-brain barrier permeability. EGFR/COX-2-IN-3 exhibits both potent analgesic activity and anti-glioblastoma effects. EGFR/COX-2-IN-3 can be used in the research of tumors such as glioblastoma.

For research use only. We do not sell to patients.

Purity: 99.76%
CAS No.: 3121638-16-9
Formula: C₂₂H₂₅N₅O
Molecular Weight:375.47

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

EGFR/COX-2-IN-3 Chemical Structure

CAS No.: 3121638-16-9

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Antibody-Drug Conjugates

One-stop solution for XDC (ADC/PDC/RDC/ApDC/FDC/ISAC/VDC/SMDC)

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

COX-2

0.15 μM (IC₅₀)

All EGFR Isoforms :

All COX Isoforms :

In Vitro

EGFR/COX-2-IN-3 (Compound 10a) exhibits potent cytotoxicity against U-251 and SNB-75, with IC₅₀ values of 8.8 μM and 2.6 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

EGFR/COX-2-IN-3 exhibits analgesic activity in the mouse hot-plate test, with an AUC of 37.83, which is superior to that of Celecoxib (HY-14398)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 3121638-16-9
Appearance Solid
Molecular Weight 375.47
Formula C₂₂H₂₅N₅O
Color White to off-white
SMILES

O=C(CN1CCN(C)CC1)NC2=CC(C3=CC=CC=C3)=NN2C4=CC=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (66.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6633 mL

13.3166 mL

26.6333 mL

5 mM

0.5327 mL

2.6633 mL

5.3267 mL

10 mM

0.2663 mL

1.3317 mL

2.6633 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Farag KO, et al. Pyrazole derivatives as dual COX-2/EGFR inhibitors with anticancer potential: Optimization of difenamizole Analogs. Bioorg Chem. 2026;170:109444. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6633 mL	13.3166 mL	26.6333 mL	66.5832 mL
	5 mM	0.5327 mL	2.6633 mL	5.3267 mL	13.3166 mL
	10 mM	0.2663 mL	1.3317 mL	2.6633 mL	6.6583 mL
	15 mM	0.1776 mL	0.8878 mL	1.7756 mL	4.4389 mL
	20 mM	0.1332 mL	0.6658 mL	1.3317 mL	3.3292 mL
	25 mM	0.1065 mL	0.5327 mL	1.0653 mL	2.6633 mL
	30 mM	0.0888 mL	0.4439 mL	0.8878 mL	2.2194 mL
	40 mM	0.0666 mL	0.3329 mL	0.6658 mL	1.6646 mL
	50 mM	0.0533 mL	0.2663 mL	0.5327 mL	1.3317 mL
	60 mM	0.0444 mL	0.2219 mL	0.4439 mL	1.1097 mL

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

EGFR/COX-2-IN-3

Bioactive Compound Library Max

Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Protein Tyrosine Kinase Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Pyroptosis Compound Library

Anti-Hepatitis C Virus Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Antidepressant Compound Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Cytokine Inhibitors Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Radioprotector Library

MCE Bioactive Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

All EGFR Isoforms

All COX Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

EGFR/COX-2-IN-3 Related Classifications

HY-181056 Related Classifications