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Art. -Nr.: HY-P990019

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Biologically Validated

Epacmarstobart

Name: Epacmarstobart
Brand: MedChemExpress
SKU: HY-P990019
Price: 300.00 USD
Availability: PreOrder

(Synonyms: FSI-189; GS-0189)

Epacmarstobart (FSI-189; GS-0189) is an anti-SIRPα humanized antibody that blocks the CD47-SIRPα interaction. Epacmarstobart binds to human SIRPαV1 and SIRPαV2 with K_d values of 4.3 nM and 332 nM, respectively. Epacmarstobart enhances tumor cell phagocytosis in vitro in a SIRPα variant-dependent manner. Epacmarstobart can be used for research on non-Hodgkin lymphoma.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr.: 2518200-89-8
Molecular Weight:144.94 kDa

Speicherung:

Shipping with dry ice

Please store the product under the recommended conditions in the Certificate of Analysis.

Epacmarstobart Chemical Structure

CAS. Nr.: 2518200-89-8

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Verfügbarkeit

Menge

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Clinical Trial
- Gene ID
- Accession
- Conjugated
- Reconstitution
- Format
- Anwendung
- Verified Bioactivity
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Isotype

Human IgG1 kappa

Recommend Isotype Controls

Human IgG1 kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

SIRPa/CD172a

Cellular Effect

Cell Line	Type	Value	Description	References
Monocyte	EC50	0.024 μg/mL	Receptor occupancy on human CD14+ monocytes from healthy donors with SIRPαV1/V1 genotype assessed by free-receptor format using allophycocyanin-conjugated anti-human CD172ab antibody and mean fluorescence intensity. Receptor occupancy on human CD14+ monocytes from healthy donors with SIRPαV1/V1 genotype assessed by free-receptor format using allophycocyanin-conjugated anti-human CD172ab antibody and mean fluorescence intensity.	37206279
Monocyte	EC50	0.071 μg/mL	Receptor occupancy on human CD14+ monocytes from healthy donors with SIRPαV1/V2 genotype assessed by free-receptor format using allophycocyanin-conjugated anti-human CD172ab antibody and mean fluorescence intensity. Receptor occupancy on human CD14+ monocytes from healthy donors with SIRPαV1/V2 genotype assessed by free-receptor format using allophycocyanin-conjugated anti-human CD172ab antibody and mean fluorescence intensity.	37206279
Monocyte	EC50	2.44 μg/mL	Receptor occupancy on human CD14+ monocytes from healthy donors with SIRPαV2/V2 genotype assessed by free-receptor format using allophycocyanin-conjugated anti-human CD172ab antibody and mean fluorescence intensity. Receptor occupancy on human CD14+ monocytes from healthy donors with SIRPαV2/V2 genotype assessed by free-receptor format using allophycocyanin-conjugated anti-human CD172ab antibody and mean fluorescence intensity.	37206279

In Vitro

Innate ImmunityCytokines & ChemokinesAnti-cancer

Innate Immunity

Epacmarstobart (GS-0189) (0.3-3 μM) binds recombinant SIRPαV1 with a K_D of 4.3 nM and SIRPαV2 with a K_D of 332 nM, showing 77-fold higher affinity for SIRPαV1^[1].
Epacmarstobart occupies CD14⁺ monocytes from healthy donors in a SIRPα variant-dependent manner, with receptor occupancy (RO) EC₅₀ values of 0.024 μg/mL for SIRPαV1/V1, 0.071 μg/mL for SIRPαV1/V2, and 2.44 μg/mL for SIRPαV2/V2^[1].

Innate Immunity

Cytokines & Chemokines

Anti-cancer

Epacmarstobart potentiates DLD-1 phagocytosis by PBMC-derived macrophages in a SIRPα variant-dependent manner, with EC₅₀ values of 0.02 μg/mL for SIRPαV1/V1 versus 13.8 and 9.5 μg/mL for SIRPαV1/V2 and SIRPαV2/V2^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Phagocytosis Functional Assay

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991			2011-05	PHASE1

Gene ID

140885 [NCBI]

Accession

P78324

Conjugated

Unconjugated

Reconstitution

The product can be reconstituted/diluted with sterile PBS or saline.

Format

Human IgG1 kappa

Anwendung

ELISA, FACS, Functional assay

Verified Bioactivity

Flow Cytometry analysis of THP-1 cells labelling SIRPa (red) with Epacmarstobart (anti-SIRPa) (HY-P990019). Cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99991, blue) was used as the isotype control.

Chemical Information

CAS. Nr. 2518200-89-8
Appearance Liquid
Molecular Weight 144.94 kDa
Color Colorless to light yellow
SMILES

[Epacmarstobart]
Synonyms

FSI-189; GS-0189
Versand

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Inhibitory Antibodies User Guide (603 KB)

Verweise

[1]. Narkhede M, et al. A phase 1 first-in-human study of GS-0189, an anti-signal regulatory protein alpha (SIRPα) monoclonal antibody, in patients with relapsed/refractory (R/R) non-Hodgkin lymphoma (NHL). EJHaem. 2023 May;4(2):370-380. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

MOQ

Minimum order quantity

100 mg

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Amount: USD 0.00

Biologische Aktivität
- Beschreibung
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vitro
- Clinical Trial
- Gene ID
- Accession
- Conjugated
- Reconstitution
- Format
- Anwendung
- Biological Activity
Chemical Information
Reinheit & Dokumentation
Verweise

1/16

Human IgG1 kappa
1/16

Flow Cytometry analysis of THP-1 cells labelling SIRPa (red) with Epacmarstobart (anti-SIRPa) (HY-P990019). Cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99991, blue) was used as the isotype control.
1/16

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Epacmarstobart